Single-cell microinjection technology in cell biology.

Single-cell microinjection has been successfully used to deliver exogenous proteins, cDNA constructs, peptides, drugs and particles into transfection-challenged cells. With precisely controlled delivery dosage and timing, microinjection has been used in many studies of primary cultured cells, transgenic animal production, in vitro fertilization and RNA inference. This review discusses the advantages and limits of microinjection as a mechanical delivery method and its applications to attached and suspended cells.

[1]  M. Fabbrini,et al.  Exogenous Protein Expression in Xenopus Oocytes , 2007 .

[2]  K. Rohr,et al.  Surveillance of siRNA integrity by FRET imaging , 2007, Nucleic acids research.

[3]  G. Novelli,et al.  Transfer and expression of foreign genes in mammalian cells. , 2000, BioTechniques.

[4]  Uwe Pliquett,et al.  Nonlinear current-voltage relationship of the plasma membrane of single CHO cells. , 2007, Bioelectrochemistry.

[5]  A. LeBlanc,et al.  Estrogen and Androgen Protection of Human Neurons against Intracellular Amyloid β1-42 Toxicity through Heat Shock Protein 70 , 2004, The Journal of Neuroscience.

[6]  E. Neumann,et al.  Gene transfer into mouse lyoma cells by electroporation in high electric fields. , 1982, The EMBO journal.

[7]  D. Goldfarb,et al.  Nuclear transport of RNAs in microinjected Xenopus oocytes. , 1998, Methods in cell biology.

[8]  W. Erl,et al.  Suppression of endothelial adhesion molecule up‐regulation with cyclopentenone prostaglandins is dissociated from IκB kinase inhibition and cell death induction , 2003 .

[9]  A. LeBlanc,et al.  Selective cytotoxicity of intracellular amyloid β peptide1–42 through p53 and Bax in cultured primary human neurons , 2002, The Journal of cell biology.

[10]  L. Mir,et al.  Cell electropermeabilization: a new tool for biochemical and pharmacological studies. , 1993, Biochimica et biophysica acta.

[11]  G. Kreitzer,et al.  Polarization-dependent selective transport to the apical membrane by KIF5B in MDCK cells. , 2007, Developmental cell.

[12]  S. Albrecht,et al.  Active caspase-6 and caspase-6-cleaved tau in neuropil threads, neuritic plaques, and neurofibrillary tangles of Alzheimer's disease. , 2004, The American journal of pathology.

[13]  P. Wadsworth.  Microinjection of mitotic cells. , 1999, Methods in cell biology.

[14]  A. Smit,et al.  Synapse Formation between Central Neurons Requires Postsynaptic Expression of the MEN1 Tumor Suppressor Gene , 2001, The Journal of Neuroscience.

[15]  R. Tuan,et al.  Electric field-induced molecular vibration for noninvasive, high-efficiency DNA transfection. , 2004, Molecular therapy : the journal of the American Society of Gene Therapy.

[16]  N. Nakatsuji,et al.  Electroporation of cynomolgus monkey embryonic stem cells , 2003, Genesis.

[17]  X. Roucou,et al.  p75 Neurotrophin Receptor Protects Primary Cultures of Human Neurons against Extracellular Amyloid β Peptide Cytotoxicity , 2003, The Journal of Neuroscience.

[18]  John Yu,et al.  Human TRIM71 and its nematode homologue are targets of let-7 microRNA and its zebrafish orthologue is essential for development. , 2007, Molecular biology and evolution.

[19]  H. O’Hagan,et al.  RPA and ATR link transcriptional stress to p53 , 2007, Proceedings of the National Academy of Sciences.

[20]  G. Augustine,et al.  A versatile microporation technique for the transfection of cultured CNS neurons , 1999, Journal of Neuroscience Methods.

[21]  C. Darlington,et al.  Damage to the vestibular inner ear causes long-term changes in neuronal nitric oxide synthase expression in the rat hippocampus , 2001, Neuroscience.

[22]  J. Thiery,et al.  Regulation of XSnail2 expression by Rho GTPases , 2007, Developmental dynamics : an official publication of the American Association of Anatomists.

[23]  X. Roucou,et al.  Cellular prion protein inhibits proapoptotic Bax conformational change in human neurons and in breast carcinoma MCF-7 cells , 2005, Cell Death and Differentiation.

[24]  R. Morgenstern,et al.  Efficient gene transfer into murine embryonic stem cells by nucleofection , 2004, Biotechnology Letters.

[25]  N. Kobayashi,et al.  Gene delivery to embryonic stem cells. , 2005, Birth defects research. Part C, Embryo today : reviews.

[26]  A. C. Perry,et al.  Piezo-actuated mouse intracytoplasmic sperm injection (ICSI) , 2007, Nature Protocols.

[27]  J. McGrath,et al.  Binding between particles and proteins in extracts: implications for microrheology and toxicity. , 2005, Acta biomaterialia.

[28]  J. Gehl,et al.  Electroporation: theory and methods, perspectives for drug delivery, gene therapy and research. , 2003, Acta physiologica Scandinavica.

[29]  Yu Sun,et al.  A Fully Automated Robotic System for Microinjection of Zebrafish Embryos , 2007, PloS one.

[30]  R. Kingston.  Introduction of DNA into Mammalian Cells , 2003 .

[31]  A. LeBlanc,et al.  Proteasomal degradation of caspase‐6 in 17β‐estradiol‐treated neurons , 2004, Journal of neurochemistry.

[32]  J. Borén,et al.  SNARE proteins mediate fusion between cytosolic lipid droplets and are implicated in insulin sensitivity , 2007, Nature Cell Biology.

[33]  A. LeBlanc,et al.  Prion Protein Protects Human Neurons against Bax-mediated Apoptosis* , 2001, The Journal of Biological Chemistry.

[34]  Kazuhiro Sudo,et al.  Efficient Transfection of Embryonic and Adult Stem Cells , 2004, Stem cells.

[35]  P. Labosky,et al.  Electroporation of Murine Embryonic Stem Cells: A Step‐by‐Step Guide , 2004, Stem cells.

[36]  X. Roucou,et al.  Cytosolic Prion Protein Is Not Toxic and Protects against Bax-mediated Cell Death in Human Primary Neurons* , 2003, Journal of Biological Chemistry.

[37]  M. Glogauer,et al.  Introduction of large molecules into viable fibroblasts by electroporation: optimization of loading and identification of labeled cellular compartments. , 1992, Experimental cell research.

[38]  R. Nusse,et al.  Creating transgenic Drosophila by microinjecting the site-specific φC31 integrase mRNA and a transgene-containing donor plasmid , 2007, Nature Protocols.

[39]  Y. Rojanasakul,et al.  Novel non-endocytic delivery of antisense oligonucleotides. , 2000, Advanced drug delivery reviews.

[40]  M. Kiefmann,et al.  Can ID Repetitive Elements Serve as Cis-acting Dendritic Targeting Elements? An In Vivo Study , 2007, PLoS ONE.

[41]  H. Guo,et al.  Caspase-1 activation of caspase-6 in human apoptotic neurons , 2006, Cell Death and Differentiation.

[42]  Yan Zhang,et al.  Selective and Protracted Apoptosis in Human Primary Neurons Microinjected with Active Caspase-3, -6, -7, and -8 , 2000, The Journal of Neuroscience.

[43]  B. Lebleu,et al.  Intracellular distribution of microinjected antisense oligonucleotides. , 1991, Proceedings of the National Academy of Sciences of the United States of America.