Synthesis of carbon‐14 labeled [1‐14C]‐, and [2‐14C]‐L‐tyrosine

Two labeled isotopomers of L-tyrosine, L-Tyr, have been synthesized using specific properties of the enzymes phenylanine ammonia lyase, PAL, and L-phenylalanine hydroxylase. In an intermediate step [1-14C]-, and [2-14C]-L-phenylalanine, L-Phe, have been obtained from [1-14C]-, and [2-14C]cinnamic acid, prepared from potassium [14C]cyanate or [2-14C]malonic acid, and by addition of ammonia in the presence of enzyme PAL. The labeled isotopomers of L-Phe have been converted into [1-14C]-, and [2-14C]-L-Tyr using the enzyme L-phenylalanine hydroxylase. Copyright © 2001 John Wiley & Sons, Ltd.

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