In vivo anti-Trichophyton Activities of Seed Oil Obtained from Caraganakorshinskii Kom.

Aim: The objective of this study was to evaluate the effects of seed oil of Caragana korshinskii Kom. against Trichophyton mentagrophytes on an in vivo guinea pig model of dermatophytosis. Methods: The skin of albino guinea pigs was infected with T. mentagrophytes, and the animals were divided into five groups: negative control (NC group), positive control (PC group), vehicle control, CK50% group (received topical 50% seed oil of C.korshinskii), and CK100% group (received topical 100% seed oil of C.korshinskii). Evaluation of clinical efficacy was performed 72 h after the completion of a 10-day treatment regimen. Skin biopsy samples were processed for histopathological examination. Results: The infected untreated control guinea pigs showed patches of hair loss and ulcerated or scaly skin. Lower clinical scores indicate improved efficacy compared with NC. The lesion scores significantly declined in the CK50%, CK100%, and PC groups in comparison with the NC group. The CK50% group (45.31%) and the CK100% group (75%) showed clinical efficacy compared with the PC group (78.13%). In addition, no fungal elements, inflammation, or tissue destruction was observed in any of the PAS-stained sections of the infected skin in the groups treated with CK100% or 1% terbinafine. Conclusion: Seed oil of C.korshinskii demonstrated high antifungal efficacy in experimental dermatophytosis.

[1]  A. Mikaeili,et al.  Antifungal activities of Astragalus verus Olivier. against Trichophyton verrucosum on in vitro and in vivo guinea pig model of dermatophytosis , 2012, Mycoses.

[2]  Xiaotao Hu,et al.  Leaf photosynthesis, chlorophyll fluorescence, ion content and free amino acids in Caragana korshinskii Kom exposed to NaCl stress , 2012, Acta Physiologiae Plantarum.

[3]  Suo You-rui.  Anti-fungal Activity of Seed Oil from Caragana korshinskii Kom. and Mechanism , 2012 .

[4]  M. S. Khan,et al.  Antifungal activity of essential oils and their synergy with fluconazole against drug-resistant strains of Aspergillus fumigatus and Trichophyton rubrum , 2011, Applied Microbiology and Biotechnology.

[5]  Reza Tolouei,et al.  Search for novel antifungals from 49 indigenous medicinal plants: Foeniculum vulgare and Platycladus orientalis as strong inhibitors of aflatoxin production by Aspergillus parasiticus , 2011, Annals of Microbiology.

[6]  Guobin Liu,et al.  Effects of Caragana Korshinskii Kom. cover on runoff, sediment yield and nitrogen loss , 2010 .

[7]  Reza Saberi,et al.  Effect of Matricaria chamomilla L. flower essential oil on the growth and ultrastructure of Aspergillus niger van Tieghem. , 2010, International journal of food microbiology.

[8]  U. Yusuf,et al.  In vitro antifungal activity of allicin alone and in combination with two medications against Trichophyton rubrum , 2010 .

[9]  J. Hanrahan,et al.  Ethnobotany, phytochemistry and pharmacology of the genus Caragana used in traditional Chinese medicine. , 2009, Journal of ethnopharmacology.

[10]  Y. Wu,et al.  Growth, water relations, and stomatal development of Caragana korshinskii Kom. and Zygophyllum xanthoxylum (Bunge) Maxim. seedlings in response to water deficits , 2009 .

[11]  Yuanrun Zheng,et al.  Effects of watering regime and depth of burial on seedling emergence of four dominant psammophytes in the Mu Us sandy land, Inner Mongolia, China, and relevance to revegetation of a desertified region , 2009 .

[12]  M. Ghannoum,et al.  Determination of the efficacy of terbinafine hydrochloride nail solution in the topical treatment of dermatophytosis in a guinea pig model , 2009, Mycoses.

[13]  Jun Yu,et al.  Antifungal activity of Pterocarpans from Caragana jubata (pall.) Poir. , 2009 .

[14]  M. Friedrich,et al.  Epidemiological trends in skin mycoses worldwide , 2008, Mycoses.

[15]  F Bakkali,et al.  Biological effects of essential oils--a review. , 2008, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[16]  M. Friedrich,et al.  Title:Epidemiological trends in skin mycoses worldwide. , 2008 .

[17]  Fengmin Li,et al.  Responses of Caragana korshinskii Kom. to shoot removal: mechanisms underlying regrowth , 2008, Ecological Research.

[18]  M. Ghannoum,et al.  Quality Control and Reference Guidelines for CLSI Broth Microdilution Method (M38-A Document) for Susceptibility Testing of Anidulafungin against Molds , 2007, Journal of Clinical Microbiology.

[19]  Daniel de Assis Santos,et al.  Evaluation of susceptibility of Trichophyton mentagrophytes and Trichophyton rubrum clinical isolates to antifungal drugs using a modified CLSI microdilution method (M38-A). , 2007, Journal of medical microbiology.

[20]  K. Rahman,et al.  Antinociceptive activity and chemical composition of constituents from Caragana microphylla seeds. , 2007, Phytomedicine : international journal of phytotherapy and phytopharmacology.

[21]  M. Shams-Ghahfarokhi,et al.  In vitro antifungal activities of Allium cepa, Allium sativum and ketoconazole against some pathogenic yeasts and dermatophytes. , 2006, Fitoterapia.

[22]  R. Kappe,et al.  In vitro susceptibility of 15 strains of zygomycetes to nine antifungal agents as determined by the NCCLS M38‐A microdilution method , 2005, Mycoses.

[23]  S. Çankaya,et al.  Comparison of in vitro activities of antifungal drugs and ethanolic extract of propolis against Trichophyton rubrum and T. mentagrophytes by using a microdilution assay , 2005, Mycoses.

[24]  A. Espinel-Ingroff,et al.  Comparison of the Sensititre YeastOne Colorimetric Antifungal Panel with a Modified NCCLS M38-A Method To Determine the Activity of Voriconazole against Clinical Isolates of Aspergillus spp , 2004, Journal of Clinical Microbiology.

[25]  M. Ghannoum,et al.  Evaluation of Antifungal Efficacy in an Optimized Animal Model of Trichophyton mentagrophytes-Dermatophytosis , 2004, Journal of chemotherapy.

[26]  B. L. Hainer.  Dermatophyte infections. , 2003, American family physician.

[27]  J. Guarro,et al.  Dermatophytes in domestic animals. , 2000 .

[28]  C. Curtis.  Use and abuse of topical dermatological therapy in dogs and cats Part 1. Shampoo therapy , 1998, In Practice.

[29]  T. Platts-Mills,et al.  The immune response to dermatophytes. , 1998, Research in immunology.

[30]  R. Summerbell,et al.  The dermatophytes , 1995, Clinical microbiology reviews.

[31]  D. Kelly,et al.  Mode of Action and Resistance to Azole Antifungals Associated with the Formation of 14α-Methylergosta-8,24(28)-dien-3β,6α-diol , 1995 .

[32]  H. Ishizaki,et al.  Decreased release of interferon-gamma by peripheral blood mononuclear cells of patients with chronic dermatophytosis in response to stimulation with trichophytin. , 1995, Acta dermato-venereologica.

[33]  Lindsey Jd,et al.  [Anti-inflammatory effect of Caragana microphylla Lam]. , 1993 .

[34]  R. Hay,et al.  Fungicidal activity of human neutrophils and monocytes on dermatophyte fungi, Trichophyton quinckeanum and Trichophyton rubrum. , 1987, Immunology.

[35]  L. W. Parks,et al.  Multiple functions for sterols in Saccharomyces cerevisiae. , 1985, Biochimica et biophysica acta.

[36]  R. Hay,et al.  Experimental dermatophytosis: the clinical and histopathologic features of a mouse model using Trichophyton quinckeanum (mouse favus). , 1983, The Journal of investigative dermatology.

[37]  F. Rastinejad,et al.  Fungistatic mechanism of human transferrin for Rhizopus oryzae and Trichophyton mentagrophytes: alternative to simple iron deprivation , 1983, Infection and immunity.

[38]  Khan Ha,et al.  Transferrin, iron, and dermatophytes. I. Serum dematophyte inhibitory component definitively identified as unsaturated transferrin. , 1975 .

[39]  R. D. King,et al.  Transferrin, iron, and dermatophytes. I. Serum dematophyte inhibitory component definitively identified as unsaturated transferrin. , 1975, The Journal of laboratory and clinical medicine.