Perfusion in rat brain at 7 T with arterial spin labeling using FAIR-TrueFISP and QUIPSS.

Measurement of perfusion in longitudinal studies allows for the assessment of tissue integrity and the detection of subtle pathologies. In this work, the feasibility of measuring brain perfusion in rats with high spatial resolution using arterial spin labeling is reported. A flow-sensitive alternating recovery sequence, coupled with a balanced gradient fast imaging with steady-state precession readout section was used to minimize ghosting and geometric distortions, while achieving high signal-to-noise ratio. The quantitative imaging of perfusion using a single subtraction method was implemented to address the effects of variable transit delays between the labeling of spins and their arrival at the imaging slice. Studies in six rats at 7 T showed good perfusion contrast with minimal geometric distortion. The measured blood flow values of 152.5+/-6.3 ml/100 g per minute in gray matter and 72.3+/-14.0 ml/100 g per minute in white matter are in good agreement with previously reported values based on autoradiography, considered to be the gold standard.

[1]  Louise van der Weerd,et al.  Regional Variation of Cerebral Blood Flow and Arterial Transit Time in the Normal and Hypoperfused Rat Brain Measured Using Continuous Arterial Spin Labeling MRI , 2006, Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism.

[2]  E. Vaucher,et al.  Autoradiographic distribution of cerebral blood flow increases elicited by stimulation of the nucleus basalis magnocellularis in the unanesthetized rat , 1995, Brain Research.

[3]  X Golay,et al.  Non-invasive Measurement of Perfusion: a Critical Review of Arterial Spin Labelling Techniques , 2022 .

[4]  B. Rosen,et al.  Perfusion imaging with NMR contrast agents , 1990, Magnetic resonance in medicine.

[5]  U. Klose,et al.  FAIR‐TrueFISP imaging of cerebral perfusion in areas of high magnetic susceptibility differences at 1.5 and 3 Tesla , 2007, Journal of magnetic resonance imaging : JMRI.

[6]  P. Bandettini,et al.  QUIPSS II with thin‐slice TI1 periodic saturation: A method for improving accuracy of quantitative perfusion imaging using pulsed arterial spin labeling , 1999, Magnetic resonance in medicine.

[7]  Xavier Golay,et al.  Arterial spin labeling: benefits and pitfalls of high magnetic field. , 2006, Neuroimaging clinics of North America.

[8]  Geoff J M Parker,et al.  What levels of precision are achievable for quantification of perfusion and capillary permeability surface area product using ASL? , 2007, Magnetic resonance in medicine.

[9]  M. Griswold,et al.  A simple geometrical description of the TrueFISP ideal transient and steady‐state signal , 2006, Magnetic resonance in medicine.

[10]  R. Buxton,et al.  Quantitative imaging of perfusion using a single subtraction (QUIPSS and QUIPSS II) , 1998 .

[11]  S Warach,et al.  A general kinetic model for quantitative perfusion imaging with arterial spin labeling , 1998, Magnetic resonance in medicine.

[12]  R. Buxton,et al.  Implementation of quantitative perfusion imaging techniques for functional brain mapping using pulsed arterial spin labeling , 1997, NMR in biomedicine.

[13]  X. Golay,et al.  Arterial Spin Labeling: a One-stop-shop for Measurement of Brain Perfusion in the Clinical Settings , 2007, 2007 29th Annual International Conference of the IEEE Engineering in Medicine and Biology Society.

[14]  A. Nobre,et al.  Qualitative mapping of cerebral blood flow and functional localization with echo-planar MR imaging and signal targeting with alternating radio frequency. , 1994, Radiology.

[15]  Jeroen van der Grond,et al.  Brain perfusion territory imaging: methods and clinical applications of selective arterial spin-labeling MR imaging. , 2008, Radiology.

[16]  M. Raichle,et al.  What is the Correct Value for the Brain-Blood Partition Coefficient for Water? , 1985, Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism.

[17]  R R Edelman,et al.  Echo-planar MR imaging. , 1994, Radiology.

[18]  G. Pell,et al.  Pulsed arterial spin labeling using TurboFLASH with suppression of intravascular signal , 2003, Magnetic resonance in medicine.

[19]  E. Wong,et al.  Longitudinal MRI studies in the isoflurane‐anesthetized rat: long‐term effects of a short hypoxic episode on regulation of cerebral blood flow as assessed by pulsed arterial spin labelling , 2008, NMR in biomedicine.

[20]  Seong-Gi Kim.  Quantification of relative cerebral blood flow change by flow‐sensitive alternating inversion recovery (FAIR) technique: Application to functional mapping , 1995, Magnetic resonance in medicine.

[21]  K. Uğurbil,et al.  Determination of blood longitudinal relaxation time (T1) at high magnetic field strengths. , 2007, Magnetic resonance imaging.

[22]  S. Kety,et al.  THE NITROUS OXIDE METHOD FOR THE QUANTITATIVE DETERMINATION OF CEREBRAL BLOOD FLOW IN MAN: THEORY, PROCEDURE AND NORMAL VALUES. , 1948, The Journal of clinical investigation.

[23]  L R Schad,et al.  Pharmacokinetic parameters in CNS Gd-DTPA enhanced MR imaging. , 1991, Journal of computer assisted tomography.

[24]  U. Klose,et al.  FAIR true‐FISP perfusion imaging of the kidneys , 2004, Magnetic resonance in medicine.

[25]  D. S. Williams,et al.  Magnetic resonance imaging of perfusion using spin inversion of arterial water. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[26]  Wilson Fong.  Handbook of MRI Pulse Sequences , 2005 .