Suppression of Bulk Fluorescence Noise by Combining Waveguide-Based Near-Field Excitation and Collection

A high surface to bulk fluorescence ratio is very useful in bioimaging, sensing, sequencing, and physical chemistry characterization. We used the evanescent field of a photonic waveguide for highly localized excitation and collection of molecular fluorescence. As both near-field excitation and collection are strongly distance dependent, we were able to increase the surface to bulk fluorescence ratio significantly. We have also experimentally investigated the combined excitation and collection efficiency as a function of the position of the molecule in the near field. Finally, we formulated and experimentally verified a general condition for the waveguide–molecule interaction length for maximum optical efficiency of the device.

[1]  I. V. Paribok,et al.  Local modification of the silicon surface with protein molecules , 2007 .

[2]  Lorenzo Pavesi,et al.  Evanescent-field excitation and collection approach for waveguide based photonic luminescent biosensors , 2014 .

[3]  Ankur Gupta,et al.  Resonant plasmonic enhancement of single-molecule fluorescence by individual gold nanorods. , 2014, ACS nano.

[4]  D. Astruc,et al.  The copper(I)-catalyzed alkyne-azide cycloaddition (CuAAC) “click” reaction and its applications. An overview , 2011 .

[5]  Aaron S. Anderson,et al.  Waveguide-Based Biosensors for Pathogen Detection , 2009, Sensors.

[6]  L. Gunnarsson,et al.  Ultrahigh sensitivity made simple: nanoplasmonic label-free biosensing with an extremely low limit-of-detection for bacterial and cancer diagnostics , 2009, Nanotechnology.

[7]  S. Turner,et al.  Zero-Mode Waveguides for Single-Molecule Analysis at High Concentrations , 2003, Science.

[8]  Richard A. Keller,et al.  Single Molecule Detection in Solution , 2002 .

[9]  M. A. Otte,et al.  Trends and challenges of refractometric nanoplasmonic biosensors: a review. , 2014, Analytica chimica acta.

[10]  L. Liz‐Marzán,et al.  Sensing using plasmonic nanostructures and nanoparticles , 2015, Nanotechnology.

[11]  Roel Baets,et al.  Nanophotonic Waveguide Enhanced Raman Spectroscopy of Biological Submonolayers , 2016 .

[12]  N. Thompson,et al.  Measuring surface dynamics of biomolecules by total internal reflection fluorescence with photobleaching recovery or correlation spectroscopy. , 1981, Biophysical journal.

[13]  Zongfu Yu,et al.  Large single-molecule fluorescence enhancements produced by a bowtie nanoantenna , 2009 .

[14]  Jie He,et al.  Localized Surface Plasmon Resonance Biosensing: Current Challenges and Approaches , 2015, Sensors.

[15]  H. Rigneault,et al.  Photonic Methods to Enhance Fluorescence Correlation Spectroscopy and Single Molecule Fluorescence Detection , 2010, International journal of molecular sciences.

[16]  R. Baets,et al.  Development of a CMOS compatible biophotonics platform based on SiN nanophotonic waveguides , 2014, 2014 Conference on Lasers and Electro-Optics (CLEO) - Laser Science to Photonic Applications.

[17]  Roel Baets,et al.  Evanescent excitation and collection of spontaneous Raman spectra using silicon nitride nanophotonic waveguides. , 2014, Optics letters.

[18]  R. Baets,et al.  Low-Loss Singlemode PECVD Silicon Nitride Photonic Wire Waveguides for 532–900 nm Wavelength Window Fabricated Within a CMOS Pilot Line , 2013, IEEE Photonics Journal.