Broad spectrum antimicrobial activity of functionalized polyanilines.

The antimicrobial properties of conductive functionalized polyanilines (fPANI) were investigated by exploring their interaction with bacterial cells. In sharp contrast to polyaniline (PANI), lower concentrations of fPANI were needed to strongly inhibit the growth of wild-type Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus, as well as several antibiotic-resistant clinical pathogens. To gain an insight into how fPANI have an impact on cellular physiology we used a whole genome expression study in the model E. coli MG1655 strain exposed to a representative fPANI. The expression levels of 218 (∼5.1%) genes changed significantly. Moreover, we found that certain oxidative damage-responsive genes were strongly induced, while genes potentially involved in energy metabolism and transport and in forming bacterial cell walls and stress-resistant cellular communities (biofilms) were repressed. Taken together, our results appear to indicate that the antimicrobial effects of fPANI, in part at least, might stem from their ability to target the operations of multiple and diverse cellular processes, and suggest that fPANI could be useful ingredients for biomaterials used in the development of food packaging and medical devices.

[1]  A. Heeger.  Semiconducting and Metallic Polymers: The Fourth Generation of Polymeric Materials (Nobel Lecture) Copyright(c) The Nobel Foundation 2001. We thank the Nobel Foundation, Stockholm, for permission to print this lecture. , 2001, Angewandte Chemie.

[2]  M. Hande,et al.  Cytotoxicity and genotoxicity of silver nanoparticles in human cells. , 2009, ACS nano.

[3]  J. Imlay,et al.  Pathways of oxidative damage. , 2003, Annual review of microbiology.

[4]  J. Weiner,et al.  Effects of site-directed mutations in Escherichia coli succinate dehydrogenase on the enzyme activity and production of superoxide radicals. , 2006, Biochemistry and cell biology = Biochimie et biologie cellulaire.

[5]  T. Silhavy,et al.  Tethering of CpxP to the inner membrane prevents spheroplast induction of the Cpx envelope stress response , 2000, Molecular microbiology.

[6]  Robert A. LaRossa,et al.  DNA Microarray-Mediated Transcriptional Profiling of the Escherichia coli Response to Hydrogen Peroxide , 2001, Journal of bacteriology.

[7]  Sourabh Shukla,et al.  Sonochemical coating of paper by microbiocidal silver nanoparticles. , 2011, Langmuir : the ACS journal of surfaces and colloids.

[8]  M. Gizdavic-Nikolaidis,et al.  Electrospun functionalized polyaniline copolymer-based nanofibers with potential application in tissue engineering. , 2010, Macromolecular bioscience.

[9]  Chao Sun,et al.  Antibacterial Effect of the Conducting Polyaniline , 2009 .

[10]  A. Heeger,et al.  Synthesis of electrically conducting organic polymers: halogen derivatives of polyacetylene, (CH)x , 1977 .

[11]  J. Travas-sejdic,et al.  Conducting polymers as free radical scavengers , 2004 .

[12]  S. Normark,et al.  Molecular Characterization of the Acid-Inducible asr Gene of Escherichia coli and Its Role in Acid Stress Response , 2003, Journal of bacteriology.

[13]  T. Yura,et al.  Sensitization of Escherichia coli cells to oxidative stress by deletion of the rpoH gene, which encodes the heat shock sigma factor , 1992, Journal of bacteriology.

[14]  A. Heeger,et al.  Counter-ion induced processibility of conducting polyaniline and of conducting polyblends of polyaniline in bulk polymers , 1992 .

[15]  C. Soeller,et al.  Conducting polymers for electrochemical DNA sensing. , 2009, Biomaterials.

[16]  F. Blattner,et al.  Functional Genomics: Expression Analysis ofEscherichia coli Growing on Minimal and Rich Media , 1999, Journal of bacteriology.

[17]  M. Leclerc,et al.  Synthesis and characterization of poly(alkylanilines) , 1989 .

[18]  Y. Shai,et al.  Mode of action of membrane active antimicrobial peptides. , 2002, Biopolymers.

[19]  D. T. Seshadri,et al.  Use of polyaniline as an antimicrobial agent in textiles , 2005 .

[20]  J. Imlay.  Cellular defenses against superoxide and hydrogen peroxide. , 2008, Annual review of biochemistry.

[21]  Arthur J. Epstein,et al.  'Synthetic metals': a novel role for organic polymers , 1989 .

[22]  Noriyuki Kuramoto,et al.  Synthesis of processable polyaniline complexed with anionic surfactant and its conducting blends in aqueous and organic system , 2000 .

[23]  Yen Wei,et al.  Electrospinning polyaniline-contained gelatin nanofibers for tissue engineering applications. , 2006, Biomaterials.

[24]  L. Hamoen,et al.  Membrane potential is important for bacterial cell division , 2010, Proceedings of the National Academy of Sciences.

[25]  M. Zanetti,et al.  Genome-Wide Transcriptional Profiling of the Escherichia coli Response to a Proline-Rich Antimicrobial Peptide , 2004, Antimicrobial Agents and Chemotherapy.

[26]  T. Wood,et al.  Temporal gene-expression in Escherichia coli K-12 biofilms. , 2007, Environmental microbiology.

[27]  B. D. Malhotra,et al.  Synthesis and characterization of poly(aniline-co-o-anisidine) : a processable conducting copolymer , 1993 .

[28]  A. Heeger,et al.  Semiconducting and Metallic Polymers: The Fourth Generation of Polymeric Materials , 2001, Angewandte Chemie.

[29]  D. Sathyanarayana,et al.  Synthesis of electrically conducting copolymers of aniline with o/m-amino benzoic acid by an inverse emulsion pathway , 2002 .

[30]  Y. Furukawa.  Electronic absorption and vibrational spectroscopies of conjugated conducting polymers , 1996 .

[31]  D. Botstein,et al.  DNA microarray analysis of gene expression in response to physiological and genetic changes that affect tryptophan metabolism in Escherichia coli. , 2000, Proceedings of the National Academy of Sciences of the United States of America.

[32]  Alison M. Cupples,et al.  Triclocarban and triclosan biodegradation at field concentrations and the resulting leaching potentials in three agricultural soils. , 2010, Chemosphere.

[33]  Arthur J. Epstein,et al.  Synthesis of self-doped conducting polyaniline , 1990 .

[34]  A. Pron,et al.  Conducting blends of polyaniline with conventional polymers , 1997 .

[35]  Jadranka Travas-Sejdic,et al.  The antioxidant activity of conducting polymers in biomedical applications , 2004 .

[36]  M. Gizdavic-Nikolaidis,et al.  Chemical synthesis and characterization of poly(aniline‐co‐ethyl 3‐aminobenzoate) copolymers , 2010 .

[37]  T. Wood,et al.  Insights on Escherichia coli biofilm formation and inhibition from whole-transcriptome profiling. , 2009, Environmental microbiology.