Study on Purification and Activity of Plasma Membrane H~+-ATPase of Populus euphratica .
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The plasma membrane from suspended cultured Populus euphratica (PE) cells had been isolated and purified by two phase partition composed of Dextran T 500 and PEG 3350. Effect of different concentrations of the polymer (5 5%,5 7%,5 9%,6 1%,6 3%,6 5%) and KCl (0,5,10,15 mmol/L) on the purification were examined. The results indicated that two phase system composed of 5 9% polymer without salt resulted in plasma membrane of PE with higher H + ATPase activity. The activity of the membrane H + ATPase has been increased 8 fold (from 4 23 to 32 63 μmol/mg·h) with better orientation and most of the membrane H + ATPases are right side out. These results may provide better basis and availability for further studies of the purified H + ATPase from PE plasma membrane.