Storage-induced denitrification using sequencing batch reactor operation

Abstract As a biochemical reduction of oxidized-nitrogen species, the denitrification reaction requires the availability of an electron-donor substrate. This requirement is typically satisfied by the provision of an organic carbon supplement or, possibly, a reactor design which utilizes raw-waste organic carbon components. Bacterial storage can also provide the necessary electron source. However, in the instance of conventional wastewater treatment facilities, the limited availability of such cellular reserve material results in a markedly reduced denitrification capacity. Bench-scale studies of storage induced denitrification were conducted using a sequencing batch reactor. By operating the reactor in a mode conducive to the development of cellular storage, the system maintained a consistent 92+% reduction in total nitrogen without a carbon supplement. Depletion of the cellular glycogen reserve was observed during the denitrification reaction.