“FISHing” to detect urinary and other cancers

The UroVysion Bladder Cancer Kit detects amplifications of chromosomes 3, 7, and 17, and the deletion of 9p21, by fluorescence in situ hybridization (FISH). Because manual interpretation of UroVysion FISH is time consuming and can be challenged by variable probe signal strengths and background labeling, the authors investigated an automated image analysis system to improve throughput, productivity, quality control, and accuracy.

[1]  Liron Pantanowitz,et al.  The impact of digital imaging in the field of cytopathology , 2009, CytoJournal.

[2]  H. Grossman,et al.  Combined morphologic and fluorescence in situ hybridization analysis of voided urine samples for the detection and follow-up of bladder cancer in patients with benign urine cytology , 2008 .

[3]  I. Leibovitch,et al.  Combined morphologic and fluorescence in situ hybridization analysis of voided urine samples for the detection and follow‐up of bladder cancer in patients with benign urine cytology , 2007, Cancer.

[4]  Tullio Sulser,et al.  Multi‐target fluorescence in situ hybridization in bladder washings for prediction of recurrent bladder cancer , 2006, International journal of cancer.

[5]  K. Halling,et al.  An evaluation of ThinPrep UroCyte filters for the preparation of slides for fluorescence in situ hybridization , 2006, Diagnostic cytopathology.

[6]  I. Leibovitch,et al.  Combined analysis of morphology and fluorescence in situ hybridization significantly increases accuracy of bladder cancer detection in voided urine samples. , 2005, Urology.

[7]  J. Jones,et al.  Multitarget fluorescence in situ hybridization assay detects transitional cell carcinoma in the majority of patients with bladder cancer and atypical or negative urine cytology. , 2003, The Journal of urology.

[8]  L. Bubendorf,et al.  Multiprobe FISH for enhanced detection of bladder cancer in voided urine specimens and bladder washings. , 2001, American journal of clinical pathology.

[9]  R. Jenkins,et al.  The development of a multitarget, multicolor fluorescence in situ hybridization assay for the detection of urothelial carcinoma in urine. , 2000, The Journal of molecular diagnostics : JMD.

[10]  Y. Miyaji,et al.  Chromosomal numerical aberrations of exfoliated cells in the urine detected by fluorescence in situ hybridization: clinical implication for the detection of bladder cancer , 2000, Urological Research.

[11]  R. Hoda The Art and Science of Cytopathology , 1996 .

[12]  A. Thor,et al.  The art and science of cytopathology: Richard M. DeMay, MD. Chicago IL, ASCP Press, 1996, 2 vol set, 1,289 pages, $285 , 1996 .