Differential effect of oleic and palmitic acid on lipid accumulation and apoptosis in cultured hepatocytes

Background and Aim:  Studies have shown monounsaturated oleic acid to be less toxic than palmitic acid and to prevent/attenuate palmitic acid hepatocites toxicity in steatosis models in vitro. However, to what degree these effects are mediated by steatosis extent is unknown.

[1]  José Vicente Castell,et al.  A human hepatocellular in vitro model to investigate steatosis. , 2007, Chemico-biological interactions.

[2]  G. Gores,et al.  The Relationship Between Apoptosis and Non-Alcoholic Fatty Liver Disease: An Evolutionary Cornerstone Turned Pathogenic , 2005, Zeitschrift fur Gastroenterologie.

[3]  M. Karin,et al.  Saturated fatty acids inhibit induction of insulin gene transcription by JNK-mediated phosphorylation of insulin-receptor substrates , 2006, Proceedings of the National Academy of Sciences.

[4]  J. Horton,et al.  Molecular mediators of hepatic steatosis and liver injury. , 2004, The Journal of clinical investigation.

[5]  S. Banni,et al.  Characterization of conjugated linoleic acid and its metabolites by RP‐HPLC with diode array detector , 2001 .

[6]  G. Gores,et al.  O0040 FREE FATTY ACIDS PROMOTE HEPATIC LIPOTOXICITY BY STIMULATING TNF‐ALPHA EXPRESSION VIA A LYSOSOMAL PATHWAY , 2004, Journal of Pediatric Gastroenterology and Nutrition.

[7]  I. T. de Almeida,et al.  Plasma total and free fatty acids composition in human non-alcoholic steatohepatitis. , 2002, Clinical nutrition.

[8]  N. Javitt Hep G2 cells as a resource for metabolic studies: lipoprotein, cholesterol, and bile acids , 1990, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[9]  M. Pagliassotti,et al.  Saturated fatty acids promote endoplasmic reticulum stress and liver injury in rats with hepatic steatosis. , 2006, Endocrinology.

[10]  D. Eizirik,et al.  Inverse relationship between cytotoxicity of free fatty acids in pancreatic islet cells and cellular triglyceride accumulation. , 2001, Diabetes.

[11]  M. Pagliassotti,et al.  The role of fatty acids in the development and progression of nonalcoholic fatty liver disease. , 2008, The Journal of nutritional biochemistry.

[12]  A. Lonardo,et al.  Hepatic steatosis and insulin resistance: does etiology make a difference? , 2006, Journal of hepatology.

[13]  G. Gores,et al.  Hepatocyte apoptosis and fas expression are prominent features of human nonalcoholic steatohepatitis. , 2003, Gastroenterology.

[14]  G. Gores,et al.  Hepatocyte apoptosis is a pathologic feature of human alcoholic hepatitis. , 2001, Journal of hepatology.

[15]  J. Jessurun,et al.  Sources of fatty acids stored in liver and secreted via lipoproteins in patients with nonalcoholic fatty liver disease. , 2005, The Journal of clinical investigation.

[16]  G. Gores,et al.  The lysosomal‐mitochondrial axis in free fatty acid–induced hepatic lipotoxicity , 2008, Hepatology.

[17]  D. Befroy,et al.  Mechanism of Hepatic Insulin Resistance in Non-alcoholic Fatty Liver Disease* , 2004, Journal of Biological Chemistry.

[18]  N. Tennagels,et al.  Adiponectin counteracts cytokine- and fatty acid-induced apoptosis in the pancreatic beta-cell line INS-1 , 2004, Diabetologia.

[19]  G. Gores,et al.  Free fatty acids promote hepatic lipotoxicity by stimulating TNF‐α expression via a lysosomal pathway , 2004 .

[20]  A. Volchuk,et al.  Chronic palmitate but not oleate exposure induces endoplasmic reticulum stress, which may contribute to INS-1 pancreatic beta-cell apoptosis. , 2006, Endocrinology.

[21]  E. Kabagambe,et al.  Adipose tissue biomarkers of fatty acid intake. , 2002, The American journal of clinical nutrition.

[22]  G. Gores,et al.  Kupffer cell engulfment of apoptotic bodies stimulates death ligand and cytokine expression , 2003, Hepatology.

[23]  W. Dowhan,et al.  Decreased cardiolipin synthesis corresponds with cytochrome c release in palmitate-induced cardiomyocyte apoptosis. , 2001, The Journal of biological chemistry.

[24]  D. Green Death and NF-kappaB in T cell activation: life at the edge. , 2003, Molecular cell.

[25]  Dong Wang,et al.  Saturated fatty acids induce endoplasmic reticulum stress and apoptosis independently of ceramide in liver cells. , 2006, American journal of physiology. Endocrinology and metabolism.

[26]  S. McCall,et al.  Inhibiting triglyceride synthesis improves hepatic steatosis but exacerbates liver damage and fibrosis in obese mice with nonalcoholic steatohepatitis , 2007, Hepatology.

[27]  Z. Younossi,et al.  Nonalcoholic fatty liver disease: a spectrum of clinical and pathological severity. , 1999, Gastroenterology.

[28]  G. Gores,et al.  Apoptotic Body Engulfment by a Human Stellate Cell Line Is Profibrogenic , 2003, Laboratory Investigation.

[29]  Shinobu Tanaka,et al.  Enhanced GLUT2 gene expression in an oleic acid-induced in vitro fatty liver model. , 2002, Hepatology research : the official journal of the Japan Society of Hepatology.

[30]  H. Cortez‐Pinto,et al.  Hepatocyte Apoptosis, Expression of Death Receptors, and Activation of NF-κB in the Liver of Nonalcoholic and Alcoholic Steatohepatitis Patients , 2004, The American Journal of Gastroenterology.

[31]  J. Maher,et al.  Anti-Fas Induces Hepatic Chemokines and Promotes Inflammation by an NF-κB-independent, Caspase-3-dependent Pathway* , 2001, The Journal of Biological Chemistry.

[32]  S. Banni,et al.  Characterization of conjugated diene fatty acids in milk, dairy products, and lamb tissues , 1996 .

[33]  M. Birnbaum,et al.  Role of Akt/protein kinase B in metabolism , 2002, Trends in Endocrinology & Metabolism.

[34]  G. Gores,et al.  Free fatty acids sensitise hepatocytes to TRAIL mediated cytotoxicity , 2007, Gut.

[35]  G. Gores,et al.  Steatohepatitis and Apoptosis: Therapeutic Implications , 2004, The American Journal of Gastroenterology.

[36]  Robert V Farese,et al.  Triglyceride accumulation protects against fatty acid-induced lipotoxicity , 2003, Proceedings of the National Academy of Sciences of the United States of America.

[37]  A. Cárabez,et al.  Expression of some hepatocyte-like functional properties of WRL-68 cells in culture , 1994, In Vitro Cellular & Developmental Biology - Animal.

[38]  D. Green Death and NF-κB in T Cell Activation: Life at the Edge , 2003 .

[39]  R. Rodrigo,et al.  Oxidative stress and depletion of hepatic long-chain polyunsaturated fatty acids may contribute to nonalcoholic fatty liver disease. , 2004, Free radical biology & medicine.

[40]  T. Yamane,et al.  Phenotypical stability of a human hepatoma cell line, HuH-7, in long-term culture with chemically defined medium. , 1984, Gan.

[41]  G. Wolfbauer,et al.  Secretion of lipids, apolipoproteins, and lipoproteins by human hepatoma cell line, HepG2: effects of oleic acid and insulin. , 1987, Journal of lipid research.

[42]  E. Siles,et al.  Steatosis recovery after treatment with a balanced sunflower or olive oil-based diet: involvement of perisinusoidal stellate cells. , 2005, World journal of gastroenterology.

[43]  C. Schmitz‐Peiffer,et al.  Ceramide Generation Is Sufficient to Account for the Inhibition of the Insulin-stimulated PKB Pathway in C2C12 Skeletal Muscle Cells Pretreated with Palmitate* , 1999, The Journal of Biological Chemistry.

[44]  M. Ohrvall,et al.  Fatty acid composition and estimated desaturase activities are associated with obesity and lifestyle variables in men and women. , 2006, Nutrition, metabolism, and cardiovascular diseases : NMCD.

[45]  J. McGarry Banting lecture 2001: dysregulation of fatty acid metabolism in the etiology of type 2 diabetes. , 2002, Diabetes.

[46]  G. Gores,et al.  Free Fatty Acids Induce JNK-dependent Hepatocyte Lipoapoptosis* , 2006, Journal of Biological Chemistry.

[47]  Keith D. Lindor,et al.  Nutritional and Metabolic Considerations in the Etiology of Nonalcoholic Steatohepatitis , 2001, Digestive Diseases and Sciences.

[48]  S. Clarke The multi-dimensional regulation of gene expression by fatty acids: polyunsaturated fats as nutrient sensors , 2004, Current opinion in lipidology.

[49]  G. Gores,et al.  Cathepsin B inactivation attenuates hepatic injury and fibrosis during cholestasis. , 2003, The Journal of clinical investigation.

[50]  G. Musso,et al.  Dietary habits and their relations to insulin resistance and postprandial lipemia in nonalcoholic steatohepatitis , 2003, Hepatology.

[51]  D. Bauman,et al.  Conjugated linoleic acid-enriched butter fat alters mammary gland morphogenesis and reduces cancer risk in rats. , 1999, The Journal of nutrition.

[52]  S. Sanderson,et al.  The natural history of nonalcoholic fatty liver disease: a population-based cohort study. , 2005, Gastroenterology.

[53]  Michelle M Wiest,et al.  A lipidomic analysis of nonalcoholic fatty liver disease , 2007, Hepatology.

[54]  R. Evans,et al.  Nuclear receptors and lipid physiology: opening the X-files. , 2001, Science.

[55]  B. Neuschwander‐Tetri,et al.  Nonalcoholic Fatty Liver Disease and Nonalcoholic Steatohepatitis , 2020, Clinical Gastroenterology and Hepatology.

[56]  M J May,et al.  NF-kappa B and Rel proteins: evolutionarily conserved mediators of immune responses. , 1998, Annual review of immunology.

[57]  M. A. Croce,et al.  17β‐estradiol prevents cytotoxicity from hydrophobic bile acids in HepG2 and WRL‐68 cell cultures , 2006, Journal of gastroenterology and hepatology.

[58]  H. Cortez‐Pinto,et al.  How different is the dietary pattern in non-alcoholic steatohepatitis patients? , 2006, Clinical nutrition.

[59]  L. Orci,et al.  Lipoapoptosis: its mechanism and its diseases. , 2002, Biochimica et biophysica acta.

[60]  J. Folch,et al.  A simple method for the isolation and purification of total lipides from animal tissues. , 1957, The Journal of biological chemistry.

[61]  G. Gores,et al.  Diet associated hepatic steatosis sensitizes to Fas mediated liver injury in mice. , 2003, Journal of hepatology.

[62]  Daniel S. Ory,et al.  Palmitate-induced Apoptosis Can Occur through a Ceramide-independent Pathway* , 2001, The Journal of Biological Chemistry.