Novel candidate tumor marker genes for lung adenocarcinoma

Using the representational difference analysis (RDA) technique on pooled mRNA of five primary lung adenocarcinomas and their corresponding non-neoplastic lung tissues, we identified six genes that were putatively overexpressed in this type of lung cancer. Five corresponded to previously isolated genes, while one (Lc19) matched with the sequence of an unannotated EST. Real-time RT–PCR analyses of expression levels in a panel of 34 paired primary non-small cell lung cancer (NSCLC) and corresponding grossly normal appearing lung tissues confirmed the common overexpression of these genes in non-small cell lung cancer. Among these genes, overexpression of Lc19, hyaluronan binding protein 2 (HABP2) and crystalline-mu appeared more specific to adenocarcinoma, whereas ceruloplasmin, integrin α-11 and collagen type XI alpha 1 were overexpressed at high frequency among both adenocarcinoma and squamous cell carcinoma. These genes represent novel candidate tumor biomarker genes for NSCLC and its histological subtypes.

[1]  G. G. Stokes.  "J." , 1890, The New Yale Book of Quotations.

[2]  P. Terasaki,et al.  Detection of tumor-associated antigens in the sera of lung cancer patients by three monoclonal antibodies. , 1985, Cancer research.

[3]  M. Tockman,et al.  Sensitive and specific monoclonal antibody recognition of human lung cancer antigen on preserved sputum cells: a new approach to early lung cancer detection. , 1988, Journal of clinical oncology : official journal of the American Society of Clinical Oncology.

[4]  S. Antonarakis,et al.  Localization of the human gene for mu-crystallin to chromosome 16p. , 1992, Genomics.

[5]  M. Wigler,et al.  Cloning the differences between two complex genomes , 1993, Science.

[6]  M Hubank,et al.  Identifying differences in mRNA expression by representational difference analysis of cDNA. , 1994, Nucleic acids research.

[7]  R. Hruban,et al.  Detection of oncogene mutations in sputum precedes diagnosis of lung cancer. , 1994, Cancer research.

[8]  P. Schraml,et al.  Identification of genes differentially expressed in normal lung and non-small cell lung carcinoma tissue. , 1994, Cancer research.

[9]  C. Denny,et al.  Identification of target genes for the Ewing's sarcoma EWS/FLI fusion protein by representational difference analysis , 1995, Molecular and cellular biology.

[10]  T. Soussi,et al.  Serum p53 antibodies as early markers of lung cancer , 1995, Nature Medicine.

[11]  T. Tobe,et al.  Purification and characterization of a novel hyaluronan-binding protein (PHBP) from human plasma: it has three EGF, a kringle and a serine protease domain, similar to hepatocyte growth factor activator. , 1996, Journal of biochemistry.

[12]  N. Shimizu,et al.  Isolation and characterization of the plasma hyaluronan-binding protein (PHBP) gene (HABP2). , 1997, Journal of biochemistry.

[13]  C. Mountain,et al.  Revisions in the International System for Staging Lung Cancer. , 1997, Chest.

[14]  M. Thun,et al.  Cigarette smoking and changes in the histopathology of lung cancer. , 1997, Journal of the National Cancer Institute.

[15]  M. Meisler,et al.  Marshall syndrome associated with a splicing defect at the COL11A1 locus. , 1998, American journal of human genetics.

[16]  S. Nelson,et al.  Detection of differentially expressed genes in primary tumor tissues using representational differences analysis coupled to microarray hybridization. , 1998, Nucleic acids research.

[17]  T. Sejersen,et al.  cDNA cloning and chromosomal localization of human alpha(11) integrin. A collagen-binding, I domain-containing, beta(1)-associated integrin alpha-chain present in muscle tissues. , 1999, The Journal of biological chemistry.

[18]  C. Morris,et al.  Cloning, sequence analysis, and chromosomal localization of the novel human integrin α11 subunit (ITGA11) , 1999 .

[19]  J. Yokota,et al.  Identification of genes (SPON2 and C20orf2) differentially expressed between cancerous and noncancerous lung cells by mRNA differential display. , 1999, Genomics.

[20]  A. Kraus,et al.  Differential frequencies of p16(INK4a) promoter hypermethylation, p53 mutation, and K-ras mutation in exfoliative material mark the development of lung cancer in symptomatic chronic smokers. , 2000, Journal of clinical oncology : official journal of the American Society of Clinical Oncology.

[21]  Cheryl Schmidt,et al.  Identification of genes differentially over-expressed in lung squamous cell carcinoma using combination of cDNA subtraction and microarray analysis , 2000, Oncogene.

[22]  T. Lee,et al.  Molecular and genetic aspects of lung cancer. , 2000, American journal of respiratory and critical care medicine.

[23]  C. Byus,et al.  Optimization of cDNA representational difference analysis for the identification of differentially expressed mRNAs. , 2000, Analytical biochemistry.

[24]  P. Maloney,et al.  Cancer-specific genomic instability in bronchial lavage: a molecular tool for lung cancer detection. , 2001, Cancer research.

[25]  F. Fougerousse,et al.  α11β1 Integrin Is a Receptor for Interstitial Collagens Involved in Cell Migration and Collagen Reorganization on Mesenchymal Nonmuscle Cells , 2001 .

[26]  D. Botstein,et al.  Diversity of gene expression in adenocarcinoma of the lung , 2001, Proceedings of the National Academy of Sciences of the United States of America.

[27]  A. Tanigami,et al.  Isolation of a novel human lung‐specific gene, LUNX, a potential molecular marker for detection of micrometastasis in non‐small‐cell lung cancer , 2001, International journal of cancer.

[28]  Taylor Murray,et al.  Cancer Statistics, 2001 , 2001, CA: a cancer journal for clinicians.

[29]  E. Lander,et al.  Classification of human lung carcinomas by mRNA expression profiling reveals distinct adenocarcinoma subclasses , 2001, Proceedings of the National Academy of Sciences of the United States of America.

[30]  Tzei-Yi Lin,et al.  Overexpression of dihydrodiol dehydrogenase as a prognostic marker of non-small cell lung cancer. , 2001, Cancer research.