Physicochemical, microbiological and antimicrobial properties of commercial honeys from Portugal.

The present study aimed to characterize five commercial honeys available in the Portuguese market in respect to their floral origins, physicochemical parameters and microbial safety and commercial quality assessment. Pollen profile, colour, moisture content, ash, acidity, electrical conductivity, pH, reducing sugars, apparent sucrose and HMF were the parameters analysed in each honey sample. Aerobic mesophiles, moulds and yeasts, fecal coliforms and sulphite-reducing clostridia were the microbial contaminants of interest studied. The antimicrobial effect against four fermentative yeasts was determined. Concerning the physicochemical parameters, all honey samples were found to meet European Legislation (EC Directive 2001/110) for all parameters, except for HMF and apparent sucrose. Microbiologically, the commercial quality was considered good and all samples showed to be negative in respect to safety parameters. We also verified that the presence of honey differentially affected the growth of fermentative yeasts under study, depending on the type of yeast, but this growth was not significantly influenced by the type of honey used.

[1]  Pilar Buera,et al.  Pattern of pH and electrical conductivity upon honey dilution as a complementary tool for discriminating geographical origin of honeys , 2007 .

[2]  S. Muniategui,et al.  Evaluating soluble and insoluble ash, alkalinity of soluble and insoluble ash and total alkalinity of ash in honey using electrical conductivity measurements at 20 °C , 1992 .

[3]  W. Horwitz Official Methods of Analysis , 1980 .

[4]  D. Cliver,et al.  Microorganisms in honey. , 1996, International journal of food microbiology.

[5]  E. Crane Honey. A comprehensive survey , 1978, Medical History.

[6]  Stefan Bogdanov,et al.  Physico-chemical and bioactive properties of different floral origin honeys from Romania , 2009 .

[7]  M. Lindström,et al.  Prevalence and diversity of Clostridium botulinum types A, B, E and F in honey produced in the Nordic countries. , 2005, International journal of food microbiology.

[8]  F. Heredia,et al.  Characterisation of Moroccan unifloral honeys by their physicochemical characteristics , 2002 .

[9]  A. Al-Khalifa,et al.  Physicochemical characteristics and pollen spectrum of some Saudi honeys , 1999 .

[10]  G. Erdtman,et al.  Pollen morphology and plant taxonomy : an introduction to palynology , 1952 .

[11]  L.da C Azeredo,et al.  Protein contents and physicochemical properties in honey samples of Apis mellifera of different floral origins , 2003 .

[12]  A. Ferrer,et al.  Characterization of honey produced in Venezuela , 2004 .

[13]  M. Lindström,et al.  High prevalence of Clostridium botulinum types A and B in honey samples detected by polymerase chain reaction. , 2002, International journal of food microbiology.

[14]  J. Cantalapiedra,et al.  Pollen spectrum and physico-chemical attributes of heather (Erica sp.) honeys of north Portugal , 2009 .

[15]  R. Figueirêdo,et al.  Caracterização físico-química de méis produzidos no Estado do Piauí para diferentes floradas , 2004 .

[16]  H. Hooghiemstra [Review of: G. Erdtman (1986) Pollen morphology and plant taxonomy: Angiosperms (an introduction to palynology)] , 2009 .

[17]  G. Downey,et al.  Preliminary contribution to the characterisation of artisanal honey produced on the island of Ireland by palynological and physico-chemical data , 2005 .

[18]  Jonathan W. White The Composition of Honey , 1957 .

[19]  P. Andrade,et al.  Physicochemical attributes and pollen spectrum of Portuguese heather honeys , 1999 .

[20]  E. Oliveira,et al.  Caracterização físico-química de méis comercializados no município de Aracati-CE , 2011 .

[21]  Mónica S. Finola,et al.  Microbiological and chemical characterization of honeys from central Argentina , 2007 .

[22]  A. Verzera,et al.  The European Food Legislation and its impact on honey sector , 2006 .

[23]  Murat Küçük,et al.  Biological activities and chemical composition of three honeys of different types from Anatolia , 2007 .

[24]  R. Fritz,et al.  Characterization of microorganisms in Argentinean honeys from different sources. , 2005, International journal of food microbiology.

[25]  M. Al-Habori,et al.  Antioxidant activities and total phenolics of different types of honey , 2002 .