Clinical significance of serum hydroxyproline‐containing peptides with special reference to hyproprotein

Abstract. Serum hydroxyproline‐containing peptides were separated into three fractions, i.e. large protein, polypeptide and smaller peptides, by gel filtration on Sephadex G‐200. The large protein was presumed to be the Clq subunit of the first component of complement by an immunological analysis. The polypeptide had an electrophoretic mobility corresponding to β‐globulin and its molecular weight was estimated between 35 000 and 45 000 by gel filtration analysis. The polypeptide might well be defined as hyproprotein. The molecular weight of smaller peptides was estimated between 1400 and 3000.