Detection of Bluetongue Virus Using a cDNA Probe Derived from Genome Segment 4 of Bluetongue Virus Serotype 2

The double-stranded (ds) RNA genome segment 4 of bluetongue virus (BTV) serotype 2 was cloned and used as a serogroup-specific complementary (c) DNA probe for BTV diagnosis. A cDNA representing a 60% copy of genome segment 4 of BTV-2 prototype was produced. The specificity of the cDNA probe was determined by hybridizing this probe to a northern blot of dsRNA (separated by polyacrylamide gel electrophoresis) of plaque-purified BTV-2 prototype. This cDNA probe was then used to hybridize to the RNA samples. Because the probe hybridized to all BTV samples but not to epizootic hemorrhagic disease virus samples, it appears to be a group-specific probe that could be used in BTV diagnosis.

[1]  J. Sambrook,et al.  Molecular Cloning: A Laboratory Manual , 2001 .

[2]  O. Kapp,et al.  Electron microscope studies , 1992 .

[3]  P. Roy,et al.  Detection of Bluetongue Virus RNA by in Situ Hybridization: Comparison with Virus Isolation and Antigen Detection , 1991, Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc.

[4]  W. Wilson,et al.  Limits of Detection of Bluetongue Virus with Different Assay Systems , 1990, Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc.

[5]  P. Roy,et al.  Completion of the sequence of bluetongue virus serotype 10 by the characterization of a structural protein, VP6, and a non-structural protein, NS2. , 1989, The Journal of general virology.

[6]  P. Roy.  Bluetongue virus genetics and genome structure. , 1989, Virus research.

[7]  R. Doi,et al.  Recombinant DNA probe for serotype-specific identification of bluetongue virus 17. , 1989, American journal of veterinary research.

[8]  R. Doi,et al.  Comparison of dot-blot and Northern blot hybridizations in the determination of genetic relatedness of United States bluetongue virus serotypes. , 1988, Journal of virological methods.

[9]  D. Ritter,et al.  Genetic relationships of bluetongue virus serotypes isolated from different parts of the world. , 1988, Virus research.

[10]  M. Cloete,et al.  The genetic relatedness of a number of individual cognate genes of viruses in the bluetongue and closely related serogroups. , 1987, Virology.

[11]  F. Brown,et al.  Assignment of the genome segments of bluetongue virus type 1 to the proteins which they encode. , 1984, Virology.

[12]  K. R. Squire,et al.  A survey of electropherotype relationships of bluetongue virus isolates from the western United States. , 1983, The Journal of general virology.

[13]  T. Close,et al.  Construction and characterization of the chloramphenicol-resistance gene cartridge: a new approach to the transcriptional mapping of extrachromosomal elements. , 1982, Gene.

[14]  P. C. Lee,et al.  Cloning the double-stranded RNA genes of reovirus: sequence of the cloned S2 gene. , 1982, Proceedings of the National Academy of Sciences of the United States of America.

[15]  B. M. Gorman,et al.  Comparison of bluetongue type 20 with certain viruses of the bluetongue and Eubenangee serological groups of orbiviruses. , 1981, The Journal of general virology.

[16]  J. Stott,et al.  Epizootiologic study of bluetongue: virologic and serologic results. , 1981, American journal of veterinary research.

[17]  M. McCrae,et al.  A rapid and sensitive method for analysing the genome profiles of field isolates of rotavirus. , 1981, Journal of virological methods.

[18]  E. Borden,et al.  Physicochemical and morphological relationships of some arthropod-borne viruses to bluetongue virus--a new taxonomic group. Electron microscopic studies. , 1971, The Journal of general virology.

[19]  H. Els,et al.  Morphology of bluetongue virus. , 1969, Virology.

[20]  J. Stott,et al.  Application of molecular techniques to the diagnosis of bluetongue virus infection. , 1987, Progress in veterinary microbiology and immunology.

[21]  R. Shope,et al.  Overview of the orbiviruses. , 1985, Progress in clinical and biological research.

[22]  Shope Re,et al.  Overview of the orbiviruses. , 1985 .