Fiber-optic nanosensors for single-cell monitoring

This article is an overview of the fabrication, operating principles, and applications of fiber-optic nanobiosensors with the capability of in-vivo analysis at the single-cell level. Recently, the cross-disciplinary integration of nanotechnology, biology, and photonics has been revolutionizing important areas in molecular biology, especially diagnostics and therapy at the molecular and cellular level. Fiber-optic nanobiosensors are a unique class of biosensor that enable analytical measurements in individual living cells and the probing of individual chemical species in specific locations within a cell. This article provides a review of the research performed in our laboratory and discusses the usefulness and potential of this nanotechnology-based biosensor system in biological research and its applications to biomonitoring of individual cells.

[1]  R. Kopelman,et al.  Nitrite- and chloride-selective fluorescent nano-optodes and in vitro application to rat conceptuses. , 1998, Analytical chemistry.

[2]  G M Cohen,et al.  Caspases: the executioners of apoptosis. , 1997, The Biochemical journal.

[3]  Tuan Vo-Dinh,et al.  Antibody-Based Submicron Biosensor for Benzo[A]Pyrene DNA Adduct , 1996 .

[4]  T. Vo‐Dinh,et al.  Nanosensors for Single- Cell Analyses , 2003 .

[5]  Tuan Vo-Dinh,et al.  Evaluation of antibody immobilization technqiues for fiber optic-based fluoroimmunosensing , 1990 .

[6]  B M Cullum,et al.  The development of optical nanosensors for biological measurements. , 2000, Trends in biotechnology.

[7]  Joshua Samuel,et al.  Miniaturization of organically doped sol-gel materials: a microns-size fluorescent pH sensor , 1994 .

[8]  Robert J. Chichester,et al.  Single Molecules Observed by Near-Field Scanning Optical Microscopy , 1993, Science.

[9]  S. Mcculloch,et al.  Optical nanosensors — towards the development of intracellular monitoring , 1996 .

[10]  C. M. Braguglia.  Biosensors : An outline of general principles and application , 1998 .

[11]  E C Nice,et al.  Instrumental biosensors: new perspectives for the analysis of biomolecular interactions. , 1999, BioEssays : news and reviews in molecular, cellular and developmental biology.

[12]  Tuan Vo-Dinh,et al.  Detection of cytochrome C in a single cell using an optical nanobiosensor. , 2004, Analytical chemistry.

[13]  David R. Walt,et al.  Intramolecular fluorescence self-quenching of fluoresceinamine , 1990 .

[14]  T. Vo-Dinh,et al.  Antibody-Based Fiberoptics Biosensor for the Carcinogen Benzo(a)pyrene , 1987 .

[15]  R Zenobi,et al.  Near-Field Surface-Enhanced Raman Imaging of Dye-Labeled DNA with 100-nm Resolution. , 1998, Analytical chemistry.

[16]  Weihong Tan,et al.  Probing intracellular dynamics in living cells with near-field optics , 1999, Journal of Neuroscience Methods.

[17]  Tuan Vo-Dinh,et al.  Development of Nanosensors and Bioprobes , 2000 .

[18]  T. Vo‐Dinh,et al.  Nanosensor for in vivo measurement of the carcinogen benzo[a]pyrene in a single cell. , 2002, Journal of nanoscience and nanotechnology.

[19]  Michael T. Miller,et al.  Ultrasmall optical sensors for cellular measurements. , 1999, Analytical chemistry.

[20]  N. Thornberry,et al.  Caspases: killer proteases. , 1997, Trends in biochemical sciences.

[21]  J A Cox,et al.  Chemical and biochemical sensors based on advances in materials chemistry. , 1999, Journal of Pharmaceutical and Biomedical Analysis.

[22]  T. Vo‐Dinh,et al.  Optical sensor for the detection of caspase-9 activity in a single cell. , 2004, Journal of the American Chemical Society.

[23]  Tuan Vo-Dinh,et al.  NEAR-FIELD SURFACE-ENHANCED RAMAN SPECTROSCOPY OF DYE MOLECULES ADSORBED ON SILVER ISLAND FILMS , 1998 .

[24]  T. Vo‐Dinh,et al.  Intracellular measurements in mammary carcinoma cells using fiber-optic nanosensors. , 2000, Analytical biochemistry.

[25]  Raoul Kopelman,et al.  Development of submicron chemical fiber optic sensors , 1992 .

[26]  Dinh Tuan Vo.  Chemical analysis of polycyclic aromatic compounds , 1989 .

[27]  T. D. Harris,et al.  Breaking the Diffraction Barrier: Optical Microscopy on a Nanometric Scale , 1991, Science.

[28]  R. Kopelman,et al.  Submicrometer intracellular chemical optical fiber sensors. , 1992, Science.

[29]  R. Kopelman,et al.  High-performance fiber-optic pH microsensors for practical physiological measurements using a dual-emission sensitive dye. , 1997, Analytical chemistry.

[30]  R Kopelman,et al.  Development and cellular applications of fiber optic nitric oxide sensors based on a gold-adsorbed fluorophore. , 1998, Analytical chemistry.

[31]  Gerhard Heinzmann,et al.  Development of a submicron optochemical potassium sensor with enhanced stability due to internal reference , 1998 .

[32]  J. Harris.  Electron microscopy 1982, Vol. 3: biology (papers presented at the 10th international congress on electron microscopy) , 1983 .

[33]  R. Kopelman,et al.  Miniaturized fiber-optic chemical sensors with fluorescent dye-doped polymers , 1995 .

[34]  Tuan Vo-Dinh,et al.  Antibody-based nanoprobe for measurement of a fluorescent analyte in a single cell , 2000, Nature Biotechnology.

[35]  M. Shortreed,et al.  Fluorescent fiber-optic calcium sensor for physiological measurements. , 1996, Analytical chemistry.

[36]  T. Meyer,et al.  Fiber-optic nitric oxide-selective biosensors and nanosensors. , 1998, Analytical chemistry.

[37]  T. Vo‐Dinh.  Nanobiosensors: Probing the sanctuary of individual living cells , 2002, Journal of cellular biochemistry. Supplement.

[38]  Q. Peng,et al.  Apoptosis and necrosis induced with light and 5-aminolaevulinic acid-derived protoporphyrin IX. , 1996, British Journal of Cancer.

[39]  T. Vo‐Dinh,et al.  Biosensors and biochips: advances in biological and medical diagnostics , 2000, Fresenius' journal of analytical chemistry.