Novel Microscope-Based Approaches for the Investigation of Protein-Protein Interactions in Signal Transduction

In addition to the biochemical characterization of interacting proteins, the spatiotemporal localisation in situ or in vivo of the transiently associating participants of a cascade mechanism can greatly enhance our understanding of the given signal transduction process. However, the mere determination of colocalization based on fluorescent tags that are compatible with the in vivo observation does not generally suffice due to the limitation in resolution imposed by optical diffraction in conventional light microscopy.

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