Fast and Efficient Nucleic Acid Testing by ST's In-Check TM Lab-on-Chip Platform

In this contribution we report Nucleic Acid (NA) analyses by using the ST’s In-Check™ lab-on-chip Platform. Such a platform is based on a miniaturized silicon Lab-on-Cchip (LoC) that integrates a Polymerase Chain Reaction (PCR) reactor - formed by buried microchannels 1 ul in volume – together with a customable microarray. Temperature Control System (TCS) and Optical Reader (OR) appliances, driven by a tailored bioinformatic system, complete the platform. These features allow achieving both DNA amplification and NA detection in an integrated cost effective and convenient format silicon chip. Integrated detection (PCR and microarray) along with the reproducibility and sensitivity study of integrated PCR by using betaglobin gene from human genomic DNA in ST’s In-Check™ platform are presented and discussed.