Porcine Circoviruses and Herpesviruses Are Prevalent in an Austrian Game Population

During the annual hunt in a privately owned Austrian game population in fall 2019 and 2020, 64 red deer (Cervus elaphus), 5 fallow deer (Dama dama), 6 mouflon (Ovis gmelini musimon), and 95 wild boars (Sus scrofa) were shot and sampled for PCR testing. Pools of spleen, lung, and tonsillar swabs were screened for specific nucleic acids of porcine circoviruses. Wild ruminants were additionally tested for herpesviruses and pestiviruses, and wild boars were screened for pseudorabies virus (PrV) and porcine lymphotropic herpesviruses (PLHV-1-3). PCV2 was detectable in 5% (3 of 64) of red deer and 75% (71 of 95) of wild boar samples. In addition, 24 wild boar samples (25%) but none of the ruminants tested positive for PCV3 specific nucleic acids. Herpesviruses were detected in 15 (20%) ruminant samples. Sequence analyses showed the closest relationships to fallow deer herpesvirus and elk gammaherpesvirus. In wild boars, PLHV-1 was detectable in 10 (11%), PLHV-2 in 44 (46%), and PLHV-3 in 66 (69%) of animals, including 36 double and 3 triple infections. No pestiviruses were detectable in any ruminant samples, and all wild boar samples were negative in PrV-PCR. Our data demonstrate a high prevalence of PCV2 and PLHVs in an Austrian game population, confirm the presence of PCV3 in Austrian wild boars, and indicate a low risk of spillover of notifiable animal diseases into the domestic animal population.

[1]  P. Ooi,et al.  What do we know about porcine Circovirus 3 (PCV3) diagnosis so far? (review). , 2021, Transboundary and emerging diseases.

[2]  C. Weissenbacher-Lang,et al.  Reconsideration of the diagnostic criteria required for PCV2 reproductive disease. , 2021, Veterinary journal.

[3]  G. Franzo,et al.  Porcine Gammaherpesviruses in Italian Commercial Swine Population: Frequent but Harmless , 2021, Pathogens.

[4]  J. Segalés,et al.  Lack of Porcine circovirus 4 Genome Detection in Pig Samples from Italy and Spain , 2020, Pathogens.

[5]  F. Conraths,et al.  Monitoring of Pseudorabies in Wild Boar of Germany—A Spatiotemporal Analysis , 2020, Pathogens.

[6]  L. Enquist,et al.  The Neuropathic Itch Caused by Pseudorabies Virus , 2020, Pathogens.

[7]  T. Opriessnig,et al.  Novel circovirus species identified in farmed pigs designated as Porcine circovirus 4, Hunan province, China. , 2019, Transboundary and emerging diseases.

[8]  S. Zhai,et al.  Reservoirs of Porcine Circoviruses: A Mini Review , 2019, Front. Vet. Sci..

[9]  Ting Ouyang,et al.  Recent progress on porcine circovirus type 3. , 2019, Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases.

[10]  G. Rákhely,et al.  Porcine circovirus type 3 detection in a Hungarian pig farm experiencing reproductive failures , 2019, Veterinary Record.

[11]  W. Fang,et al.  Prevalence of porcine circovirus type 3 in pigs in the southeastern Chinese province of Zhejiang , 2019, BMC Veterinary Research.

[12]  G. Franzo,et al.  A wild circulation: High presence of Porcine circovirus 3 in different mammalian wild hosts and ticks. , 2019, Transboundary and emerging diseases.

[13]  M. Stillfried,et al.  Detection of PCV3 in German wild boars , 2019, Virology Journal.

[14]  Yufeng Li,et al.  Detection and sequencing of porcine circovirus 3 in commercially sourced laboratory mice , 2019, Veterinary medicine and science.

[15]  Huijun Lu,et al.  An epidemiological investigation of porcine circovirus 3 infection in cattle in Shandong province, China , 2019, BMC Veterinary Research.

[16]  K. Schwartz,et al.  PCV3-associated disease in the United States swine herd , 2019, Emerging microbes & infections.

[17]  W. Wajjwalku,et al.  The retrospective identification and molecular epidemiology of porcine circovirus type 3 (PCV3) in swine in Thailand from 2006 to 2017 , 2018, Transboundary and emerging diseases.

[18]  J. Segalés,et al.  Porcine circovirus 3 is highly prevalent in serum and tissues and may persistently infect wild boar (Sus scrofa scrofa) , 2018, Transboundary and emerging diseases.

[19]  N. Vitale,et al.  Pseudorabies virus in North-West Italian wild boar (Sus scrofa) populations: prevalence and risk factors to support a territorial risk-based surveillance. , 2018, Veterinaria italiana.

[20]  Shanshan Zhu,et al.  Induction of Porcine Dermatitis and Nephropathy Syndrome in Piglets by Infection with Porcine Circovirus Type 3 , 2018, Journal of Virology.

[21]  Youzhi Li,et al.  Evidence of two genetically different lymphotropic herpesviruses present among red deer, sambar, and milu herds in China , 2018, Journal of veterinary science.

[22]  C. Sasakawa,et al.  First detection of porcine circovirus type 3 in Japan , 2018, The Journal of veterinary medical science.

[23]  G. Franzo,et al.  First report of wild boar susceptibility to Porcine circovirus type 3: High prevalence in the Colli Euganei Regional Park (Italy) in the absence of clinical signs , 2018, Transboundary and emerging diseases.

[24]  K. Alekseev,et al.  First detection and full genome sequence of porcine circovirus type 3 in Russia , 2018, Virus Genes.

[25]  J. Segalés,et al.  Full‐genome sequencing of porcine circovirus 3 field strains from Denmark, Italy and Spain demonstrates a high within‐Europe genetic heterogeneity , 2018, Transboundary and emerging diseases.

[26]  K. He,et al.  Phylogenetic analysis of two goat-origin PCV2 isolates in China. , 2018, Gene.

[27]  C. Fossum,et al.  Detection and genetic characterisation of porcine circovirus 3 from pigs in Sweden , 2018, Virus Genes.

[28]  R. Thanawongnuwech,et al.  Porcine circovirus type 3 (PCV3) infection in grower pigs from a Thai farm suffering from porcine respiratory disease complex (PRDC). , 2018, Veterinary microbiology.

[29]  F. Q. Mayer,et al.  Full‐Genome Sequence of Porcine Circovirus type 3 recovered from serum of sows with stillbirths in Brazil , 2018, Transboundary and emerging diseases.

[30]  H. Lu,et al.  The detection of porcine circovirus 3 in Guangxi, China , 2018, Transboundary and emerging diseases.

[31]  Xingxiao Zhang,et al.  Caprine herpesvirus 2-associated malignant catarrhal fever of captive sika deer (Cervus nippon) in an intensive management system , 2018, BMC Veterinary Research.

[32]  C. Sacchi,et al.  Detection and genetic characterization of Porcine circovirus type 3 in Italy , 2017, Transboundary and emerging diseases.

[33]  Dan Wang,et al.  Comparative epidemiology of porcine circovirus type 3 in pigs with different clinical presentations , 2017, Virology Journal.

[34]  K. Biernacka,et al.  First detection of porcine circovirus type 3 on commercial pig farms in Poland , 2017, Transboundary and emerging diseases.

[35]  Choi-Kyu Park,et al.  Prevalence of novel porcine circovirus 3 in Korean pig populations. , 2017, Veterinary microbiology.

[36]  P. Jiang,et al.  First molecular detection of porcine circovirus type 3 in dogs in China , 2017, Virus Genes.

[37]  E. Delwart,et al.  Erratum to: Detection of a novel circovirus PCV3 in pigs with cardiac and multi-systemic inflammation , 2017, Virology Journal.

[38]  R. Palinski,et al.  A Novel Porcine Circovirus Distantly Related to Known Circoviruses Is Associated with Porcine Dermatitis and Nephropathy Syndrome and Reproductive Failure , 2016, Journal of Virology.

[39]  S. Lavín,et al.  Pestivirus in alpine wild ruminants and sympatric livestock from the Cantabrian Mountains, Spain , 2016, Veterinary Record.

[40]  B. Martínez-López,et al.  Evidence of shared bovine viral diarrhea infections between red deer and extensively raised cattle in south-central Spain , 2016, BMC Veterinary Research.

[41]  F. Ruiz-Fons,et al.  A picture of trends in Aujeszky’s disease virus exposure in wild boar in the Swiss and European contexts , 2015, BMC Veterinary Research.

[42]  D. O'Toole,et al.  The Pathology of Malignant Catarrhal Fever, With an Emphasis on Ovine Herpesvirus 2 , 2014, Veterinary pathology.

[43]  K. Podgórska,et al.  Detection of porcine circovirus type 2 (PCV2) and porcine reproductive and respiratory syndrome virus (PRRSV) antibodies in meat juice samples from Polish wild boar (Sus scrofa L.). , 2013, Acta veterinaria Hungarica.

[44]  H. Stalder,et al.  Bovine viral diarrhea virus in free-ranging wild ruminants in Switzerland: low prevalence of infection despite regular interactions with domestic livestock , 2012, BMC Veterinary Research.

[45]  J. Segalés,et al.  Seroprevalence evolution of selected pathogens in Iberian wild boar. , 2012, Transboundary and emerging diseases.

[46]  S. Revilla-Fernández,et al.  Detection and molecular characterization of Suid herpesvirus type 1 in Austrian wild boar and hunting dogs. , 2012, Veterinary microbiology.

[47]  A. Palzer,et al.  PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS AND PORCINE CIRCOVIRUS TYPE 2 INFECTIONS IN WILD BOAR (SUS SCROFA) IN SOUTHWESTERN GERMANY , 2012, Journal of wildlife diseases.

[48]  E. C. Hahn,et al.  Pseudorabies virus in wild swine: a global perspective , 2011, Archives of Virology.

[49]  M. Spînu,et al.  Prevalence of porcine circoviruses in Transylvanian wild boars, detected by real-time PCR--short communication. , 2010, Acta veterinaria Hungarica.

[50]  K. Schoepf,et al.  Monitoring for Bovine Viral Diarrhea Virus in Austrian Red Deer (Cervus elaphus elaphus) by using Ear-notch Samples , 2010, Journal of wildlife diseases.

[51]  G. Reiner,et al.  Qualitative and quantitative distribution of PCV2 in wild boars and domestic pigs in Germany. , 2010, Veterinary microbiology.

[52]  Yeşilbağ Kadir,et al.  Genetic heterogeneity of bovine viral diarrhoea virus (BVDV) isolates from Turkey: identification of a new subgroup in BVDV-1. , 2008, Veterinary microbiology.

[53]  D. McGeoch,et al.  Novel Mammalian Herpesviruses and Lineages within the Gammaherpesvirinae: Cospeciation and Interspecies Transfer , 2008, Journal of Virology.

[54]  D. Walls,et al.  Infection of pigs in Ireland with lymphotropic gamma-herpesviruses and relationship to postweaning multisystemic wasting syndrome. , 2006, Veterinary microbiology.

[55]  I. Kiss,et al.  Genetic characterization of type 2 porcine circoviruses detected in Hungarian wild boars , 2006, Archives of Virology.

[56]  J. Cooley,et al.  A novel subgroup of rhadinoviruses in ruminants. , 2005, The Journal of general virology.

[57]  C. Chae,et al.  A review of porcine circovirus 2-associated syndromes and diseases. , 2005, Veterinary journal.

[58]  S. Nielsen,et al.  Pestivirus Exposure in Free-living and Captive Deer in Austria , 2004, Journal of wildlife diseases.

[59]  P. Hostnik,et al.  Phylogenetic analysis of type 2 porcine circoviruses identified in wild boar in Slovenia , 2004, Veterinary Record.

[60]  J. Segalés,et al.  Epidemiological study on porcine circovirus type 2 (PCV2) infection in the European wild boar (Sus scrofa). , 2004, Veterinary research.

[61]  J. Harding.  The clinical expression and emergence of porcine circovirus 2. , 2004, Veterinary microbiology.

[62]  J. Segalés,et al.  Pathological findings associated with naturally acquired porcine circovirus type 2 associated disease. , 2004, Veterinary microbiology.

[63]  B. Meehan,et al.  Porcine Circovirus 2–Associated Disease in Eurasian Wild Boar , 2003, Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc.

[64]  H. Ellerbrok,et al.  A novel porcine gammaherpesvirus. , 2003, Virology.

[65]  Mathias Ackermann,et al.  Porcines Bösartiges Katarrhalfieber: Diagnostische Befunde und erstmaliger Nachweis des Erregers bei erkrankten Schweinen in der Schweiz , 2003 .

[66]  Hong Li,et al.  Mural folliculitis and alopecia caused by infection with goat-associated malignant catarrhal fever virus in two sika deer. , 2002, Journal of the American Veterinary Medical Association.

[67]  N. Macintyre,et al.  Porcine dermatitis and nephropathy syndrome. , 2001, The Veterinary record.

[68]  B. Ehlers,et al.  Characterization of the DNA polymerase loci of the novel porcine lymphotropic herpesviruses 1 and 2 in domestic and feral pigs. , 1999, The Journal of general virology.

[69]  D. Haines,et al.  Reproduction of Lesions of Postweaning Multisystemic Wasting Syndrome in Gnotobiotic Piglets , 1999, Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc.

[70]  H. Reid,et al.  Malignant catarrhal fever caused by ovine herpesvirus-2 in pigs in Norway , 1998, Veterinary Record.

[71]  J. Segalés,et al.  Porcine dermatitis and nephropathy syndrome in Spain , 1998, Veterinary Record.

[72]  T. Rose,et al.  Detection and analysis of diverse herpesviral species by consensus primer PCR , 1996, Journal of clinical microbiology.

[73]  K. Lager,et al.  Effect of various vaccination procedures on shedding, latency, and reactivation of attenuated and virulent pseudorabies virus in swine. , 1992, American journal of veterinary research.

[74]  S. Odend'hal.  Malignant Catarrhal Fever Virus , 1983 .

[75]  H. Reid,et al.  An outbreak of malignant catarrhal fever in red deer (Cervus elephus) , 1979, Veterinary Record.

[76]  W. Plowright,et al.  Blue Wildebeest and the Ætiological Agent of Bovine Malignant Catarrhal Fever , 1960, Nature.