Imaging of Calcium Oscillation in Mouse Oocyte/zygote by Two Photon Laser Scanning Microscopy

Calcium is one of the most important and versatile intracellular messenger, known as "the signal of life and death ". It has been found that, at certain time in the cell cycle, there is an oscillation of Ca2+ and its transmission (the Calcium wave) in the cell. Some useful information can be got from this phenomenon, like the speed of metabolism and the activity of the cell, ect. Using two photon laser scanning microscopy (TPLSM) system, we observed the dynamic process of the Ca2+ oscillation in mouse oocyte and zygote, obtaining the accurate cycle and amplitude of the oscillation. Then we successfully applied the results to the animal model experiment on hyperglycemia mice, with comparison to that on normal ones.