Optical Analysis of Implants from the Dura Mater

Presents the results of the spectral analysis using the method of Raman scattering spectroscopy (RS) of dura mater (DM) samples, manufactured by technology “Lioplast” practised in the clinic in the area of atrophic processes at multiple gum recessions. The method of Fourier deconvolution and selection of the spectral profile by the method of least squares is used to increase the resolution and informativity of the spectrum. With the help of mathematical methods of separation of overlapping spectral contours, the main bands corresponding to the main components of the implants were found: amides, proteins, glycosaminoglycans, DNA/RNA. On the basis of the two-dimensional spectral analysis, the coefficients reflecting the composition of the dura mater with different methods of its treatment were introduced. It has been established that Raman spectroscopy can be used to evaluate implants from the dura mater.

[1]  Pavel E. Timchenko,et al.  Spectral analysis of allogeneic hydroxyapatite powders , 2017 .

[2]  George J. Thomas,et al.  Raman, polarized Raman and ultraviolet resonance Raman spectroscopy of nucleic acids and their complexes , 2005 .

[3]  D. McLean,et al.  Automated Autofluorescence Background Subtraction Algorithm for Biomedical Raman Spectroscopy , 2007, Applied spectroscopy.

[4]  Zhiwei Huang,et al.  Early detection of biomolecular changes in disrupted porcine cartilage using polarized Raman spectroscopy. , 2011, Journal of biomedical optics.

[5]  A. Christopoulos,et al.  Fitting Models to Biological Data Using Linear and Nonlinear Regression: A Practical Guide to Curve Fitting , 2004 .

[6]  Miguel Ángel Medina,et al.  Characterization by Raman spectroscopy of conformational changes on guanine–cytosine and adenine–thymine oligonucleotides induced by aminooxy analogues of spermidine , 2004 .

[7]  David I. Ellis,et al.  Illuminating disease and enlightening biomedicine: Raman spectroscopy as a diagnostic tool. , 2013, The Analyst.

[8]  Pavel E. Timchenko,et al.  Detailed spectral analysis of decellularized skin implants , 2016 .

[9]  E. V. Timchenko,et al.  Optical analysis of aortic implants , 2016, Optical Memory and Neural Networks.

[10]  FT-Raman signatures of genomic DNA from plant tissues , 2009 .

[11]  K. Nakamoto,et al.  Introductory Raman Spectroscopy , 1994 .

[12]  S. Lane,et al.  Micro-Raman spectroscopy detects individual neoplastic and normal hematopoietic cells. , 2006, Biophysical journal.

[13]  Joseph Chaiken,et al.  Raman spectroscopic investigation of spinal cord injury in a rat model. , 2011, Journal of biomedical optics.

[14]  Hao Chen,et al.  In-vivo spinal nerve sensing in MISS using Raman spectroscopy , 2016, SPIE Smart Structures and Materials + Nondestructive Evaluation and Health Monitoring.

[15]  E. Yates,et al.  Raman and Raman optical activity of glycosaminoglycans. , 2010, Chemical communications.

[16]  Li Duan,et al.  Extracellular matrix production in vitro in cartilage tissue engineering , 2014, Journal of Translational Medicine.