Myoplasmic [Ca2+] Determines Myosin Phosphorylation and Isometric Stress in Agonist‐Stimulated Swine Arterial Smooth Muscle

Smooth muscle cells can regulate both their rate of stress development and the level of maintained stress. Agonist-induced steady-state stress was dependent on changes in aequorin-estimated myoplasmic [Ca2+] in the range of 120–190 nM. Higher levels of [Ca2+] were observed only transiently after stimulation and correlated with higher levels of myosin phosphorylation and faster stress development. A single regulatory system (Ca2+-dependent myosin light-chain phosphorylation) appears to control both mean crossbridge cycling rates (rate of contraction or shortening velocity) and the number of attached crossbridges (stress).