Optical biosensors based on Prussian Blue films.

Optical biosensing schemes based on enzymatically modified inorganic/organic transparent films predominately composed of Prussian Blue are demonstrated. The composite film, which is non-electrochemically deposited on a non-conducting support. is used as an optical transducer for flow-through biosensors based on hydrolases and oxidases. Urease and glucose oxidase are utilized as model enzymes. Action of the urea biosensor is based on optical pH sensitivity of Prussian Blue indicator. The glucose biosensor is acting as first-generation optical biosensor based on in situ generated Prussian White transducer for hydrogen peroxide. These simple, single-pass transmission optical biosensors exhibit sensitivity in the millimolar range of concentration. The biosensors are very stable owing to presence of a poly(pyrrolylbenzoic acid) network in the composite material. This organic polymer plays a dual role as a binding agent for inorganic material and as a functionalized support for strong covalent immobilization of enzyme molecules.

[1]  R. Koncki,et al.  Optical sensing schemes for Prussian Blue/Prussian White film system , 2000 .

[2]  Marijana Jukić,et al.  Rapid Determination of Oxalate by an Amperometric Oxalate Oxidase‐Based Electrode , 2000 .

[3]  A. Malinauskas,et al.  Glucose biosensor based on glucose oxidase immobilized in electropolymerized polypyrrole and poly(o-phenylenediamine) films on a Prussian Blue-modified electrode , 2000 .

[4]  L. Gorton,et al.  Amperometric biosensor for glutamate using prussian blue-based "artificial peroxidase" as a transducer for hydrogen peroxide. , 2000, Analytical chemistry.

[5]  R. Koncki,et al.  Urea determination using pH-enzyme electrode. , 1999, Journal of pharmaceutical and biomedical analysis.

[6]  A. Karyakin,et al.  Prussian Blue-based `artificial peroxidase' as a transducer for hydrogen peroxide detection. Application to biosensors , 1999 .

[7]  Ursula E. Spichiger,et al.  Glucose Nanosensor Based on Prussian-Blue Modified Carbon-Fiber Cone Nanoelectrode and an Integrated Reference Electrode , 1999 .

[8]  Joseph Wang,et al.  Glucose microsensors based on carbon paste enzyme electrodes modified with cupric hexacyanoferrate , 1999 .

[9]  W. Shih,et al.  Chromium hexacyanoferrate based glucose biosensor , 1999 .

[10]  S. Dong,et al.  Cobalt(II)hexacyanoferrate film modified glassy carbon electrode for construction of a glucose biosensor , 1999 .

[11]  S. Z. Weisz,et al.  Chemically derived prussian blue sol-gel composite thin films , 1999 .

[12]  R. Koncki,et al.  Composite films of Prussian blue and N-substituted polypyrroles: covalent immobilization of enzymes and application to near infrared optical biosensing. , 1999, Biosensors & bioelectronics.

[13]  Lo Gorton,et al.  The electrocatalytic activity of Prussian blue in hydrogen peroxide reduction studied using a wall-jet electrode with continuous flow , 1998 .

[14]  R. Koncki,et al.  Composite Films of Prussian Blue and N-Substituted Polypyrroles:  Fabrication and Application to Optical Determination of pH. , 1998, Analytical chemistry.

[15]  S. Dong,et al.  Self-gelatinizable copolymer immobilized glucose biosensor based on prussian blue modified graphite electrode. , 1998, The Analyst.

[16]  B. Grabaric,et al.  Glucose determination in blood samples using flow injection analysis and an amperometric biosensor based on glucose oxidase immobilized on hexacyanoferrate modified nickel electrode , 1997 .

[17]  C. Cai,et al.  Amperometric biosensor for ethanol based on immobilization of alcohol dehydrogenase on a nickel hexacyanoferrate modified microband gold electrode. , 1997, Talanta.

[18]  L. Gorton,et al.  Prussian-Blue-based amperometric biosensors in flow-injection analysis. , 1996, Talanta.

[19]  Otto S. Wolfbeis,et al.  Capillary waveguide sensors , 1996 .

[20]  W. Pietro,et al.  Redox coenzyme functionalization of electrochemically grown Prussian blue films , 1996 .

[21]  A. Karyakin,et al.  Prussian Blue-Based First-Generation Biosensor. A Sensitive Amperometric Electrode for Glucose , 1995 .

[22]  Shaojun Dong,et al.  Amperometric biosensors based on the immobilization of oxidases in a Prussian blue film by electrochemical codeposition , 1995 .

[23]  Miguel Valcárcel,et al.  Flow-through (bio)chemical sensors—Plenary lecture , 1993 .

[24]  R. Koncki,et al.  Kinetic model of pH-based potentiometric enzymic sensors. Part 3. Experimental verification , 1992 .

[25]  H. B.F.,et al.  Catalytic oxidation of reduced nicotinamide adenine dinucleotide at hexacyanoferrate-modified nickel electrodes , 1987 .

[26]  Kingo Itaya,et al.  Catalysis of the reduction of molecular oxygen to water at Prussian blue modified electrodes , 1984 .

[27]  C. Burtis,et al.  A coupled-enzyme equilibrium method for measuring urea in serum: optimization and evaluation of the AACC study group on urea candidate reference method. , 1980, Clinical chemistry.

[28]  P. Trinder,et al.  An improved colour reagent for the determination of blood glucose by the oxidase system. , 1972, The Analyst.