Induction of Triploid Abalone, Haliotis discus hannai, and Its Biological Characteristics I. Induction of Triploid Abalone

Triploid abalone, Haliotis discus hannai, was induced by the cold or the heat shock procedure with fertilized eggs, 12 min. or 32 min. post fertilization with the various time intervals of shock duration. Fertilization rate of each experimental group was not significantly different from that of corresponding diploid control (P>0.05). However hatching rates and normality rates of triploid larvae were significantly different from those of corresponding diploid control (P , fertilization rate of eggs was extremely low and hatched larvae were not detected in these treatment groups. Incidence of triploidy was confirmed by chromosome count and the highest rates of triploid revealed in cold shock with 15 min. treatment duration 12 min. after fertilization. The number of diploid chromosome was 2n: 36, and that of tiploid was 3n=54.