Enrichment of Marrow Hemopoietic Progenitor Cells using a Blood Cell Processor

A total of 93 bone marrows (BM) from normal donors and patients were processed using the IBM-COBE 2991 blood cell washer to produce a concentrated buffy coat (BC) for either bone marrow transplantation (BMT) or cryopreservation for subsequent autologous BMT. The reduction in volume was 73.3 ± 8.5% and nucleated blood cells (NBC) recovery was 87.1 ± 9.1% of original marrow. Red blood cell (RBC) and platelet (PLT) contamination was reduced 64.5 ± 10.9% and 41.2 ± 24.1%, respectively. Clonogenic activity indicated that the NBC fraction was highly enriched in hematopoietic progenitor cells (> 100%) as assessed in vitro (CFU-GM). Results were not affected by diagnosis, initial marrow volume or cell count of the BM suspension. We conclude that this is a simple and reproducible method using blood bank, facilities and permits BC preparation from BM without significant loss of hematopoietic progenitor cells.

[1]  O. Colvin,et al.  Autologous bone marrow transplantation in patients with acute nonlymphocytic leukemia, using ex vivo marrow treatment with 4-hydroperoxycyclophosphamide. , 1986, The New England journal of medicine.

[2]  E. Matutes,et al.  Purification of haemopoietic progenitor cells from patients with chronic granulocytic leukaemia using Percoll density gradients and elutriation , 1986, British journal of haematology.

[3]  C. Haanen,et al.  Enrichment of Human Bone Marrow Aspirates for Low‐Density Mononuclear Cells Using a Haemonetics Discontinuous Blood Cell Separator , 1986, Vox sanguinis.

[4]  J. Kersey,et al.  Transplantation of Major ABO‐Incompatible Bone Marrow Depleted of Red Cells by Hydroxyethyl Starch 1 , 1985, Vox sanguinis.

[5]  H. Martin,et al.  Gradient separation of granulocytic progenitor cells (CFUc) from human blood mononuclear leukocytes. , 1985, Experimental hematology.

[6]  B. Lamy,et al.  Removal of marrow T cells with OKT3-OKT11 monoclonal antibodies and complement to prevent acute graft-versus-host disease. A pilot study in ten patients. , 1985, Transplantation.

[7]  C. Haanen,et al.  BONE MARROW REPOPULATION CAPACITY AFTER TRANSPLANTATION OF LYMPHOCYTE‐DEPLETED ALLOGENEIC BONE MARROW USING COUNTERFLOW CENTRIFUGATION , 1984, Transplantation.

[8]  H. Prentice,et al.  A Technique for the Concentration of Nucleated Bone Marrow Cells for in vitro Manipulation or Cryopreservation Using the IBM 2991 Blood Cell Processor , 1983, Vox sanguinis.

[9]  P. Hervé,et al.  Méthode de concentration des cellules-souches médullaires utilisant le laveur IBM 2991Préalable essentiel avant traitement in vitro de la moelle osseuse par des moyens pharmacologiques ou immunologiques , 1983 .

[10]  D. Ma,et al.  Comparison of two methods for concentrating stem cells for cryopreservation and transplantation , 1982, Transfusion.

[11]  G. Janossy,et al.  A technique for rapid isolation of bone marrow mononuclear cells using Ficoll‐Metrizoate and the IBM 2991 blood cell processor , 1982, British journal of haematology.

[12]  H. Braine,et al.  Bone marrow transplantation with major ABO blood group incompatibility using erythrocyte depletion of marrow prior to infusion. , 1982, Blood.

[13]  W. Ross,et al.  Albumin density gradient purification of canine hemopoietic blood stem cells (HBSC): long-term allogeneic engraftment without GVH-reaction. , 1979, Experimental hematology.

[14]  M. Cline,et al.  A technique for the separation and cryopreservation of myeloid stem cells from human bone marrow. , 1979, Cryobiology.

[15]  R. Weiner,et al.  Semicontinuous flow centrifugation for the pheresis of immunocompetent cells and stem cells. , 1977, Blood.