Can the black box be cracked? The augmentation of microbial ecology by high-resolution, automated sensing technologies

Automated sensing technologies, ‘ASTs,’ are tools that can monitor environmental or microbial-related variables at increasingly high temporal resolution. Microbial ecologists are poised to use AST data to couple microbial structure, function and associated environmental observations on temporal scales pertinent to microbial processes. In the context of aquatic microbiology, we discuss three applications of ASTs: windows on the microbial world, adaptive sampling and adaptive management. We challenge microbial ecologists to push AST potential in helping to reveal relationships between microbial structure and function.

[1]  L. Tranvik,et al.  � 2005, by the American Society of Limnology and Oceanography, Inc. Weak coupling between community composition and functioning of aquatic bacteria , 2022 .

[2]  A. Singh,et al.  Biodegradation and Bioremediation , 2020, Soil Biology.

[3]  H. Paerl Nuisance phytoplankton blooms in coastal, estuarine, and inland waters1 , 1988 .

[4]  Charles S. Yentsch,et al.  Anatomy of a red tide bloom off the southwest coast of Florida , 2008 .

[5]  M. Schlüter,et al.  Application of membrane inlet mass spectrometry for online and in situ analysis of methane in aquatic environments , 2008, Journal of the American Society for Mass Spectrometry.

[6]  Matthew C. Smith,et al.  A FIELD-ABLE RNA EXTRACTION AND PURIFICATION PROCEDURE , 2008 .

[7]  P. Arzberger,et al.  Sensors for Environmental Observatories , 2005 .

[8]  S. Benford,et al.  Real‐time physical data acquisition through a remote sensing platform on a polar lake , 2004 .

[9]  Timothy M. Shank,et al.  Use of voltammetric solid-state (micro)electrodes for studying biogeochemical processes: Laboratory measurements to real time measurements with an in situ electrochemical analyzer (ISEA) , 2008 .

[10]  Katherine D. McMahon,et al.  Typhoons initiate predictable change in aquatic bacterial communities , 2008 .

[11]  Matthew C. Smith,et al.  An integrated portable hand-held analyser for real-time isothermal nucleic acid amplification. , 2007, Analytica chimica acta.

[12]  P. Hanson,et al.  Wireless Sensor Networks for Ecology , 2005 .

[13]  M. Köster,et al.  Microbiosensors for Measurement of Microbially Available Dissolved Organic Carbon: Sensor Characteristics and Preliminary Environmental Application , 2006, Applied and Environmental Microbiology.

[14]  P. Hernández,et al.  An electronic tongue using potentiometric all-solid-state PVC-membrane sensors for the simultaneous quantification of ammonium and potassium ions in water , 2003, Analytical and bioanalytical chemistry.

[15]  Lora E Fleming,et al.  Literature Review of Florida Red Tide: Implications for Human Health Effects. , 2004, Harmful algae.

[16]  Paul C. Hanson A grassroots approach to sensor and science networks , 2007 .

[17]  Michael Wagner,et al.  Microbial community composition and function in wastewater treatment plants , 2002, Antonie van Leeuwenhoek.

[18]  R. Short,et al.  Detection and quantification of chemical plumes using a portable underwater membrane introduction mass spectrometer , 2006 .

[19]  J. Ingle,et al.  Monitoring Redox Conditions with Flow-Based and Fiber Optic Sensors Based on Redox Indicators: Application to Reductive Dehalogenation in a Bioaugmented Soil Column , 2007 .

[20]  Adriana Zingone,et al.  The diversity of harmful algal blooms: a challenge for science and management , 2000 .

[21]  J. Burkholder,et al.  Real-time remote monitoring of water quality: a review of current applications, and advancements in sensor, telemetry, and computing technologies , 2004 .

[22]  S. Chisholm,et al.  Measurement of Prochlorococcus ecotypes using real-time polymerase chain reaction reveals different abundances of genotypes with similar light physiologies. , 2006, Environmental microbiology.

[23]  Alisa Rudnitskaya,et al.  Sensor systems, electronic tongues and electronic noses, for the monitoring of biotechnological processes , 2008, Journal of Industrial Microbiology & Biotechnology.

[24]  J. Paul,et al.  Increased precision of microbial RNA quantification using NASBA with an internal control. , 2005, Journal of microbiological methods.

[25]  J. Paul,et al.  Development and evaluation of a method to detect and quantify enteroviruses using NASBA and internal control RNA (IC-NASBA). , 2005, Journal of Virological Methods.

[26]  N. Revsbech,et al.  Analysis of microbial communities with electrochemical microsensors and microscale biosensors. , 2005, Methods in enzymology.

[27]  Miguel Rodriguez,et al.  Biosensors for rapid monitoring of primary-source drinking water using naturally occurring photosynthesis. , 2002, Biosensors & bioelectronics.

[28]  Phil F. Culverhouse,et al.  Automatic image analysis of plankton: future perspectives , 2006 .

[29]  Gregory J. Doucette,et al.  Prospects for developing automated systems for in situ detection of harmful algae and their toxins , 2008 .

[30]  Henri Spanjers,et al.  Instrumentation in Anaerobic Treatment: Research and Practice , 2006 .

[31]  C. Gallegos,et al.  Temporal variability of optical properties in a shallow, eutrophic estuary: Seasonal and interannual variability , 2005 .

[32]  R. Benner Molecular Indicators of the Bioavailability of Dissolved Organic Matter , 2003 .

[33]  M P Sammartino,et al.  An algal biosensor for the monitoring of water toxicity in estuarine environments. , 2001, Water research.

[34]  M. Melkonian,et al.  Selective real-time herbicide monitoring by an array chip biosensor employing diverse microalgae , 2005, Journal of Applied Phycology.

[35]  Salvador Alegret,et al.  A flow-injection electronic tongue based on potentiometric sensors for the determination of nitrate in the presence of chloride , 2004 .

[36]  G. P. Kershaw,et al.  Soil microbial and nutrient dynamics in a wet Arctic sedge meadow in late winter and early spring , 2006 .

[37]  R. Rich,et al.  Survey of the year 2004 commercial optical biosensor literature , 2005, Journal of molecular recognition : JMR.

[38]  J. Munch,et al.  Harsh summer conditions caused structural and specific functional changes of microbial communities in an arable soil , 2007 .

[39]  J. Downing,et al.  The global abundance and size distribution of lakes, ponds, and impoundments , 2006 .

[40]  Peter Arzberger,et al.  New Eyes on the World: Advanced Sensors for Ecology , 2009 .

[41]  The NEREUS in‐lake wireless/acoustic chemical data network , 2008 .

[42]  David P. Fries,et al.  A handheld NASBA analyzer for the field detection and quantification of Karenia brevis , 2007 .

[43]  Laura E. Green,et al.  The role of ecological theory in microbial ecology , 2007, Nature Reviews Microbiology.

[44]  David N Lerner,et al.  Dissolved oxygen imaging in a porous medium to investigate biodegradation in a plume with limited electron acceptor supply. , 2003, Environmental science & technology.

[45]  M. Firestone,et al.  Linking microbial community composition to function in a tropical soil , 2000 .

[46]  Rebecca L Rich,et al.  Survey of the year 2007 commercial optical biosensor literature , 2008, Journal of molecular recognition : JMR.

[47]  Jason Feldman,et al.  Application of environmental sample processor (ESP) methodology for quantifying Pseudo‐nitzschia australis using ribosomal RNA‐targeted probes in sandwich and fluorescent in situ hybridization formats , 2006 .

[48]  J. Paul,et al.  Detection and Quantification of the Red Tide Dinoflagellate Karenia brevis by Real-Time Nucleic Acid Sequence-Based Amplification , 2004, Applied and Environmental Microbiology.

[49]  Mary Jane Perry,et al.  In Situ Instrumentation , 2007 .

[50]  Danielle R. Greenhow,et al.  High-resolution in situ analysis of nitrate and phosphate in the oligotrophic ocean. , 2007, Environmental science & technology.

[51]  E. Delong,et al.  Near real-time, autonomous detection of marine bacterioplankton on a coastal mooring in Monterey Bay, California, using rRNA-targeted DNA probes. , 2009, Environmental microbiology.