Antibacterial activity of long chain fatty acids and the reversal with calcium, magnesium, ergocalciferol and cholesterol.

SUMMARY: Screening tests indicated that Gram positive bacteria are inhibited by long chain fatty acids. No inhibition was demonstrated with Gram negative bacteria. The minimum inhibitory concentrations (MIC) for a series of the fatty acids are presented. Growth curves in the presence of linolenic acid showed increases in lag phase duration and calcium addition reversed this effect, thus indicating the arbitrary nature of the MIC values. Bactericidal studies showed lauric acid to be the most active saturated fatty acid but the activity was less than that of the C18 unsaturated fatty acids. Oleic acid was more effective than elaidic acid. Calcium ions, cholesterol and ergocalciferol reversed the activities of lauric and linoleic acids but magnesium ions effectively counteracted lauric acid only. A physicochemical explanation for the relative activities has been attempted.

[1]  H. Bean.  Types and characteristics of disinfectants. , 1967, The Journal of applied bacteriology.

[2]  E Kodicek,et al.  The effect of unsaturated fatty acids on Lactobacillus helveticus and other Gram-positive micro-organisms. , 1945, The Biochemical journal.

[3]  K. Blaxter,et al.  Modifications of the methane production of the sheep by supplementation of its diet. , 1966, Journal of the science of food and agriculture.

[4]  K. Blaxter,et al.  The effect of functional groups other than carboxyl on the metabolism of C18 and C12 alkyl compounds by sheep , 1966, British Journal of Nutrition.

[5]  M. Speck,et al.  Inhibitory Effect of Rancid Milk on Certain Bacteria , 1951 .

[6]  M. Speck,et al.  Inhibition of Streptococcus Lactis in Milk by Fatty Acids , 1951 .

[7]  J. Schulman,et al.  Molecular Interaction in Monolayers. III. Complex Formation in Lipoid Monolayers , 1938 .

[8]  W. Hamilton.  The mechanism of the bacteriostatic action of tetrachlorosalicylanilide: a Membrane-active antibacterial compound. , 1968, Journal of general microbiology.

[9]  G. Jago,et al.  The effect of fatty acids on the metabolism of lactic acid streptococci: I. Inhibition of bacterial growth and proteolysis , 1964, Journal of Dairy Research.

[10]  R. Woodroffe,et al.  The antibacterial action of tetrachlorsalicylanilide. , 1966, Journal of General Microbiology.

[11]  G. E. Morse,et al.  Long-Chain Fatty Acid Inhibition of Growth of Streptococcus agalactiae in a Chemically Defined Medium , 1966, Journal of bacteriology.

[12]  J. O. Irwin,et al.  The estimation of the bactericidal power of the blood , 1938, Epidemiology and Infection.

[13]  K. Minami.  BACTERICIDAL ACTION OF OLEIC ACID FOR TUBERCLE BACILLI , 1957, Journal of bacteriology.

[14]  J. Czerkawski,et al.  Fermentation of various soluble carbohydrates by rumen micro-organisms with particular reference to methane production , 1969, British Journal of Nutrition.

[15]  C NIEMAN,et al.  Influence of trace amounts of fatty acids on the growth of microorganisms. , 1954, Bacteriological reviews.

[16]  M. Dunn,et al.  Saturated fatty acids as bacterial antimetabolites. , 1957, Archives of biochemistry and biophysics.

[17]  M. Frobisher.  Relations of Surface Tension to Bacterial Phenomena , 1926 .

[18]  H. Lichstein,et al.  THE INHIBITION OF THE MALIC ENZYME OF LACTOBACILLUS ARABINOSUS 17-5 BY OLEIC ACID. II. PREVENTION OF ENZYME INHIBITION. , 1963, Archives of biochemistry and biophysics.

[19]  W. Hugo.  The mode of action of antibacterial agents. , 1967, The Journal of applied bacteriology.

[20]  J. Foster,et al.  Studies on the effects of C18 unsaturated fatty acids on growth and respiration of Micrococcus pyogenes var aureus. , 1950, The Journal of infectious diseases.

[21]  R. Hotchkiss.  THE NATURE OF THE BACTERICIDAL ACTION OF SURFACE ACTIVE AGENTS , 1946 .