Using Various Skin Graft Techniques in Major Burn Reconstruction: A Lesson Learned From a Taiwanese Cornstarch Explosion.

PURPOSE Four hundred and ninety-nine patients had burn injuries in an explosion in Taiwan on June 27, 2015, 24 were admitted to the intensive care units of Taipei Veterans General Hospital. This study details our experience with surgical management of these patients, focusing primarily on various skin graft techniques. MATERIALS AND METHODS This single-center retrospective study included patients who underwent at least one of the previously mentioned skin graft techniques because of extensive skin defects. The demography, burn diagram, treatment modalities, postoperative outcome, and costs were all analyzed, and a comparison with traditional mesh skin grafts was performed. The literature was also reviewed. RESULTS Fourteen patients underwent the Meek skin graft technique. Only 3 received ReCell and 1 cultured epithelial autograft (CEA) at separate time point. Overall, the autologous skin grafts, including Meek/ReCell/CEA were completed within 6 months. The average skin graft success rate was approximately 72.9%, 79.2%, and 38% in Meek, ReCell, and CEA, respectively. The infection rate was approximately 35.7%, 25%, and 100% in Meek, ReCell, and CEA, respectively. The average surgical cost and total medical cost were significantly higher in patients who underwent Meek/ReCell/CEA treatments. CONCLUSIONS In our experience, Meek and ReCell treatments had acceptable success rates, but CEA treatment not. ReCell and CEA treatments are useful in the event of extremely limited donor sites, and they are fragile, easily infected, and technically challenging. These techniques also require longer hospitalization and tend to be more expensive, all factors that should be considered when assessing treatment options.

[1]  M. Ninkovic,et al.  The MEEK technique: 10‐year experience at a tertiary burn centre , 2017, International wound journal.

[2]  W. Chun,et al.  The application of cultured epithelial autografts improves survival in burns , 2015, Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society.

[3]  K. Busch,et al.  Combination of Medical Needling and ReCell ® for Repigmentation of Hypopigmented Burn Scars , 2014 .

[4]  O. Damour,et al.  Cultured allogenic keratinocytes for extensive burns: a retrospective study over 15 years. , 2014, Burns : journal of the International Society for Burn Injuries.

[5]  H. Matsumura,et al.  Chronological histological findings of cultured epidermal autograft over bilayer artificial dermis. , 2013, Burns : journal of the International Society for Burn Injuries.

[6]  Hag-Young Lee Outcomes of sprayed cultured epithelial autografts for full-thickness wounds: a single-centre experience. , 2012, Burns : journal of the International Society for Burn Injuries.

[7]  F. Wood,et al.  A prospective randomised clinical pilot study to compare the effectiveness of Biobrane® synthetic wound dressing, with or without autologous cell suspension, to the local standard treatment regimen in paediatric scald injuries. , 2012, Burns : journal of the International Society for Burn Injuries.

[8]  M. Fear,et al.  Characterisation of the cell suspension harvested from the dermal epidermal junction using a ReCell® kit. , 2012, Burns : journal of the International Society for Burn Injuries.

[9]  J. J. Coleman,et al.  Coverage of Large Pediatric Wounds With Cultured Epithelial Autografts in Congenital Nevi and Burns: Results and Technique , 2009, Journal of burn care & research : official publication of the American Burn Association.

[10]  L. Kamolz,et al.  Adult Burn Patients With More Than 60% TBSA Involved–Meek and Other Techniques to Overcome Restricted Skin Harvest Availability–The Viennese Concept , 2009, Journal of burn care & research : official publication of the American Burn Association.

[11]  Ted T. Huang,et al.  Five years' experience of the modified Meek technique in the management of extensive burns. , 2008, Burns : journal of the International Society for Burn Injuries.

[12]  B. De Angelis,et al.  A randomized trial comparing ReCell system of epidermal cells delivery versus classic skin grafts for the treatment of deep partial thickness burns. , 2007, Burns : journal of the International Society for Burn Injuries.

[13]  Fiona M Wood,et al.  The use of a non-cultured autologous cell suspension and Integra dermal regeneration template to repair full-thickness skin wounds in a porcine model: a one-step process. , 2007, Burns : journal of the International Society for Burn Injuries.

[14]  Ning Li,et al.  [Clinical application of Meek micrograft technique]. , 2007, Zhonghua yi xue za zhi.

[15]  F. Wood,et al.  The use of cultured epithelial autograft in the treatment of major burn injuries: a critical review of the literature. , 2006, Burns : journal of the International Society for Burn Injuries.

[16]  Cai-ping He,et al.  [Clinical application of Meek skin grafting technique]. , 2006, Nan fang yi ke da xue xue bao = Journal of Southern Medical University.

[17]  Chung-Sheng Lai,et al.  "Flypaper technique" a modified expansion method for preparation of postage stamp autografts. , 2005, Burns : journal of the International Society for Burn Injuries.

[18]  R. Shelton,et al.  In vitro transfer of keratinocytes: comparison of transfer from fibrin membrane and delivery by aerosol spray. , 2005, Journal of biomedical materials research. Part B, Applied biomaterials.

[19]  P. Shakespeare Burn wound healing and skin substitutes. , 2001, Burns : journal of the International Society for Burn Injuries.

[20]  F. A. Navarro,et al.  Sprayed keratinocyte suspensions accelerate epidermal coverage in a porcine microwound model. , 2000, The Journal of burn care & rehabilitation.

[21]  H. Carsin,et al.  Cultured epithelial autografts in extensive burn coverage of severely traumatized patients: a five year single-center experience with 30 patients. , 2000, Burns : journal of the International Society for Burn Injuries.

[22]  A. Trivisonno,et al.  Micrografting in the treatment of severely burned patients. , 1997, Burns : journal of the International Society for Burn Injuries.

[23]  H. Green,et al.  Seria cultivation of strains of human epidemal keratinocytes: the formation keratinizin colonies from single cell is , 1975, Cell.

[24]  C. Meek Successful microdermagrafting using the Meek-Wall microdermatome. , 1958, American journal of surgery.

[25]  R. Salisbury Adult Burn Patients With More Than 60% TBSA Involved–Meek and Other Techniques to Overcome Restricted Skin Harvest Availability–The Viennese Concept , 2012 .

[26]  G. Germann,et al.  Experience with the modified Meek technique. , 1996, Acta chirurgiae plasticae.