A. E. Jofre-Garfias N. Villegas-Sepúlveda J. L. Cabrera-Ponce R. M. Adame-Alvarez L. Herrera-Estrella J. Simpson Agrobacteriummediated transformation of Amaranthus hypochondriacus: light- and tissue-specif ic expression of a pea chlorophyll alb-binding protein promoter

Mature embryos of Amaranthus hypochondri- acus (amaranth) were used to develop an in vitro culture system for plant regeneration and genetic transformation. Plants were regenerated from embryo-derived callus cul- tivated on Murashige and Skoog medium supplemented with 10 pkf 2,4-dichlorophenoxyacetic acid or 3,6-dich- loro-2-methoxybenzoic acid and 10% coconut liquid en- dosperm. Transgenic plants were obtained by inoculation of mature embryo explants with a disarmed Agrobacterium strain containing the plasmid pGV2260(pEsc4), which carried the genes encoding neomycin phosphotransferase type 11 and P-glucuronidase. The presence of transgenes in the genome of transformed amaranth plants and their prog- eny was demonstrated by Southern blot hybridization. Tis- sue specific and light-inducible expression directed by a pea chlorophyll a/b-binding protein promoter was ob- served in transgenic amaranth plants and their progeny.

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