Production and immobilization of alkaline protease by Bacillus polymyxa which degrades various proteins

An aerobic gram positive spore forming bacterium, identified as Bacillus polymyxa was isolated from an alkaline soil. This isolated Bacillus produced maximum protease in milk at pH 7. The protease activity against casein was 580 Uml− 1 in milk, as the only source of carbon and energy. However, the maximum activity of protease on sorghums extracts, as the only carbon source was 525 Uml− 1. The best pH for enzyme production was 7, but the enzyme was active at pH 11. It was shown that the enzyme was stable at 55°C for 10 min. The imobilized enzyme with perlite was stable for more than 6 months. This enzyme hydrolyzed casein, gelatin and fibres. Hence it could be used in wastewater treatment, detergents and leathering.

[1]  E. Gormsen,et al.  Mechanistic studies of proteases and lipases for the detergent industry , 2007 .

[2]  R. Kothari,et al.  Isolation of Bacillus licheniformis mutants for stable production profiles of alkaline protease , 1986, Biotechnology Letters.

[3]  B. Gashe,et al.  Protease production by a thermophilic Bacillus species (P-001A) which degrades various kinds of fibrous proteins , 1993, Biotechnology Letters.

[4]  C. Wandrey,et al.  Production of alkaline protease with Bacillus licheniformis in a controlled fed-batch process , 1991, Applied Microbiology and Biotechnology.

[5]  C. Wandrey,et al.  Production of protease with Bacillus licheniformis mutants insensitive to repression of exoenzyme biosynthesis , 2004, Applied Microbiology and Biotechnology.

[6]  V. Punj,et al.  Isolation and partial characterization of a thermostable extracellular protease of Bacillus polymyxa B-17. , 1998, International journal of food microbiology.

[7]  B. K. Lonsane,et al.  Non-growth associated production of enzymes in solid state fermentation system: Its mathematical description for two enzymes produced by Bacillus licheniformis M27 , 1996 .

[8]  A. Jones,et al.  Purification and characterization of a protease from Pseudomonas pseudomallei. , 1994, Canadian journal of microbiology.

[9]  S. Braun,et al.  Enzymatic production of protein hydrolysates for food use , 1994 .

[10]  L. Mckay,et al.  Oversecretion of the Neutral Protease from Bacillus subtilis in Lactococcus lactis spp. lactis JF254 , 1994 .

[11]  S. Frokjaer.  Use of hydrolysates for protein supplementation , 1994 .

[12]  B. White,et al.  Enhancement and regulation of extracellular protein production by Bacillus brevis 47 through manipulation of cell culture conditions , 1992, Biotechnology and bioengineering.

[13]  H. Chang,et al.  Production of alkaline protease by Bacillus licheniformis in an aqueous two-phase system , 1990 .

[14]  R. E. Buchanan,et al.  Bergey's manual of determinative bacteriology. 8th Edition. , 1974 .

[15]  S. T. Cowan.  Bergey's Manual of Determinative Bacteriology , 1948, Nature.