On-chip immuno-agglutination assay with analyte capture by dynamic manipulation of superparamagnetic beads.

Magnetic bead-based lab-on-a-chip systems offer significant advantages compared to more conventional systems, mainly through the possibility of controlled manipulation of the magnetic carriers on-chip. In particular, microfluidic immunoassays using functionalized magnetic beads raise increasing interest. We present here a new approach for performing immuno-agglutination assays on-chip. Our system is based on a quadrupolar magnetic field set-up. Dynamic actuation of a confined plug of functionalized magnetic beads is used for analyte capture in a microchannel. A simple detection method based on the swelling of the released plug after agglutination is presented. We demonstrate the feasibility of on-chip agglutination tests by means of a streptavidin/biotinylated-bovine serum albumin (bBSA) model assay. A detection limit of about 200 pg/mL (approximately 3 pM) is achieved.

[1]  J. Baudry,et al.  TOPICAL REVIEW: Bio-specific recognition and applications: from molecular to colloidal scales , 2004 .

[2]  C T Lim,et al.  Bead-based microfluidic immunoassays: the next generation. , 2007, Biosensors & bioelectronics.

[3]  C. Bárcena,et al.  APPLICATIONS OF MAGNETIC NANOPARTICLES IN BIOMEDICINE , 2003 .

[4]  Andreas Dietzel,et al.  Transient behaviour of magnetic micro-bead chains rotating in a fluid by external fields. , 2007, Lab on a chip.

[5]  A. Gast,et al.  Micromixing with linked chains of paramagnetic particles. , 2004, Analytical chemistry.

[6]  Thermal expansion within a chain of magnetic colloidal particles. , 2009, Physical review. E, Statistical, nonlinear, and soft matter physics.

[7]  Elisabeth Verpoorte,et al.  Beads and chips: new recipes for analysis. , 2003, Lab on a chip.

[8]  Q. Pankhurst,et al.  Applications of magnetic nanoparticles in biomedicine , 2003 .

[9]  Jaap M J den Toonder,et al.  Chaotic mixing induced by a magnetic chain in a rotating magnetic field. , 2007, Physical review. E, Statistical, nonlinear, and soft matter physics.

[10]  Jaephil Do,et al.  A polymer lab-on-a-chip for magnetic immunoassay with on-chip sampling and detection capabilities. , 2008, Lab on a chip.

[11]  Mikkel Fougt Hansen,et al.  Theoretical comparison of magnetic and hydrodynamic interactions between magnetically tagged particles in microfluidic systems , 2005 .

[12]  Nicole Pamme,et al.  Simultaneous bioassays in a microfluidic channel on plugs of different magnetic particles. , 2008, Analytica chimica acta.

[13]  P Tabeling,et al.  Improving agglutination tests by working in microfluidic channels. , 2005, Lab on a chip.

[14]  C. Plotz,et al.  The latex fixation test. I. Application to the serologic diagnosis of rheumatoid arthritis. , 1956, The American journal of medicine.

[15]  Martin A M Gijs,et al.  Full on-chip nanoliter immunoassay by geometrical magnetic trapping of nanoparticle chains. , 2008, Analytical chemistry.

[16]  Martin A. M. Gijs,et al.  Quadrupolar magnetic actuation of superparamagnetic particles for enhanced microfluidic perfusion , 2009 .

[17]  Nicole Pamme,et al.  Magnetism and microfluidics. , 2006, Lab on a chip.

[18]  Martin A. M. Gijs,et al.  Magnetic bead handling on-chip: new opportunities for analytical applications , 2004 .

[19]  Jean-Louis Viovy,et al.  Controlled proteolysis of normal and pathological prion protein in a microfluidic chip. , 2008, Lab on a chip.

[20]  C. Robic,et al.  Acceleration of the recognition rate between grafted ligands and receptors with magnetic forces , 2006, Proceedings of the National Academy of Sciences.

[21]  Marc Herrmann,et al.  Microfluidic ELISA on non-passivated PDMS chip using magnetic bead transfer inside dual networks of channels. , 2007, Lab on a chip.

[22]  Henrik Bruus,et al.  Microfluidic capturing-dynamics of paramagnetic bead suspensions. , 2005, Lab on a chip.

[23]  Amar Rida,et al.  Dynamics of magnetically retained supraparticle structures in a liquid flow , 2004 .