A layout based customized testing technique for total microfluidic operations in digital microfluidic biochips

Digital microfluidic biochips in recent years have been developed as a major alternative platform for conventional benchtop laboratory procedures. It offers better precision, scalability, higher sensitivity, lower cost due to smaller sample and reagent volumes. Testing of DMFBs is of major significance in terms of dependability and reliability issues for safety-critical applications. A series of complex microfluidic operations are executed in a compact 2D array within a DMFB. The layout engages a group of cells as transportation path as well as a specific cluster of cells as functional modules to perform basic operations of routing, mixing, splitting, merging, storage and detection. In order to determine the correctness and reliability of results testing of these prescheduled layout are necessary both for transportation (structural) as well as functionality (functional). In this paper we propose a technique for customized testing of a prescheduled layout within a microfluidic array. The test performs both structural as well as functional testing for specified cells that forms the layout. The simulations are carried out in testbenches of benchmark suite III and the results are compared with contemporary methods.

[1]  Andrew Richardson,et al.  Built-in Test Solutions for the Electrode Structures in Bio-Fluidic Microsystems , 2009, 2009 14th IEEE European Test Symposium.

[2]  H. Rahaman,et al.  Accelerated Functional Testing of Digital Microfluidic Biochips , 2008, 2008 17th Asian Test Symposium.

[3]  Arun Ravindran,et al.  Efficient parallel testing and diagnosis of digital microfluidic biochips , 2009, JETC.

[4]  Sigurd Wagner,et al.  Thermocapillary actuation of droplets on chemically patterned surfaces by programmable microheater arrays , 2003 .

[5]  R. Fair,et al.  Electrowetting-based actuation of liquid droplets for microfluidic applications , 2000 .

[6]  Fei Su,et al.  Computer-Aided Design and Test for Digital Microfluidics , 2007, IEEE Design & Test of Computers.

[7]  Fei Su,et al.  Defect tolerance for gracefully-degradable microfluidics-based biochips , 2005, 23rd IEEE VLSI Test Symposium (VTS'05).

[8]  Krishnendu Chakrabarty,et al.  Digital Microfluidic Biochips - Design Automation and Optimization , 2010 .

[9]  Fei Su,et al.  Testing of droplet-based microelectrofluidic systems , 2003, International Test Conference, 2003. Proceedings. ITC 2003..

[10]  Fei Su,et al.  Test Planning and Test Resource Optimization for Droplet-Based Microfluidic Systems , 2004, Proceedings. Ninth IEEE European Test Symposium, 2004. ETS 2004..

[11]  K. Chakrabarty,et al.  Towards fault-tolerant digital microfluidic lab-on-chip: Defects, fault modeling, testing, and reconfiguration , 2008, 2008 IEEE Biomedical Circuits and Systems Conference.

[12]  Peter R. C. Gascoyne,et al.  Dielectrophoresis-based sample handling in general-purpose programmable diagnostic instruments , 2004, Proceedings of the IEEE.

[13]  Dave Hightower,et al.  A Generalized Channel Router , 1980, 17th Design Automation Conference.

[14]  Pranab Roy,et al.  A New Look Ahead Technique for Customized Testing in Digital Microfluidic Biochips , 2012, 2012 IEEE 21st Asian Test Symposium.

[15]  Krishnendu Chakrabarty,et al.  Functional testing of digital microfluidic biochips , 2007, 2007 IEEE International Test Conference.

[16]  Krishnendu Chakrabarty,et al.  Testing of Low-Cost Digital Microfluidic Biochips with Non-regular Array Layouts , 2010, Asian Test Symposium.

[17]  Ali Dolatabadi,et al.  A coupled electro-hydrodynamic numerical modeling of droplet actuation by electrowetting , 2008 .

[18]  Krishnendu Chakrabarty,et al.  Parallel Scan-Like Test and Multiple-Defect Diagnosis for Digital Microfluidic Biochips , 2007, IEEE Transactions on Biomedical Circuits and Systems.

[19]  Jean Berthier,et al.  Microdrops and digital microfluidics , 2008 .

[20]  Chang-Jin Kim,et al.  Addressable micro liquid handling by electric control of surface tension , 2001, Technical Digest. MEMS 2001. 14th IEEE International Conference on Micro Electro Mechanical Systems (Cat. No.01CH37090).

[21]  Jun Zeng,et al.  Principles of droplet electrohydrodynamics for lab-on-a-chip. , 2004, Lab on a chip.

[22]  Christian Druon,et al.  Plateforme saw dédiée à la microfluidique discrète pour applications biologiques , 2004 .

[23]  D. Davids,et al.  A fault detection and diagnosis technique for digital microfluidic biochips , 2008, 2008 IEEE 14th International Mixed-Signals, Sensors, and Systems Test Workshop.

[24]  Krishnendu Chakrabarty,et al.  Testing of Low-cost Digital Microfluidic Biochips with Non-Regular Array Layouts , 2010, 2010 19th IEEE Asian Test Symposium.

[25]  Fei Su,et al.  Concurrent testing of digital microfluidics-based biochips , 2006, TODE.

[26]  K. Chakrabarty,et al.  Ensuring the operational health of droplet-based microelectrofluidic biosensor systems , 2005, IEEE Sensors Journal.

[27]  Phil Paik,et al.  Rapid droplet mixers for digital microfluidic systems. , 2003, Lab on a chip.

[28]  Fei Su,et al.  Testing and Diagnosis of Realistic Defects in Digital Microfluidic Biochips , 2007, J. Electron. Test..

[29]  Krishnendu Chakrabarty,et al.  Built-in Self-Test and Fault Diagnosis for Lab-on-Chip Using Digital Microfluidic Logic Gates , 2008, 2008 IEEE International Test Conference.

[30]  Hans G. Kerkhoff.  Testing Microelectronic Biofluidic Systems , 2007, IEEE Design & Test of Computers.