Effect of UVA or UVB Irradiation on Cutaneous Lipids in Films or in Solution

The barrier function of the skin is largely due to the stratum corneum which is essentially composed of lipids. Different external factors, such as UV irradiation, affect this skin layer and are responsible for a destabilization of the supramolecular organization of its constituted lipids. In this work, mass spectrometry and infrared spectroscopy are combined to study the correlation between the formation of oxidative compounds by UV irradiation and the lipid organization. Experiments were carried out on unsaturated lipids in film or solution form, exposed to UVA or UVB irradiation. UV exposure leads to the formation of oxygenated entities in the case of lipids with an unsaturated fatty acid moiety, resulting in a decrease in their packing which is greater when the lipids are in solution. The packing decrease is even greater following UVB irradiation.

[1]  T. Shibamoto.  Analytical methods for trace levels of reactive carbonyl compounds formed in lipid peroxidation systems. , 2006, Journal of pharmaceutical and biomedical analysis.

[2]  H. Hirata,et al.  In vivo generation of free radicals in the skin of live mice under ultraviolet light, measured by L-band EPR spectroscopy. , 2006, Free radical biology & medicine.

[3]  H. Gardner.  Oxygen radical chemistry of polyunsaturated fatty acids. , 1989, Free radical biology & medicine.

[4]  M. Couturier,et al.  Determination of the yield of radiation-induced peroxidation of sodium linoleate in aqueous monomeric and micellar solutions. , 1998, Radiation research.

[5]  A. Girotti,et al.  Singlet Oxygen Adducts of Cholesterol: Photogeneration and Reductive Turnover in Membrane Systems , 1999, Photochemistry and photobiology.

[6]  A. Baillet,et al.  Role of ceramide structure and its microenvironment on the conformational order of model stratum corneum lipids mixtures: an approach by FTIR spectroscopy. , 2007, Chemistry and physics of lipids.

[7]  Jie Yao,et al.  Biophysical and morphological changes in the stratum corneum lipids induced by UVB irradiation. , 2006, Journal of dermatological science.

[8]  A. Baillet-Guffroy,et al.  Spectral monitoring of photoirradiated skin lipids: MS and IR approaches. , 2008, Chemistry and physics of lipids.

[9]  A. Weerheim,et al.  Determination of stratum corneum lipid profile by tape stripping in combination with high-performance thin-layer chromatography , 2001, Archives of Dermatological Research.

[10]  M. Plätzer,et al.  Mass Spectrometric Examinations of Stratum corneum Lipid Models Exposed to Ultraviolet Irradiation , 2003, Skin Pharmacology and Physiology.

[11]  A. Lavrijsen,et al.  Validation of an in vivo extraction method for human stratum corneum ceramides , 2004, Archives of Dermatological Research.

[12]  R. Mendelsohn,et al.  Infrared determination of conformational order and phase behavior in ceramides and stratum corneum models. , 2000, Methods in enzymology.

[13]  A. Baillet-Guffroy,et al.  Physical and chemical perturbations of the supramolecular organization of the stratum corneum lipids: in vitro to ex vivo study. , 2009, Biochimica et biophysica acta.

[14]  R. Mendelsohn,et al.  Determination of molecular conformation and permeation in skin via IR spectroscopy, microscopy, and imaging. , 2006, Biochimica et biophysica acta.

[15]  J. Fuchs,et al.  Measurements of UV-generated free radicals/reactive oxygen species (ROS) in skin. , 2006, Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy.

[16]  G. Buettner,et al.  Free radical-mediated lipid peroxidation in cells: oxidizability is a function of cell lipid bis-allylic hydrogen content. , 1993, Biochemistry.

[17]  J. Hadgraft,et al.  Topical delivery of caffeine from some commercial formulations. , 1999, International journal of pharmaceutics.

[18]  Mizushima,et al.  Intermolecular Interaction between a Synthetic Pseudoceramide and a Sterol-Combined Fatty Acid , 1997, Journal of colloid and interface science.

[19]  F. Rogge,et al.  Protein, lipid, and DNA radicals to measure skin UVA damage and modulation by melanin. , 2008, Free radical biology & medicine.

[20]  M Kawashima,et al.  Decreased level of ceramides in stratum corneum of atopic dermatitis: an etiologic factor in atopic dry skin? , 1991, The Journal of investigative dermatology.

[21]  S. Friberg,et al.  The effect of solvent extraction on the lipids of the stratum corneum in relation to observed immediate whitening of the skin , 1988, Contact dermatitis.

[22]  J. Bouwstra,et al.  The role of ceramide composition in the lipid organisation of the skin barrier. , 1999, Biochimica et biophysica acta.

[23]  J. Bouwstra,et al.  Modelling the stratum corneum lipid organisation with synthetic lipid mixtures: the importance of synthetic ceramide composition. , 2004, Biochimica et biophysica acta.

[24]  John Turk,et al.  Characterization of ceramides by low energy collisional-activated dissociation tandem mass spectrometry with negative-ion electrospray ionization , 2002, Journal of the American Society for Mass Spectrometry.

[25]  N. Yagi,et al.  Coexistence of two domains in intercellular lipid matrix of stratum corneum. , 2006, Biochimica et Biophysica Acta.