Qualitative and Quantitative Determination of Caseins with Reverse‐Phase and Anion‐Exchange HPLC

Whole native caseinate (WNC) and casein (CN) fractions from preparative DE-52 cellulose urea columns were chromatographed using C-8 reverse-phase (RP) and DEAE-type anion-exchange (AEx) HPLC systems. With RP, αS2-CN and κ-CN eluted first as several small peaks; αS1-CN eluted later as two peaks, followed by β-CN peaks. With AEx, κ-CN eluted early as a group of peaks, β-CN eluted next, and αS1-CN and αS2-CN coeluted last. Standard curves were prepared for αS1-CN and β-CN using RP-PHLC and showed correlation coefficients of 0.99 and 0.98, respectively. The caseins in WNC, nonfat dry milk casein, commercial casein(ates) and caseins from milks of individual cows were determined.

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