Expression of genes associated with aroma formation derived from the fatty acid pathway during peach fruit ripening.

Changes in characteristic aroma volatiles, levels of fatty acids as aroma precursors, and expression patterns of related genes, including lipoxygenase (LOX), hydroperoxide lyase (HPL), alcohol dehydrogenase (ADH), alcohol acyltransferase (AAT), and fatty acid desaturase (FAD), were studied in peach ( Prunus persica L. Batsch., cv. Yulu) fruit during postharvest ripening at 20 degrees C. Concentrations of n-hexanal, (E)-2-hexenal, (E)-2-hexenol, and (Z)-3-hexenol decreased, whereas the production of (Z)-3-hexenyl acetate, gamma-hexalactone, gamma-octalactone, gamma-decalactone, and delta-decalactone increased with fruit ripening. Lactones showed a clear pattern concomitant with the climacteric rise in ethylene production, with gamma-decalactone being the principal volatile compound at the late ripening stage. Of the LOX family genes, PpLOX2 and PpLOX3 had relatively high transcript levels initially followed by a decline with fruit ripening, while levels of PpLOX1 and PpLOX4 transcripts were upregulated by accumulated ethylene production. Expression of PpHPL1, PpADH1, PpADH2, and PpADH3 showed similar decreasing patterns during ripening. Expression levels of PpAAT1 showed a rapid increase during the first 2 days of postharvest ripening followed by a gradual decrease. Contents of polyunsaturated linoleic and linolenic acids increased, and saturated palmitic acid levels tended to decline as the fruit ripened. The increased levels of unsaturated fatty acids closely paralleled increasing expression of PpFAD1 and PpFAD2. The significance of gene expression changes in relation to aroma volatile production is discussed.

[1]  G. Echeverría,et al.  Overall quality of 'Rich Lady' peach fruit after air- or CA storage. The importance of volatile emission , 2009 .

[2]  Shaohua Li,et al.  Volatile characteristics of 50 peaches and nectarines evaluated by HP-SPME with GC-MS , 2009 .

[3]  C. Sanz,et al.  Functional characterization of two 13-lipoxygenase genes from olive fruit in relation to the biosynthesis of volatile compounds of virgin olive oil. , 2009, Journal of agricultural and food chemistry.

[4]  Kun-song Chen,et al.  Volatiles Production and Lipoxygenase Gene Expression in Kiwifruit Peel and Flesh During Fruit Ripening , 2009 .

[5]  Reinaldo Campos-Vargas,et al.  Differential expression levels of aroma-related genes during ripening of apricot (Prunus armeniaca L.). , 2009, Plant physiology and biochemistry : PPB.

[6]  Kun-song Chen,et al.  Lipoxygenase gene expression in ripening kiwifruit in relation to ethylene and aroma production. , 2009, Journal of agricultural and food chemistry.

[7]  S. Kays,et al.  Effect of hyperbaric, controlled atmosphere, and UV treatments on peach volatiles , 2009 .

[8]  R. Infante,et al.  Quantitative determination of flesh mealiness in peach [Prunus persica L. (Batch.)] through paper absorption of free juice , 2009 .

[9]  Kietsuda Luengwilai,et al.  Chapter 1 Aroma Volatiles: Biosynthesis and Mechanisms of Modulation During Fruit Ripening , 2009 .

[10]  Zhen Zhang,et al.  Selection of reliable reference genes for gene expression studies in peach using real-time PCR , 2009, BMC Molecular Biology.

[11]  A. Vecchietti,et al.  Comparative analysis of expressed sequence tags from tissues in ripening stages of peach (Prunus persica L. Batsch) , 2009, Tree Genetics & Genomes.

[12]  Yanmin Zhu,et al.  Characterization of cultivar differences in alcohol acyltransferase and 1-aminocyclopropane-1-carboxylate synthase gene expression and volatile ester emission during apple fruit maturation and ripening , 2008 .

[13]  R. Infante,et al.  Monitoring the sensorial quality and aroma through an electronic nose in peaches during cold storage , 2008 .

[14]  C. Bonghi,et al.  Transcriptome profiling of ripening nectarine (Prunus persica L. Batsch) fruit treated with 1-MCP. , 2008, Journal of experimental botany.

[15]  W. Schwab,et al.  Biosynthesis of plant-derived flavor compounds. , 2008, The Plant journal : for cell and molecular biology.

[16]  M. R. Tabilio,et al.  Effects of cold storage on aroma compounds of white- and yellow-fleshed peaches , 2008 .

[17]  E. Pichersky,et al.  Metabolic engineering of plant volatiles. , 2008, Current opinion in biotechnology.

[18]  R. Welti,et al.  Tocopherols Modulate Extraplastidic Polyunsaturated Fatty Acid Metabolism in Arabidopsis at Low Temperature[W] , 2008, The Plant Cell Online.

[19]  G. Casadoro,et al.  The involvement of auxin in the ripening of climacteric fruits comes of age: the hormone plays a role of its own and has an intense interplay with ethylene in ripening peaches. , 2007, Journal of experimental botany.

[20]  Robert J. Schaffer,et al.  A Genomics Approach Reveals That Aroma Production in Apple Is Controlled by Ethylene Predominantly at the Final Step in Each Biosynthetic Pathway[W] , 2007, Plant Physiology.

[21]  T. Nishioka,et al.  Changing green leaf volatile biosynthesis in plants: An approach for improving plant resistance against both herbivores and pathogens , 2006, Proceedings of the National Academy of Sciences.

[22]  Kun-song Chen,et al.  Differential expression within the LOX gene family in ripening kiwifruit. , 2006, Journal of experimental botany.

[23]  J. Pech,et al.  Two highly divergent alcohol dehydrogenases of melon exhibit fruit ripening-specific expression and distinct biochemical characteristics , 2006, Plant Molecular Biology.

[24]  R. Beaudry,et al.  Deficiency of Linolenic Acid in Lefad7 Mutant Tomato Changes the Volatile Profile and Sensory Perception of Disrupted Leaf and Fruit Tissue , 2006 .

[25]  Luciano Milanesi,et al.  ESTree db: a Tool for Peach Functional Genomics , 2005, BMC Bioinformatics.

[26]  J. Pech,et al.  Functional Characterization of a Melon Alcohol Acyl-transferase Gene Family Involved in the Biosynthesis of Ester Volatiles. Identification of the Crucial Role of a Threonine Residue for Enzyme Activity* , 2005, Plant Molecular Biology.

[27]  F. Mencarelli,et al.  Inhibition of ethylene via different ways affects LOX and ADH activities, and related volatiles compounds in peach (cv. 'Royal Gem') , 2005 .

[28]  A. Dandekar,et al.  Apple aroma : alcohol acyltransferase, a rate limiting step for ester biosynthesis, is regulated by ethylene , 2005 .

[29]  D. L. García-González,et al.  Impact of the suppression of lipoxygenase and hydroperoxide lyase on the quality of the green odor in green leaves. , 2005, Journal of agricultural and food chemistry.

[30]  D. Grierson,et al.  Identification of a Specific Isoform of Tomato Lipoxygenase (TomloxC) Involved in the Generation of Fatty Acid-Derived Flavor Compounds1 , 2004, Plant Physiology.

[31]  C. Brady,et al.  Structure of the tomato Adh2 gene and Adh2 pseudogenes, and a study of Adh2 gene expression in fruit , 1994, Plant Molecular Biology.

[32]  Inmaculada Recasens,et al.  Multivariate analysis of maturity stages, including quality and aroma in `Royal Glory' peaches and `Big Top' nectarines , 2002 .

[33]  G. Griffiths,et al.  Lipoxygenase H1 Gene Silencing Reveals a Specific Role in Supplying Fatty Acid Hydroperoxides for Aliphatic Aldehyde Production* , 2002, The Journal of Biological Chemistry.

[34]  K. Matsui,et al.  Effect of overexpression of fatty acid 9-hydroperoxide lyase in tomatoes (Lycopersicon esculentum Mill.). , 2001, Journal of agricultural and food chemistry.

[35]  C. Ho,et al.  Modification of fatty acids changes the flavor volatiles in tomato leaves. , 2001, Phytochemistry.

[36]  J. Sánchez-Serrano,et al.  Hydroperoxide lyase depletion in transgenic potato plants leads to an increase in aphid performance , 2001, Proceedings of the National Academy of Sciences of the United States of America.

[37]  A. Brash,et al.  Biogenesis of volatile aldehydes from fatty acid hydroperoxides: molecular cloning of a hydroperoxide lyase (CYP74C) with specificity for both the 9- and 13-hydroperoxides of linoleic and linolenic acids. , 2001, Archives of biochemistry and biophysics.

[38]  A. Brash,et al.  Purification, molecular cloning, and expression of the gene encoding fatty acid 13-hydroperoxide lyase from guava fruit (Psidium guajava) , 2000, Lipids.

[39]  G. Howe,et al.  Cytochrome P450-dependent metabolism of oxylipins in tomato. Cloning and expression of allene oxide synthase and fatty acid hydroperoxide lyase. , 2000, Plant physiology.

[40]  Hilko van der Voet,et al.  Identification of the SAAT Gene Involved in Strawberry Flavor Biogenesis by Use of DNA Microarrays , 2000, Plant Cell.

[41]  D. Grierson,et al.  Ethylene and developmental signals regulate expression of lipoxygenase genes during tomato fruit ripening , 1999 .

[42]  Lee,et al.  Genetic manipulation of alcohol dehydrogenase levels in ripening tomato fruit affects the balance of some flavor aldehydes and alcohols , 1998, Plant physiology.

[43]  M. Vanoli,et al.  Volatile compound production during growth and ripening of peaches and nectarines , 1997 .

[44]  Chi-Tang Ho,et al.  Changes of Fatty Acids and Fatty Acid-Derived Flavor Compounds by Expressing the Yeast Δ-9 Desaturase Gene in Tomato , 1996 .

[45]  A. Scartazza,et al.  Lipid evolution during development and ripening of peach fruits , 1995 .

[46]  W. R. Forbus,et al.  Physical and chemical changes during the maturation of peaches (cv. Majestic). , 1991 .

[47]  A. Ohmiya,et al.  Changes in the Composition and Content of Volatile Constituents in Peach Fruits in Relation to Maturity at Harvest and Artificial Ripening , 1991 .

[48]  Robert J. Horvat,et al.  Comparison of volatile compounds from peach fruit and leaves (cv. Monroe) during maturation. , 1990 .

[49]  J. Robertson,et al.  Effect of cold storage and maturity on the physical and chemical characteristics and volatile constituents of peaches (cv. Cresthaven) , 1990 .

[50]  James A. Robertson,et al.  Comparison of the volatile compounds from several commercial peach cultivars , 1990 .