论文信息 - A simple and modified manometric method for measuring oxygen uptake rate of plant cells in flask cultures

A simple and modified manometric method for measuring oxygen uptake rate of plant cells in flask cultures

A simple and convenient technique was developed based on the principle of Warburg manometric method to measure O2 uptake rate (OUR) and CO2 evolution rate (CER) of suspended cells in a shake flask culture. It was successfully applied to suspension cultures of rice (Oryza sativa) and Panax notoginseng cells, and some important bioprocess parameters, such as OUR, CER, respiratory quotient (RQ), specific OUR (SOUR) and specific CER (SCER), were quantitatively obtained. The measuring system is easy to operate, able to treat many samples simultaneously and is economical.

Jian-Jiang Zhong | Z. Wen | J. Zhong | Zhi-You Wen

[1] R. Lovrien. Manometric and Biochemical Techniques , 1973 .

[2] F. Skoog,et al. A revised medium for rapid growth and bio assays with tobacco tissue cultures , 1962 .

[3] T Seki,et al. On-line monitoring of cell concentration of Perilla frutescens in a bioreactor. , 1993, Biotechnology and bioengineering.

[4] C. Chu,et al. ESTABLISHMENT OF AN EFFICIENT MEDIUM FOR ANTHER CULTURE OF RICE THROUGH COMPARATIVE EXPERIMENTS ON THE NITROGEN SOURCES , 1975 .

[5] Jian-Jiang Zhong,et al. Computer-aided on-line monitoring of physiological variables in suspended cell cultures of Perilla frutescens in a bioreactor , 1994 .