Genetic regulation: the Lac control region.

The nucleotide sequence of the lac promoter-operator region has been determined. The 122 base pairs comprising this region include the recognition sites for RNA polymerase, the positive regulatory protein, CAP, and the negative regulatory protein, the repressor. Identification of mutant variants of the sequence combined with the in vitro biochemical studies of others has allowed us to tentatively identify the recognition site for each of these proteins, and to suggest how CAP might act at a distance to affect the interaction of RNA polymerase with the promoter.

[1]  J. Beckwith,et al.  Mutational alteration of the maximal level of Lac operon expression. , 1966, Cold Spring Harbor symposia on quantitative biology.

[2]  M. Schachner,et al.  On the Rule of Different Subunits of DNA-Dependent RNA Polymerase from E. coli in the Transcription Process , 1970 .

[3]  R F Newby,et al.  DNA binding of the lac repressor. , 1968, Journal of molecular biology.

[4]  J. Hopkins.  A new class of promoter mutations in the lactose operon of Escherichia coli. , 1974, Journal of molecular biology.

[5]  W. Gilbert,et al.  The nucleotide sequence of the lac operator. , 1973, Proceedings of the National Academy of Sciences of the United States of America.

[6]  B. Barrell,et al.  Bacteriophage φ80-induced low molecular weight RNA , 1972 .

[7]  A. Riggs,et al.  Purification and DNA-binding properties of the catabolite gene activator protein. , 1971, Proceedings of the National Academy of Sciences of the United States of America.

[8]  J. Beckwith,et al.  Cyclic Adenosine Monophosphate-Independent Mutants of the Lactose Operon of Escherichia coli , 1973, Journal of bacteriology.

[9]  J. Miller,et al.  The promoter-operator region of the lac operon of Escherichia coli. , 1968, Journal of molecular biology.

[10]  N. Maizels,et al.  The nucleotide sequence of the lactose messenger ribonucleic acid transcribed from the UV5 promoter mutant of Escherichia coli. , 1973, Proceedings of the National Academy of Sciences of the United States of America.

[11]  W. Reznikoff,et al.  The location of the repressor binding sites in the lac operon. , 1974, Proceedings of the National Academy of Sciences of the United States of America.

[12]  M J Chamberlin,et al.  The selectivity of transcription. , 1974, Annual review of biochemistry.

[13]  W. Gilbert,et al.  The lac operator is DNA. , 1967, Proceedings of the National Academy of Sciences of the United States of America.

[14]  W. Reznikoff,et al.  The operon revisited. , 1972, Annual review of genetics.

[15]  T. Ikemura,et al.  Small ribonucleic acids of Escherichia coli. I. Characterization by polyacrylamide gel electrophoresis and fingerprint analysis. , 1973, The Journal of biological chemistry.

[16]  R. Block,et al.  Mechanism of Initiation of Repression of In Vitro Transcription of the Lac Operon of Escherichia coli , 1971 .

[17]  J. Abelson,et al.  Nucleotide sequence determination of bacteriophage T4 leucine transfer ribonucleic acid. , 1973, The Journal of biological chemistry.

[18]  K. Beyreuther,et al.  The amino-acid sequence of lac repressor. , 1973, Proceedings of the National Academy of Sciences of the United States of America.

[19]  W. Reznikoff,et al.  Fusions of the lac and trp Regions of the Escherichia coli Chromosome , 1970, Journal of bacteriology.

[20]  W. Reznikoff,et al.  Inhibition of lacZ Gene Translation Initiation in trp-lac Fusion Strains , 1974, Journal of bacteriology.

[21]  M. Barkley,et al.  Measurements of unwinding of lac operator by repressor , 1974, Nature.

[22]  J. Beckwith,et al.  Evidence for two sites in the lac promoter region. , 1972, Journal of molecular biology.

[23]  E. Sutherland,et al.  ADENOSINE 3',5'-PHOSPHATE IN ESCHERICHIA COLI. , 1965, The Journal of biological chemistry.

[24]  A. Gierer.  Model for DNA and Protein Interactions and the Function of the Operator , 1966, Nature.

[25]  S. Weissman,et al.  Nucleotide sequence of a ribonucleic acid transcribed in vitro from lambda phage deoxyribonucleic acid. , 1971, The Journal of biological chemistry.

[26]  J. Monod,et al.  Genetic regulatory mechanisms in the synthesis of proteins. , 1961, Journal of molecular biology.