Epidemiology and antibiotic sensitivity of Staphylococcus aureus nasal carriage in children in Hungary.

The aim of this study was to assess the Staphylococcus aureus nasal carriage rate in healthy children all over Hungary and to specify some risk factors, the antibiotic resistance patterns of the bacteria, and their genetic relatedness. In total, 878 children (aged 3-6 years) were screened at 21 day-care centers in 16 different cities in Hungary, between February 2009 and December 2011. Samples taken from both nostrils were cultured on blood agar, and suspected S. aureus isolates were identified by β-hemolysis, catalase positivity, clump test, and nucA PCR. Methicillin-resistant strains were screened by mecA and mecC PCR. Antibiotic susceptibility was determined by agar dilution or gradient test strips. Pulsed-field gel electrophoresis was used for genotyping. S. aureus carriage rate was found to be 21.3%, which correlates well with international data. We found no statistically significant correlation between the gender or the sibling status and S. aureus carriage. All isolates were sensitive to oxacillin, trimethoprim-sulfamethoxazole, and mupirocin. The resistance rates for erythromycin, ciprofloxacin, clindamycin, gentamicin, and tetracycline were 7.5%, 0.5%, 1.1%, 3.7%, and 4.3%, respectively. The isolates showed very high genetic diversity. In summary, carried S. aureus isolates are more sensitive to antibiotics compared with clinical isolates in Hungary, and methicillin-resistant S. aureus carriage rate is very low yet.

[1]  L. Jeremić,et al.  High Prevalence and Resistance Patterns of Community-Associated Methicillin-Resistant Staphylococcus Aureus in the Pomoravlje Region, Serbia. , 2016, Acta microbiologica et immunologica Hungarica.

[2]  A. van Belkum,et al.  Nasal carriers are more likely to acquire exogenous Staphylococcus aureus strains than non-carriers. , 2015, Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases.

[3]  S. Esposito,et al.  Oropharyngeal and nasal Staphylococcus aureus carriage by healthy children , 2014, BMC Infectious Diseases.

[4]  H. Goossens,et al.  Prevalence and resistance of commensal Staphylococcus aureus, including meticillin-resistant Staphylococcus aureus: a European cross-sectional study. , 2014 .

[5]  P. Horby,et al.  Staphylococcus aureus nasopharyngeal carriage in rural and urban northern Vietnam , 2014, Transactions of the Royal Society of Tropical Medicine and Hygiene.

[6]  A. Coran,et al.  Does Staphylococcusaureus nasal carriage require eradication prior to elective ambulatory surgery in children? , 2014, Pediatric Surgery International.

[7]  S. Shouche,et al.  Prevalence and risk factors for nasal carriage of Staphylococcus aureus in children attending anganwaries (preschools) in Ujjain, India , 2013, BMC Research Notes.

[8]  Herman Goossens,et al.  Prevalence and resistance of commensal Staphylococcus aureus, including meticillin-resistant S aureus, in nine European countries: a cross-sectional study. , 2013, The Lancet. Infectious diseases.

[9]  J. Parkhill,et al.  The newly described mecA homologue, mecALGA251, is present in methicillin-resistant Staphylococcus aureus isolates from a diverse range of host species. , 2012, The Journal of antimicrobial chemotherapy.

[10]  P. Lacor,et al.  COMMUNITY ASSOCIATED METHICILLINRESISTANT STAPHYLOCOCCUS AUREUS , 2012, Acta clinica Belgica.

[11]  L. Van Melderen,et al.  Differences in nasopharyngeal bacterial carriage in preschool children from different socio-economic origins. , 2011, Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases.

[12]  A. van Belkum,et al.  Methicillin-susceptible Staphylococcus aureus from clinical and community sources are genetically diverse. , 2011, International journal of medical microbiology : IJMM.

[13]  K. Nagy,et al.  Detection of Staphylococcus aureus nasal carriage in healthy young adults from a Hungarian University. , 2011, Acta microbiologica et immunologica Hungarica.

[14]  A. Pathak,et al.  Nasal Carriage and Antimicrobial Susceptibility of Staphylococcus aureus in healthy preschool children in Ujjain, India , 2010, BMC pediatrics.

[15]  F. DeLeo,et al.  Community-associated meticillin-resistant Staphylococcus aureus , 2010, The Lancet.

[16]  Anindita Das,et al.  A community-based study on nasal carriage of Staphylococcus aureus. , 2009, The Indian journal of medical research.

[17]  M. Lipsitch,et al.  Epidemiology and risk factors for Staphylococcus aureus colonization in children in the post-PCV7 era , 2009, BMC infectious diseases.

[18]  J. Szabó,et al.  Phenotypic and genetic characterisation of methicillin-resistant Staphylococcus aureus strains isolated from the university hospitals of Debrecen , 2009, European Journal of Clinical Microbiology & Infectious Diseases.

[19]  Z. Baykan,et al.  Nasal carriage of Staphylococcus aureus in healthy preschool children. , 2008, Japanese journal of infectious diseases.

[20]  Alex van Belkum,et al.  The role of nasal carriage in Staphylococcus aureus infections. , 2005, The Lancet. Infectious diseases.

[21]  H. Wertheim,et al.  Nasal Carriage of Staphylococcus aureus and Prevention of Nosocomial Infections , 2005, Infection.

[22]  Y. Carmeli,et al.  Association between carriage of Streptococcus pneumoniae and Staphylococcus aureus in Children. , 2004, JAMA.

[23]  S. Bassetti,et al.  "Cloud" health-care workers. , 2001, Emerging infectious diseases.

[24]  H. Eichenwald,et al.  The "cloud baby": an example of bacterial-viral interaction. , 1960, American journal of diseases of children.

[25]  Richa Katiyar,et al.  Prevalence of multidrug-resistant, coagulase-positive Staphylococcus aureus in nasal carriage, food, wastewater and paper currency in Jalandhar city (north-western), an Indian state of Punjab , 2014, Environmental Monitoring and Assessment.