Long-term stem cell labeling by collagen-functionalized single-walled carbon nanotubes.

The monitoring of grafted stem cells is crucial to assess the efficiency, effectiveness and safety of such stem cell-based therapies. In this regard, a reliable and cytocompatible labeling method for stem cells is critically needed. In this study, the collagen-functionalized single-walled carbon nanotubes (Col-SWCNTs) were used as imaging probes for labeling of human mesenchymal stem cells (hMSCs) and the inherent Raman scattering of SWCNTs was used to image the SWCNT-labeled cells. The results showed that the Col-SWCNTs exhibit efficient cellular internalization by hMSCs without affecting their proliferation and differentiation. The prolonged dwell time of Col-SWCNTs in cells ensured the long-term labeling for up to 2 weeks. This work reveals the potential of Col-SWCNTs as probes for long-term stem cell labeling.

[1]  N. Kawazoe,et al.  Uptake and intracellular distribution of collagen-functionalized single-walled carbon nanotubes. , 2013, Biomaterials.

[2]  Omar K. Yaghi,et al.  Ultra-low doses of chirality sorted (6,5) carbon nanotubes for simultaneous tumor imaging and photothermal therapy. , 2013, ACS nano.

[3]  C. Wilhelm,et al.  Endowing carbon nanotubes with superparamagnetic properties: applications for cell labeling, MRI cell tracking and magnetic manipulations. , 2013, Nanoscale.

[4]  Xiaoke Zhang,et al.  Single walled carbon nanotubes as drug delivery vehicles: targeting doxorubicin to tumors. , 2012, Biomaterials.

[5]  M. Prato,et al.  Cellular uptake mechanisms of functionalised multi-walled carbon nanotubes by 3D electron tomography imaging. , 2011, Nanoscale.

[6]  K Kostarelos,et al.  Promises, facts and challenges for carbon nanotubes in imaging and therapeutics. , 2009, Nature nanotechnology.

[7]  H. Dai,et al.  Preparation of carbon nanotube bioconjugates for biomedical applications , 2009, Nature Protocols.

[8]  Decai Yu,et al.  Cell response to carbon nanotubes: size-dependent intracellular uptake mechanism and subcellular fate. , 2010, Small.

[9]  K. Neoh,et al.  Surface modification of magnetic nanoparticles for stem cell labeling , 2012 .

[10]  I. Weissman,et al.  Stem cells, cancer, and cancer stem cells , 2001, Nature.

[11]  Jochen Ringe,et al.  Highly efficient magnetic stem cell labeling with citrate-coated superparamagnetic iron oxide nanoparticles for MRI tracking. , 2012, Biomaterials.

[12]  Rafael Yuste,et al.  Fluorescence microscopy today , 2005, Nature Methods.

[13]  J. Dai,et al.  Magnetic resonance imaging of Fe3O4@SiO2-labeled human mesenchymal stem cells in mice at 11.7 T. , 2013, Biomaterials.

[14]  T. Fujigaya,et al.  Enhanced cell uptake via non-covalent decollation of a single-walled carbon nanotube-DNA hybrid with polyethylene glycol-grafted poly(l-lysine) labeled with an Alexa-dye and its efficient uptake in a cancer cell. , 2011, Nanoscale.

[15]  R. Smalley,et al.  Structure-Assigned Optical Spectra of Single-Walled Carbon Nanotubes , 2002, Science.

[16]  K. Kraus,et al.  Fluorescently labeled mesenchymal stem cells (MSCs) maintain multilineage potential and can be detected following implantation into articular cartilage defects. , 2002, Biomaterials.

[17]  M. Kay,et al.  Sarcoma Derived from Cultured Mesenchymal Stem Cells , 2007, Stem cells.

[18]  Zhenzhong Zhang,et al.  Synergistic anticancer effect of RNAi and photothermal therapy mediated by functionalized single-walled carbon nanotubes. , 2013, Biomaterials.

[19]  Daniel A. De Ugarte,et al.  In Vivo Distribution of Human Adipose‐Derived Mesenchymal Stem Cells in Novel Xenotransplantation Models , 2007, Stem cells.

[20]  Bing Yan,et al.  Endosomal leakage and nuclear translocation of multiwalled carbon nanotubes: developing a model for cell uptake. , 2009, Nano letters.

[21]  Xiaoyuan Chen,et al.  Nanoparticles for cell labeling. , 2011, Nanoscale.

[22]  M. Prato,et al.  Cellular uptake of functionalized carbon nanotubes is independent of functional group and cell type. , 2007, Nature nanotechnology.

[23]  Kenneth A. Dawson,et al.  Role of cell cycle on the cellular uptake and dilution of nanoparticles in a cell population. , 2011, Nature nanotechnology.

[24]  K. Dahl,et al.  Quantification of uptake and localization of bovine serum albumin-stabilized single-wall carbon nanotubes in different human cell types. , 2011, Small.

[25]  Mark C. Hersam,et al.  Band Gap Photobleaching in Isolated Single-Walled Carbon Nanotubes , 2003 .

[26]  Shih-Chang Wang,et al.  Biodegradable magnetic-fluorescent magnetite/poly(dl-lactic acid-co-alpha,beta-malic acid) composite nanoparticles for stem cell labeling. , 2010, Biomaterials.

[27]  Ronghua Yang,et al.  Single-walled carbon nanotubes as optical materials for biosensing. , 2011, Nanoscale.

[28]  M. Pittenger,et al.  Multilineage potential of adult human mesenchymal stem cells. , 1999, Science.

[29]  J. McFadden,et al.  Uptake and Release of Double‐Walled Carbon Nanotubes by Mammalian Cells , 2010 .

[30]  J. Hubbell,et al.  Biocompatible dispersions of carbon nanotubes: a potential tool for intracellular transport of anticancer drugs. , 2011, Nanoscale.

[31]  M. Prato,et al.  Intracellular Trafficking of Carbon Nanotubes by Confocal Laser Scanning Microscopy , 2007 .

[32]  P. Midgley,et al.  Uptake of noncytotoxic acid-treated single-walled carbon nanotubes into the cytoplasm of human macrophage cells. , 2009, ACS nano.

[33]  Kai Yang,et al.  Carbon materials for drug delivery & cancer therapy , 2011 .

[34]  N. Kaji,et al.  Quantum dots labeling using octa-arginine peptides for imaging of adipose tissue-derived stem cells. , 2010, Biomaterials.

[35]  I. Weissman,et al.  Translating stem and progenitor cell biology to the clinic: barriers and opportunities. , 2000, Science.