Facile synthesis of amino-silane modified superparamagnetic Fe3O4 nanoparticles and application for lipase immobilization.

In present study, a facile prepared nano-sized magnetic support was successfully synthesized. Then this support was applied for lipase immobilization using glutaraldehyde as a coupling agent. Experimental data showed that the immobilized lipase exhibited good thermal stability and reusability. The lipase loading amount and activity recovery were found to be 43.6 mg/g support and 58.2%. Kinetic studies suggested it an acceptable degree of specificity retention for an immobilization process.

[1]  A. Kiener,et al.  Industrial biocatalysis today and tomorrow , 2001, Nature.

[2]  O. Aktsipetrov.  Nonlinear magneto-optics in magnetic nanoparticles , 2002 .

[3]  Kim E. Mooney,et al.  Superparamagnetic Cobalt Ferrite Nanocrystals Synthesized by Alkalide Reduction , 2004 .

[4]  Huizhou Liu,et al.  Immobilization of lipase onto micron-size magnetic beads. , 2005, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[5]  Koichi Yamada,et al.  Isolation and Identification of Alkaline Lipase Producing Microorganisms, Cultural Conditions and Some Properties of Crude Enzymes , 1977 .

[6]  Zhiya Ma,et al.  Superparamagnetic silica nanoparticles with immobilized metal affinity ligands for protein adsorption , 2006 .

[7]  R. Verger,et al.  Lipases: Interfacial Enzymes with Attractive Applications. , 1998, Angewandte Chemie.

[8]  G. M. Pastor,et al.  Towards atomic-scale design: A theoretical investigation of magnetic nanoparticles and ultrathin films , 2008, Microelectron. J..

[9]  Yongxiao Bai,et al.  Study on immobilization of lipase onto magnetic microspheres with epoxy groups , 2009 .

[10]  A. Klibanov.  Improving enzymes by using them in organic solvents , 2001, Nature.

[11]  Abraham Ulman,et al.  Activity of Candida rugosa lipase immobilized on gamma-Fe2O3 magnetic nanoparticles. , 2003, Journal of the American Chemical Society.

[12]  Yongxiao Bai,et al.  Characterization of Candida rugosa lipase immobilized onto magnetic microspheres with hydrophilicity , 2008 .

[13]  Shengfu Wang,et al.  Carbon-coated nickel magnetic nanoparticles modified electrodes as a sensor for determination of acetaminophen , 2007 .

[14]  Yong Hu,et al.  Synthesis and magnetic properties of biocompatible hybrid hollow spheres. , 2006, Biomacromolecules.

[15]  M. Haas,et al.  Customizing lipases for biocatalysis: a survey of chemical, physical and molecular biological approaches , 2000 .

[16]  M. M. Bradford.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. , 1976, Analytical biochemistry.

[17]  Peter Dunnill,et al.  Non-porous magnetic chelator supports for protein recovery by immobilised metal affinity adsorption , 1996 .

[18]  B. Dijkstra,et al.  Bacterial biocatalysts: molecular biology, three-dimensional structures, and biotechnological applications of lipases. , 1999, Annual review of microbiology.

[19]  Chi‐Huey Wong,et al.  Enzymes for Chemical Synthesis , 2001 .