Raman imaging of changes in the polysaccharides distribution in the cell wall during apple fruit development and senescence

AbstractMain conclusionDuring on-tree ripening, the pectin distribution changed from polydispersed in cell wall to cumulated in cell wall corners. During apple storage, the pectin distribution returned to evenly dispersed along the cell wall. The plant cell wall influences the texture properties of fruit tissue for example apples become softer during ripening and postharvest storage. This softening process is believed to be mainly connected with changes in the cell wall composition due to polysaccharides undergoing an enzymatic degradation. These changes in polysaccharides are currently mainly investigated via chemical analysis or monoclonal labeling. Here, we propose the application of Raman microscopy for evaluating the changes in the polysaccharide distribution in the cell wall of apples during both ripening and postharvest storage. The apples were harvested 1 month and 2 weeks before optimal harvest date as well as at the optimal harvest date. The apples harvested at optimal harvest date were stored for 3 months. The Raman maps, as well as the chemical analysis were obtained for each harvest date and after 1, 2 and 3 months of storage, respectively. The analysis of the Raman maps showed that the pectins in the middle lamella and primary cell wall undergo a degradation. The changes in cellulose and hemicellulose were less pronounced. These findings were confirmed by the chemical analysis results. During development changes of pectins from a polydispersed form in the cell walls to a cumulated form in cell wall corners could be observed. In contrast after 3 months of apple storage we could observe an substantial pectin decrease. The obtained results demonstrate that Raman chemical imaging might be a very useful tool for a first identification of compositional changes in plant tissue during their development. The great advantage Raman microspectroscopy offers is the simultaneous localization and identification of polysaccharides within the cell wall and plant tissue.

[1]  S. Sansavini,et al.  Apple and peach consumption habits across European countries , 2010, Appetite.

[2]  D. Brummell,et al.  Lower cell wall pectin solubilisation and galactose loss during early fruit development in apple (Malus x domestica) cultivar 'Scifresh' are associated with slower softening rate. , 2015, Journal of plant physiology.

[3]  T. Hamann,et al.  Plant cell wall integrity maintenance as an essential component of biotic stress response mechanisms , 2012, Front. Plant Sci..

[4]  F. Harker,et al.  Texture of fresh fruit , 2010 .

[5]  M. Quesada,et al.  Fruit softening and pectin disassembly: an overview of nanostructural pectin modifications assessed by atomic force microscopy. , 2014, Annals of botany.

[6]  A. Zdunek,et al.  Use of FT-IR Spectra and PCA to the Bulk Characterization of Cell Wall Residues of Fruits and Vegetables Along a Fraction Process , 2012, Food Biophysics.

[7]  P. Adams,et al.  Raman imaging of cell wall polymers in Arabidopsis thaliana. , 2010, Biochemical and biophysical research communications.

[8]  R. Schröder,et al.  Cell wall structures leading to cultivar differences in softening rates develop early during apple (Malus x domestica) fruit growth , 2013, BMC Plant Biology.

[9]  P. Adams,et al.  Label-free in situ imaging of lignification in the cell wall of low lignin transgenic Populus trichocarpa , 2009, Planta.

[10]  B. Nicolai,et al.  Pectin modifications and the role of pectin-degrading enzymes during postharvest softening of Jonagold apples. , 2014, Food chemistry.

[11]  Notburga Gierlinger,et al.  The potential of Raman microscopy and Raman imaging in plant research , 2007 .

[12]  Notburga Gierlinger,et al.  Insights into the chemical composition of Equisetum hyemale by high resolution Raman imaging , 2007, Planta.

[13]  C. Renard.  Variability in cell wall preparations: quantification and comparison of common methods , 2005 .

[14]  E. Mehinagic,et al.  Relationship between texture and pectin composition of two apple cultivars during storage , 2008 .

[15]  Artur Zdunek,et al.  Imaging of polysaccharides in the tomato cell wall with Raman microspectroscopy , 2014, Plant Methods.

[16]  A. Zdunek,et al.  Combining FT-IR spectroscopy and multivariate analysis for qualitative and quantitative analysis of the cell wall composition changes during apples development. , 2015, Carbohydrate polymers.

[17]  Jizhan Liu,et al.  Fruit biomechanics based on anatomy: a review , 2013 .

[18]  U. Agarwal,et al.  Raman imaging to investigate ultrastructure and composition of plant cell walls: distribution of lignin and cellulose in black spruce wood (Picea mariana) , 2006, Planta.

[19]  Jianwei Qin,et al.  Investigation of Raman chemical imaging for detection of lycopene changes in tomatoes during postharvest ripening , 2011 .

[20]  The stiffening of the cell walls observed during physiological softening of pears , 2015, Planta.

[21]  Maja Musse,et al.  Analysis of the dynamic mechanical properties of apple tissue and relationships with the intracellular water status, gas distribution, histological properties and chemical composition , 2015 .

[22]  J. Sweedler,et al.  Base-induced delignification of Miscanthus x giganteus studied by three-dimensional confocal Raman imaging. , 2010, Bioresource technology.

[23]  M. McCann,et al.  Cell-cell adhesion in fresh sugar-beet root parenchyma requires both pectin esters and calcium cross-links , 2006 .

[24]  A. Catesson,et al.  Cell plate development and delayed formation of the pectic middle lamella in root meristems , 1988, Protoplasma.

[25]  D. Curti,et al.  Physicochemical properties of cell wall materials from apple, kiwifruit and tomato , 2008 .

[26]  J. Cornelis,et al.  Impact of rice cultivar and organ on elemental composition of phytoliths and the release of bio-available silicon , 2014, Front. Plant Sci..

[27]  Notburga Gierlinger,et al.  Imaging of plant cell walls by confocal Raman microscopy , 2012, Nature Protocols.

[28]  Jürgen Popp,et al.  Raman spectroscopic identification of single yeast cells , 2005 .

[29]  M. A. Strehle,et al.  On the way to a quality control of the essential oil of fennel by means of Raman spectroscopy , 2005, Biopolymers.

[30]  N. Gierlinger,et al.  Functional plant cell wall design revealed by the Raman imaging approach , 2010, Planta.

[31]  R. Tharanathan,et al.  Fruit Ripening Phenomena–An Overview , 2007, Critical reviews in food science and nutrition.

[32]  R. Volz,et al.  Firmness decline in 'Gala' apple during fruit development , 2003 .

[33]  Daniel J. Cosgrove,et al.  Comparative structure and biomechanics of plant primary and secondary cell walls , 2012, Front. Plant Sci..

[34]  Artur Zdunek,et al.  Study on dietary fibre by Fourier transform-infrared spectroscopy and chemometric methods. , 2016, Food chemistry.

[35]  N. Carpita,et al.  Structural models of primary cell walls in flowering plants: consistency of molecular structure with the physical properties of the walls during growth. , 1993, The Plant journal : for cell and molecular biology.

[36]  M. Knee,et al.  The chemistry of textural changes in fruit during storage , 1982 .

[37]  S. Ralph,et al.  FT-Raman Spectroscopy of Wood: Identifying Contributions of Lignin and Carbohydrate Polymers in the Spectrum of Black Spruce (Picea Mariana) , 1997 .

[38]  A. Zdunek,et al.  Evaluation of the Nanostructure of Pectin, Hemicellulose and Cellulose in the Cell Walls of Pears of Different Texture and Firmness , 2014, Food and Bioprocess Technology.

[39]  Da-Wen Sun,et al.  Modern Techniques for Food Authentication , 2008 .

[40]  Danny E. Akin,et al.  Near-infrared Fourier-transform Raman spectroscopy of flax (Linum usitatissimum L.) stems , 1998 .

[41]  A. Zdunek,et al.  The self-assembled network and physiological degradation of pectins in carrot cell walls , 2015 .

[42]  M. Jarvis,et al.  Intercellular adhesion and cell separation in plants , 2003 .

[43]  A. Synytsya.  Fourier transform Raman and infrared spectroscopy of pectins , 2003 .

[44]  Seth Debolt,et al.  Experimental approaches to study plant cell walls during plant-microbe interactions , 2014, Front. Plant Sci..

[45]  M. Schwanninger,et al.  Raman Imaging of Lignocellulosic Feedstock , 2013 .