HISTOCHEMISTRY OF TISSUE CULTURE CELLS: A STUDY OF THE EFFECTS OF FREEZING AND OF SOME FIXATIVES

It has been shown that a) the membranes of HeLa and L cell lysosomes are readily permeable to naphthol AS-AM phosphate when used as a substrate for acid phosphatase, b) freezing as well as fixation affect the permeability of the lysosomal membrane to both naphthol AS-AM phosphate and β-glycerophosphate, c) none of these treatments seem to affect greatly the plasma or the mitochondrial membranes and d) the distribution of phospholipids in the cells is also affected by freezing or fixation.

[1]  N. Gonatas,et al.  DYNAMICS OF ACRIDINE ORANGE-CELL INTERACTION , 1964, The Journal of cell biology.

[2]  N. Gonatas,et al.  DYNAMICS OF ACRIDINE ORANGE-CELL INTERACTION. II. DYE-INDUCED ULTRASTRUCTURAL CHANGES IN MULTIVESICULAR BODIES (ACRIDINE ORANGE PARTICLES). , 1964 .

[3]  E. Robbins,et al.  DYNAMICS OF ACRIDINE ORANGE-CELL INTERACTION. I. INTERRELATIONSHIPS OF ACRIDINE ORANGE PARTICLES AND CYTOPLASMIC REDDENING. , 1963 .

[4]  G. J. Cunningham,et al.  The preservation of cytological and histochemical detail by a controlled temperature freezing and sectioning technique. , 1961, Annales d'histochimie.

[5]  N. Mizuno,et al.  LYSOSOMES IN CULTURED CELLS , 1961, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[6]  S. J. Holt,et al.  Preservation of Integrity of Rat Tissues for Cytochemical Staining Purposes , 1960, The Journal of biophysical and biochemical cytology.

[7]  C. de Duve,et al.  Tissue fractionation studies. 9. Enzymic release of bound hydrolases. , 1959, The Biochemical journal.

[8]  M. S. Burstone.  Histochemical demonstration of acid phosphatases with naphthol AS-phosphates. , 1958, Journal of the National Cancer Institute.

[9]  R. Wattiaux,et al.  Tissue fractionation studies. 6. Intracellular distribution patterns of enzymes in rat-liver tissue. , 1955, The Biochemical journal.

[10]  C. de Duve,et al.  Tissue fractionation studies. 3. Further observations on the binding of acid phosphatase by rat-liver particles. , 1955, The Biochemical journal.

[11]  R. Wattiaux,et al.  Tissue fractionation studies. 5. The association of acid phosphatase with a special class of cytoplasmic granules in rat liver. , 1955, The Biochemical journal.

[12]  M. Schwartz,et al.  Acid phosphatases; a review. , 1954, American journal of clinical pathology.

[13]  O. H. Lowry,et al.  THE QUANTITATIVE HISTOCHEMISTRY OF THE BRAIN , 1953, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[14]  J. Baker.  The histochemical recognition of lipine. , 1946, The Quarterly journal of microscopical science.

[15]  G. Gomori.  Microtechnical Demonstration of Phosphatase in Tissue Sections.∗ † , 1939 .

[16]  M. S. Burstone.  Enzyme Histochemistry, and Its Application in the Study of Neoplasms , 1962 .

[17]  M. Wolman.  Problems of Fixation in Cytology, Histology, and Histochemistry , 1955 .

[18]  G. Gomori.  An Improved Histochemical Technic for Acid Phosphatase , 1950 .