Expanding the metabolic engineering toolbox: more options to engineer cells.

[1]  J. Bailey,et al.  Influence of dilution rate and induction of cloned gene expression in continuous fermentations of recombinant yeast , 1991, Biotechnology and bioengineering.

[2]  Stephanopoulos,et al.  Metabolite and isotopomer balancing in the analysis of metabolic cycles: I. Theory. , 1999, Biotechnology and bioengineering.

[3]  A J Sinskey,et al.  Metabolite and isotopomer balancing in the analysis of metabolic cycles: II. Applications. , 1999, Biotechnology and bioengineering.

[4]  G. Stephanopoulos.  Metabolic fluxes and metabolic engineering. , 1999, Metabolic engineering.

[5]  W. Wiechert.  13C metabolic flux analysis. , 2001, Metabolic engineering.

[6]  J. Vervoort,et al.  19F NMR metabolomics for the elucidation of microbial degradation pathways of fluorophenols , 2001, Journal of Industrial Microbiology and Biotechnology.

[7]  Jens Nielsen,et al.  Analysis of flux estimates based on (13)C-labelling experiments. , 2002, European journal of biochemistry.

[8]  B. Palsson,et al.  Escherichia coli K-12 undergoes adaptive evolution to achieve in silico predicted optimal growth , 2002, Nature.

[9]  Vassily Hatzimanikatis,et al.  Inverse metabolic engineering: a strategy for directed genetic engineering of useful phenotypes. , 2002, Biotechnology and bioengineering.

[10]  Christopher A. Voigt,et al.  Protein building blocks preserved by recombination , 2002, Nature Structural Biology.

[11]  P. Shannon,et al.  Cytoscape: a software environment for integrated models of biomolecular interaction networks. , 2003, Genome research.

[12]  J. Keasling,et al.  Engineering a mevalonate pathway in Escherichia coli for production of terpenoids , 2003, Nature Biotechnology.

[13]  A. Burgard,et al.  Optknock: A bilevel programming framework for identifying gene knockout strategies for microbial strain optimization , 2003, Biotechnology and bioengineering.

[14]  M. Tomita,et al.  Quantitative metabolome analysis using capillary electrophoresis mass spectrometry. , 2003, Journal of proteome research.

[15]  B. Palsson,et al.  Saccharomyces cerevisiae phenotypes can be predicted by using constraint-based analysis of a genome-scale reconstructed metabolic network , 2003, Proceedings of the National Academy of Sciences of the United States of America.

[16]  Byung-Kwon Choi,et al.  Use of combinatorial genetic libraries to humanize N-linked glycosylation in the yeast Pichia pastoris , 2003, Proceedings of the National Academy of Sciences of the United States of America.

[17]  C. Nakamura,et al.  Metabolic engineering for the microbial production of 1,3-propanediol. , 2003, Current opinion in biotechnology.

[18]  David W. Colby,et al.  Potent inhibition of huntingtin aggregation and cytotoxicity by a disulfide bond-free single-domain intracellular antibody. , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[19]  G. Church,et al.  Accurate multiplex gene synthesis from programmable DNA microchips , 2004, Nature.

[20]  Martin Fussenegger,et al.  Gas-inducible transgene expression in mammalian cells and mice , 2004, Nature Biotechnology.

[21]  G. Stephanopoulos,et al.  Exploiting biological complexity for strain improvement through systems biology , 2004, Nature Biotechnology.

[22]  Sang Yup Lee,et al.  The genome sequence of the capnophilic rumen bacterium Mannheimia succiniciproducens , 2004, Nature Biotechnology.

[23]  Roded Sharan,et al.  PathBLAST: a tool for alignment of protein interaction networks , 2004, Nucleic Acids Res..

[24]  Meng H. Heng,et al.  Characterization of Humanized Antibodies Secreted by Aspergillus niger , 2004, Applied and Environmental Microbiology.

[25]  T. W. Jeffries,et al.  Metabolic engineering for improved fermentation of pentoses by yeasts , 2004, Applied Microbiology and Biotechnology.

[26]  J. Venter,et al.  The Century of Biology , 2004 .

[27]  Mohamed Al-Rubeai,et al.  Uncoupling of cell growth and proliferation results in enhancement of productivity in p21CIP1‐arrested CHO cells , 2004, Biotechnology and bioengineering.

[28]  M. Fussenegger,et al.  An engineered epigenetic transgene switch in mammalian cells , 2004, Nature Biotechnology.

[29]  J. Pronk,et al.  Minimal metabolic engineering of Saccharomyces cerevisiae for efficient anaerobic xylose fermentation: a proof of principle. , 2004, FEMS yeast research.

[30]  F. Arnold,et al.  Laboratory evolution of cytochrome P450 BM‐3 monooxygenase for organic cosolvents , 2004, Biotechnology and bioengineering.

[31]  Martin Fussenegger,et al.  Hysteresis in a synthetic mammalian gene network. , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[32]  Thomas A Kost,et al.  Baculovirus as versatile vectors for protein expression in insect and mammalian cells , 2005, Nature Biotechnology.

[33]  G. Stephanopoulos,et al.  Tuning genetic control through promoter engineering. , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[34]  Bernhard O. Palsson,et al.  Network-level analysis of metabolic regulation in the human red blood cell using random sampling and singular value decomposition , 2006, BMC Bioinformatics.

[35]  M. Mann,et al.  Mass spectrometry–based proteomics turns quantitative , 2005, Nature chemical biology.

[36]  Marc Ostermeier,et al.  Directed evolution of protein switches and their application to the creation of ligand-binding proteins. , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[37]  G. Lee,et al.  Influence of down-regulation of caspase-3 by siRNAs on sodium-butyrate-induced apoptotic cell death of Chinese hamster ovary cells producing thrombopoietin. , 2005, Metabolic engineering.

[38]  Timo K. Korhonen,et al.  Extracellular secretion of polypeptides using a modified Escherichia coli flagellar secretion apparatus , 2005, Nature Biotechnology.

[39]  Gregory Stephanopoulos,et al.  Construction of lycopene-overproducing E. coli strains by combining systematic and combinatorial gene knockout targets , 2005, Nature Biotechnology.

[40]  Gregory Stephanopoulos,et al.  High-throughput metabolic state analysis: the missing link in integrated functional genomics of yeasts. , 2005, The Biochemical journal.

[41]  J. Liao,et al.  A synthetic gene–metabolic oscillator , 2005, Nature.

[42]  U. Sauer,et al.  Large-scale in vivo flux analysis shows rigidity and suboptimal performance of Bacillus subtilis metabolism , 2005, Nature Genetics.

[43]  G. Stephanopoulos,et al.  Identifying gene targets for the metabolic engineering of lycopene biosynthesis in Escherichia coli. , 2005, Metabolic engineering.

[44]  G. Chiang,et al.  Bcl-xL mediates increased production of humanized monoclonal antibodies in Chinese hamster ovary cells , 2005 .

[45]  M. Koffas,et al.  Biosynthesis of Natural Flavanones in Saccharomyces cerevisiae , 2005, Applied and Environmental Microbiology.

[46]  Travis S. Bayer,et al.  Programmable ligand-controlled riboregulators of eukaryotic gene expression , 2005, Nature Biotechnology.

[47]  Keith E. J. Tyo,et al.  High-Throughput Screen for Poly-3-Hydroxybutyrate in Escherichia coli and Synechocystis sp. Strain PCC6803 , 2006, Applied and Environmental Microbiology.

[48]  Timothy S. Ham,et al.  Production of the antimalarial drug precursor artemisinic acid in engineered yeast , 2006, Nature.

[49]  Youwei Jiang,et al.  Optimization of humanized IgGs in glycoengineered Pichia pastoris , 2006, Nature Biotechnology.

[50]  G. Stephanopoulos,et al.  Engineering Yeast Transcription Machinery for Improved Ethanol Tolerance and Production , 2006, Science.

[51]  M. Koffas,et al.  Functional expression of a P450 flavonoid hydroxylase for the biosynthesis of plant-specific hydroxylated flavonols in Escherichia coli. , 2006, Metabolic engineering.

[52]  Thomas E. Ferrin,et al.  Designed divergent evolution of enzyme function , 2006, Nature.

[53]  Nicholas J. Guido,et al.  A bottom-up approach to gene regulation , 2006, Nature.

[54]  G. Stephanopoulos,et al.  Global transcription machinery engineering: a new approach for improving cellular phenotype. , 2007, Metabolic engineering.