Potential use of probiotic consortium isolated from kefir for textile azo dye decolorization.

Textile effluent treatment is challenging and has received considerable attention in recent decades. Textile effluents contain toxic substances, such as additives, detergents, surfactants, as well as synthetic dyes and their degradation by-products. Azo dyes are toxic, carcinogenic, mutagenic, and teratogenic to humans and to other life forms. In the present study, the decolorization and the degradation efficiency of Remazol brillant orange 3R (RBO 3R) was studied using a probiotic consortium (Lactobacillus acidophilus and Lactobacillus plantarum). Biodegradation of RBO 3R (750 ppm) was investigated under shaking condition in Mineral Salt Medium (MSM) solution at pH 11.5 and temperature 25°C. The dye degradation was further confirmed by FTIR and UV-Visible analysis. Under optimal conditions, the bacterial consortium was able to decolorize the dye completely (>99%) within 12 h. The color removal was 99.37% at 750 ppm. Muliplex PCR technique was utilized to detect the Lactobacillus genes. Using phytotoxicity, cytotoxicity, mutagenicity and biototoxicity end points. Toxicological studies of untreated (UT) and bacterial treated (BT) RBO 3R dye solutions were studied. Toxicity assay indicated that bacterial treatment led to detoxification of RBO 3R dye.

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