A universal cloning method based on yeast homologous recombination that is simple, efficient, and versatile.

[1]  C. Bond,et al.  Structure of the heterodimer of human NONO and paraspeckle protein component 1 and analysis of its role in subnuclear body formation , 2012, Proceedings of the National Academy of Sciences.

[2]  W. Edelmann,et al.  SLiCE: a novel bacterial cell extract-based DNA cloning method , 2012, Nucleic acids research.

[3]  Jingdong Tian,et al.  Circular Polymerase Extension Cloning of Complex Gene Libraries and Pathways , 2009, PloS one.

[4]  D. G. Gibson,et al.  Enzymatic assembly of DNA molecules up to several hundred kilobases , 2009, Nature Methods.

[5]  T. Luong,et al.  Improved single-copy integration vectors for Staphylococcus aureus. , 2007, Journal of microbiological methods.

[6]  S. Elledge,et al.  Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC , 2007, Nature Methods.

[7]  Christopher M. Crew,et al.  A high-throughput gene knockout procedure for Neurospora reveals functions for multiple transcription factors , 2006, Proceedings of the National Academy of Sciences.

[8]  G. O’Toole,et al.  Saccharomyces cerevisiae-Based Molecular Tool Kit for Manipulation of Genes from Gram-Negative Bacteria , 2006, Applied and Environmental Microbiology.

[9]  S. Carr,et al.  The srhSR gene pair from Staphylococcus aureus: genomic and proteomic approaches to the identification and characterization of gene function. , 2001, Biochemistry.

[10]  J. McCormick,et al.  Identification of a Novel Two-Component Regulatory System That Acts in Global Regulation of Virulence Factors ofStaphylococcus aureus , 2001, Journal of bacteriology.

[11]  Fred Winston,et al.  Construction of a set of convenient saccharomyces cerevisiae strains that are isogenic to S288C , 1995, Yeast.

[12]  S. Ho,et al.  Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension. , 1989, Gene.

[13]  K. Oldenburg,et al.  Recombination-mediated PCR-directed plasmid construction in vivo in yeast. , 1997, Nucleic acids research.

[14]  D. Botstein,et al.  Plasmid construction by homologous recombination in yeast. , 1987, Gene.