Fibre-optic biosensor based on luminescence and immobilized enzymes: microdetermination of sorbitol, ethanol and oxaloacetate.

We have investigated highly selective and ultrasensitive biosensors based on luminescent enzyme systems linked to optical transducers. A fibre-optic sensor with immobilized enzymes was designed; the solid-phase bioreagent was maintained in close contact contact with the tip of a glass fibre bundle connected to the photomultiplier tube of a luminometer. A bacterial luminescence fibre-optic sensor was used for the microdetermination of NADH. Various NAD(P)-dependent enzymes, sorbitol dehydrogenase, alcohol dehydrogenase and malate dehydrogenase, were co-immobilized on preactivated polyamide membranes with the bacterial system and used for the microdetermination of sorbitol, ethanol and oxaloacetate at the nanomolar level with a good precision.

[1]  W. Rudolf Seitz,et al.  Chemical Sensors Based on Fiber Optics , 1984 .

[2]  P. R. Coulet,et al.  New Immobilized Enzyme Membranes for Tailor-Made Biosensors , 1986 .

[3]  An Optical Fibre Probe for the Determination of Glucose Based on Immobilized Glucose Dehydrogenase , 1988 .

[4]  W. Rudolf Seitz,et al.  Chemiluminescence fiber optic probe for hydrogen peroxide based on the luminol reaction , 1978 .

[5]  M. Aizawa,et al.  Photovoltaic Determination of Hydrogen Peroxide with A Biophotodiode , 1984 .

[6]  L. Blum,et al.  Fibre-optic sensor with co-immobilised bacterial bioluminescence enzymes. , 1989, Biosensors.

[7]  Pierre R. Coulet,et al.  Chemiluminescent Analyte Microdetection Based on the Luminol-H2 O 2 Reaction Using Peroxidase Immobilized on New Synthetic Membranes , 1987 .

[8]  M A Arnold,et al.  Enzyme-based fiber optic sensor. , 1985, Analytical chemistry.

[9]  P. R. Coulet,et al.  Luminescence Fiber-Optic Biosensor , 1988 .

[10]  Michael J. Sepaniak,et al.  Chemical Sensors Based on Immobilized Indicators and Fiber Optics , 1988 .

[11]  D W Lübbers,et al.  Influence of enzyme concentration and thickness of the enzyme layer on the calibration curve of the continuously measuring glucose optode. , 1984, Advances in experimental medicine and biology.

[12]  G D Christian,et al.  Single fiber-optic fluorescence enzyme-based sensor. , 1988, Analytical chemistry.

[13]  O. V. Lebedeva,et al.  Immobilized bacterial luciferase and its applications , 1987, Applied biochemistry and biotechnology.

[14]  L. Blum,et al.  Collagen strip with immobilized luciferase for ATP bioluminescent determination , 1985, Biotechnology and bioengineering.

[15]  Optical sensors for clinical applications , 1987 .

[16]  O. Wolfbeis.  Fiber-optic probe for kinetic determination of enzyme activities. , 1986, Analytical chemistry.

[17]  L. Blum,et al.  Immobilized Biological Compounds in Bio- and Chemiluminescence Assays , 1988 .

[18]  L. Blum,et al.  Atypical kinetics of immobilized firefly luciferase , 1986, Biotechnology and bioengineering.

[19]  O. Wolfbeis,et al.  Fibre-optic sensors in biomedical sciences , 1987 .

[20]  M A Arnold,et al.  Fiber-optic biosensors based on the fluorometric detection of reduced nicotinamide adenine dinucleotide. , 1988, Analytical chemistry.

[21]  D R Walt,et al.  Polymer immobilized enzyme optrodes for the detection of penicillin. , 1987, Analytical chemistry.