Detection of methanogenic archaea in seawater particles and the digestive tract of a marine fish species.

A methanogen-specific nested PCR approach was used to detect methanogenic archaea in seawater particles of the North Sea and the feces and the digestive tract of flounder (Platichthys flesus), a fish found in the North Sea. A number of 16S rDNA sequences with 97.6-99.5% similarity to Methanococcoides methylutens were found in the seawater particles as well as the digestive tract and fecal samples.

[1]  Rebekka R. E. Artz,et al.  University of Groningen Association of marine archaea with the digestive tracts of two marine fish species , 2017 .

[2]  Erko Stackebrandt,et al.  Taxonomic Note: A Place for DNA-DNA Reassociation and 16S rRNA Sequence Analysis in the Present Species Definition in Bacteriology , 1994 .

[3]  Cindy Lee,et al.  Methane production during zooplankton grazing on marine phytoplankton , 1994 .

[4]  D. Stahl,et al.  Group-specific 16S rRNA hybridization probes to describe natural communities of methanogens , 1994, Applied and environmental microbiology.

[5]  JMcN Sieburth.  C1 bacteria in the water column of Chesapeake Bay USA. I. Distribution of sub-populations of O2-tolerant, obligately anaerobic, methylotrophic methanogens that occur in microniches reduced by their bacterial consorts , 1993 .

[6]  D. Marty.  Methanogenic bacteria in seawater , 1993 .

[7]  A. Shanks,et al.  Reducing microzones and sulfide production in marine snow , 1993 .

[8]  E. Delong.  Archaea in coastal marine environments. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[9]  K. Schleifer,et al.  8 The Methanogenic Bacteria , 1992 .

[10]  A. Alldredge,et al.  Can Microscale Chemical Patches Persist in the Sea? Microelectrode Study of Marine Snow, Fecal Pellets , 1987, Science.

[11]  J. T. Staley,et al.  Baleen whales: preliminary evidence for forestomach microbial fermentation , 1984, Applied and environmental microbiology.

[12]  R. Oremland,et al.  Methanogenesis and sulfate reduction: competitive and noncompetitive substrates in estuarine sediments. , 1982, Applied and environmental microbiology.

[13]  R. Oremland.  Methanogenic activity in plankton samples and fish intestines A mechanism for in situ methanogenesis in oceanic surface waters , 1979 .