论文信息 - Targeted DNA transfection into the mouse central nervous system using laser-induced stress waves.

Targeted DNA transfection into the mouse central nervous system using laser-induced stress waves.

We investigated the feasibility of gene transfer into the mouse central nervous system (CNS) by applying nanosecond pulsed laser-induced stress waves (LISWs). Intraventricular or hippocampal injection of a reporter gene [enhanced green fluorescent protein (EGFP)] followed by application of LISWs showed this method to be efficient in the CNS of newborn and adult mice. Cells expressing EGFP reside at least 3.5 mm from the surface of the tissue, while no apparent damage was detected. Additionally, expression of EGFP was limited to the area that was exposed to LISWs. Using this method, the formulation of plasmid DNA by cationic transfer reagent polyethylenimine proved to be effective for improving transfer efficiency into the CNS.

[1] M J Jaroszeski,et al. Theory and in vivo application of electroporative gene delivery. , 2000, Molecular therapy : the journal of the American Society of Gene Therapy.

[2] Yasuhiro Watanabe,et al. Neuroprotection by estrogen via extracellular signal‐regulated kinase against quinolinic acid‐induced cell death in the rat hippocampus , 2001, The European journal of neuroscience.

[3] Y. Taniyama,et al. Development of safe and efficient novel nonviral gene transfer using ultrasound: enhancement of transfection efficiency of naked plasmid DNA in skeletal muscle , 2002, Gene Therapy.

[4] Minoru Obara,et al. Gene transfer into mammalian cells by use of a nanosecond pulsed laser-induced stress wave. , 2004, Optics letters.

[5] Minoru Obara,et al. In vivo targeted gene transfer in skin by the use of laser‐induced stress waves , 2004, Lasers in surgery and medicine.

[6] G M Edelman,et al. Targeted DNA recombination in vivo using an adenovirus carrying the cre recombinase gene. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[7] G. Levi,et al. Size, diffusibility and transfection performance of linear PEI/DNA complexes in the mouse central nervous system , 1998, Gene Therapy.

[8] J. Darlix,et al. Efficient gene transfer in mouse neural precursors with a bicistronic retroviral vector , 2001, Journal of neuroscience research.