DNA barcoding and mini-barcoding in authenticating processed animal-derived food: A case study involving the Chinese market.

This study used DNA barcoding and DNA mini-barcoding to test a variety of animal-derived food products sold in the Chinese market for potential mislabeling. Samples (52) including meat, poultry, and fish purchased from retail and online sources were examined. Regions of cytochrome C oxidase I (COI) gene (~650 bp) and 16S rRNA (~220 bp) were used as full- and mini-barcode markers, respectively. Approximately 94% (49 of 52) of the samples generated barcode sequences. The failure rate for full COI full-barcodes was 44%, but we obtained the 16S rRNA mini-barcode from 87% of the COI-failed cases. Overall, the survey revealed that 23% (12 of 52) of animal-derived products were mislabeled and, in most cases, contain undeclared species. Thus, regulatory measures and continuous monitoring for mislabeling of animal-derived products should be conducted.

[1]  P. Hebert,et al.  DNA barcoding: how it complements taxonomy, molecular phylogenetics and population genetics. , 2007, Trends in genetics : TIG.

[2]  Rosalee S. Hellberg,et al.  Comparison of real-time PCR and ELISA-based methods for the detection of beef and pork in processed meat products , 2017 .

[3]  W. Kress,et al.  DNA barcoding as a reliable method for the authentication of commercial seafood products. , 2012 .

[4]  I. Khan,et al.  DNA Barcoding for the Identification of Botanicals in Herbal Medicine and Dietary Supplements: Strengths and Limitations , 2016, Planta Medica.

[5]  A. E. Wasserman,et al.  Organoleptic Identification of Roasted Beef, Veal, Lamb and Pork as Affected by Fat , 1968 .

[6]  Rosalee S. Hellberg,et al.  Identification of meat species in pet foods using a real-time polymerase chain reaction (PCR) assay , 2015 .

[7]  N. Ivanova,et al.  DNA barcoding in mammals. , 2012, Methods in molecular biology.

[8]  Sujeevan Ratnasingham,et al.  Critical factors for assembling a high volume of DNA barcodes , 2005, Philosophical Transactions of the Royal Society B: Biological Sciences.

[9]  R. S. Rasmussen,et al.  DNA-Based Methods for the Identification of Commercial Fish and Seafood Species. , 2008, Comprehensive reviews in food science and food safety.

[10]  F. Lago,et al.  Advances in authenticity testing for meat speciation , 2016 .

[11]  J. Messing.  [2] New M13 vectors for cloning , 1983 .

[12]  D. Steinke,et al.  DNA barcoding of shared fish species from the North Atlantic and Australasia: minimal divergence for most taxa, but Zeus faber and Lepidopus caudatus each probably constitute two species , 2008 .

[13]  Dipankar Ghosh,et al.  Detection of meat species adulteration using high-resolution mass spectrometry and a proteogenomics strategy , 2017, Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment.

[14]  Rosalee S. Hellberg,et al.  Identification of meat and poultry species in food products using DNA barcoding , 2017 .

[15]  M. Gadanho,et al.  Food metagenomics: Next generation sequencing identifies species mixtures and mislabeling within highly processed cod products , 2017 .

[16]  Giandomenico Corrado,et al.  Advances in DNA typing in the agro-food supply chain , 2016 .

[17]  I. Haase,et al.  Species identification in mixed tuna samples with next-generation sequencing targeting two short cytochrome b gene fragments. , 2017, Food chemistry.

[18]  D. Janzen,et al.  Pyrosequencing for Mini-Barcoding of Fresh and Old Museum Specimens , 2011, PloS one.

[19]  Rosalee S. Hellberg,et al.  DNA barcoding reveals mislabeling of game meat species on the U.S. commercial market , 2016 .

[20]  E. Garcia-Vazquez,et al.  NGS tools for traceability in candies as high processed food products: Ion Torrent PGM versus conventional PCR-cloning. , 2017, Food chemistry.

[21]  N. Z. Ballin.  Authentication of meat and meat products. , 2010, Meat science.

[22]  Mehrdad Hajibabaei,et al.  A DNA Mini-Barcoding System for Authentication of Processed Fish Products , 2015, Scientific Reports.

[23]  C. Stamatis,et al.  A new set of 16S rRNA universal primers for identification of animal species , 2014 .

[24]  Marco Arlorio,et al.  Overview on Untargeted Methods to Combat Food Frauds: A Focus on Fishery Products , 2018 .

[25]  Sajad Kiani,et al.  Fusion of artificial senses as a robust approach to food quality assessment , 2016 .

[26]  J. Spink,et al.  Introducing the Food Fraud Initial Screening model (FFIS) , 2016 .

[27]  Mehrdad Hajibabaei,et al.  Next‐generation sequencing technologies for environmental DNA research , 2012, Molecular ecology.

[28]  P. Shaw,et al.  DNA-based techniques for authentication of processed food and food supplements. , 2018, Food chemistry.

[29]  A. Belete.  Food Adulteration: Its Challenges and Impacts , 2015 .

[30]  M. Raupach,et al.  Full-length and mini-length DNA barcoding for the identification of seafood commercially traded in Germany , 2017 .

[31]  R. Marino,et al.  How meaty? Detection and quantification of adulterants, foreign proteins and food additives in meat products , 2017 .

[32]  Rosalee S. Hellberg,et al.  Identification of Species in Ground Meat Products Sold on the U.S. Commercial Market Using DNA-Based Methods , 2016 .

[33]  Mehrdad Hajibabaei,et al.  A minimalist barcode can identify a specimen whose DNA is degraded , 2006 .

[34]  Sasan Amini,et al.  Unmasking seafood mislabeling in U.S. markets: DNA barcoding as a unique technology for food authentication and quality control , 2015 .

[35]  Jeremy R. deWaard,et al.  Biological identifications through DNA barcodes , 2003, Proceedings of the Royal Society of London. Series B: Biological Sciences.

[36]  Miguel Peris,et al.  Electronic noses and tongues to assess food authenticity and adulteration , 2016 .

[37]  Mehrdad Hajibabaei,et al.  DNA barcodes for everyday life: Routine authentication of Natural Health Products , 2012 .

[38]  Shane S. Sturrock,et al.  Geneious Basic: An integrated and extendable desktop software platform for the organization and analysis of sequence data , 2012, Bioinform..