Luminol chemiluminescence biosensor for glycated hemoglobin (HbA1c) in human blood samples.

Luminol chemiluminescence (CL) biosensor based on boronic acid modified gold substrate has been developed for the determination of glycated hemoglobin (HbA1c) in human blood samples. In order to selectively capture HbA1c in sample, carboxy-EG6-undecanethiol was self-assembled on a gold thin-film substrate, followed by covalent coupling of 3-aminophenyl boronic acid (3-APBA). The captured HbA1c containing four iron heme groups plays as a catalyst for luminol CL reaction in the presence of hydrogen peroxide, and thus the luminol CL response is linearly proportional to the amount of HbA1c captured on the biosensor surface. The present biosensor showed linear dynamic range of HbA1c from 2.5% to 17.0%, which well covers the clinically important concentration range. In addition, the present biosensor exhibited negligible response to interfering species such as hemoglobin, fructose, and sorbitol. The present HbA1c biosensor was applied to the determination of HbA1c in human blood samples and the results were well agreed with that obtained with a conventional method.

[1]  J. Jeppsson,et al.  Improved Method for Analysis of Glycated Haemoglobin by Ion Exchange Chromatography , 1994, Annals of clinical biochemistry.

[2]  A. Knight,et al.  Occurrence, mechanisms and analytical applications of electrogenerated chemiluminescence. A review , 1994 .

[3]  Shanhong Xia,et al.  A micro-potentiometric hemoglobin immunosensor based on electropolymerized polypyrrole-gold nanoparticles composite. , 2009, Biosensors & bioelectronics.

[4]  P. Gallop,et al.  The biosynthesis of human hemoglobin A1c. Slow glycosylation of hemoglobin in vivo. , 1976, The Journal of clinical investigation.

[5]  Yoon-Bo Shim,et al.  Disposable amperometric glycated hemoglobin sensor for the finger prick blood test. , 2013, Analytical chemistry.

[6]  Adsorption of proteins of the surfaces of modified silicas , 1987 .

[7]  D. Wayner,et al.  REDOX SWITCHING OF CARBOHYDRATE BINDING TO FERROCENE BORONIC ACID , 1999 .

[8]  Wen-Yih Chen,et al.  The investigation of recognition interaction between phenylboronate monolayer and glycated hemoglobin using surface plasmon resonance. , 2008, Analytical biochemistry.

[9]  G. Sauerbrey,et al.  Use of quartz vibration for weighing thin films on a microbalance , 1959 .

[10]  Chandra Shekhar Pundir,et al.  Determination of glycated hemoglobin with special emphasis on biosensing methods. , 2014, Analytical biochemistry.

[11]  Wei Wang,et al.  Ag nanoparticle-catalyzed chemiluminescent reaction between luminol and hydrogen peroxide , 2008 .

[12]  J. Lafferty,et al.  Evaluation of a dual hemoglobin A(2)/A(1c) quantitation kit on the bio-rad variant II automated hemoglobin analyzer. , 2009, Archives of pathology & laboratory medicine.

[13]  J. Ladenson,et al.  Glycated hemoglobin and diabetes: a case and an overview of the subject. , 1985, Clinical chemistry.

[14]  Caifeng Ding,et al.  Ultrasensitive flow injection chemiluminescence detection of DNA hybridization using signal DNA probe modified with Au and CuS nanoparticles. , 2008, Analytical chemistry.

[15]  Hua Cui,et al.  Gold nanoparticle-catalyzed luminol chemiluminescence and its analytical applications. , 2005, Analytical chemistry.

[16]  Zhu Chang,et al.  Fabrication of electrochemical interface based on boronic acid-modified pyrroloquinoline quinine/reduced graphene oxide composites for voltammetric determination of glycated hemoglobin. , 2015, Biosensors & bioelectronics.

[17]  Václav Kašička,et al.  Analysis of glycated hemoglobin A1c by capillary electrophoresis and capillary isoelectric focusing. , 2011, Analytical biochemistry.

[18]  G. Sauerbrey.  Verwendung von Schwingquarzen zur Wägung dünner Schichten und zur Mikrowägung , 1959 .

[19]  Hyun C. Yoon,et al.  Boronic acid-modified thin film interface for specific binding of glycated hemoglobin (HbA1c) and electrochemical biosensing , 2009 .

[20]  D. Nathan,et al.  Immediate feedback of HbA1c levels improves glycemic control in type 1 and insulin-treated type 2 diabetic patients. , 1999, Diabetes care.

[21]  T Tanaka,et al.  Detection of HbA(1c) by boronate affinity immunoassay using bacterial magnetic particles. , 2001, Biosensors & bioelectronics.

[22]  Jan Přibyl,et al.  Quartz crystal biosensor for detection of sugars and glycated hemoglobin , 2005 .

[23]  R. Dasari,et al.  Raman spectroscopy-based sensitive and specific detection of glycated hemoglobin. , 2012, Analytical chemistry.

[24]  G. Absalan,et al.  Effect of gold nanoparticle as a novel nanocatalyst on luminol-hydrazine chemiluminescence system and its analytical application. , 2008, Analytica chimica acta.

[25]  Frieder W. Scheller,et al.  Ferroceneboronic acid-based amperometric biosensor for glycated hemoglobin , 2006 .

[26]  H. Cui,et al.  Comparative studies on electrogenerated chemiluminescence of luminol on gold nanoparticle modified electrodes. , 2007, Langmuir : the ACS journal of surfaces and colloids.

[27]  F. Frantzen,et al.  Soluble highly coloured phenylboronic acids and their use in glycohemoglobin quantification. , 1997, Clinica chimica acta; international journal of clinical chemistry.

[28]  Byoung-Yong Chang,et al.  Selective electrochemical sensing of glycated hemoglobin (HbA1c) on thiophene-3-boronic acid self-assembled monolayer covered gold electrodes. , 2008, Analytical chemistry.

[29]  Petr Skládal,et al.  Development of a combined setup for simultaneous detection of total and glycated haemoglobin content in blood samples. , 2006, Biosensors & bioelectronics.

[30]  Bo Mattiasson,et al.  Application of shielding boronate affinity chromatography in the study of the glycation pattern of haemoglobin. , 2002, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[31]  A. Berti,et al.  Forensic application of the luminol reaction as a presumptive test for latent blood detection. , 2007, Talanta.

[32]  Frieder W. Scheller,et al.  Membrane-immobilized haptoglobin as affinity matrix for a hemoglobin-A1c immunosensor , 2002 .