A 64 pixel ISFET-based biosensor for extracellular pH gradient monitoring

We present an integrated biosensor platform using ion-sensitive field effect transistor (ISFET) device for monitoring pH levels in cell culture medium. Each pixel in the 8×8 array employs a pair of p-type and n-type ISFETs sharing the same ion sensitive membrane. The complementary pair of ISFETs works as a chemical switch that can be used for pH thresholding. The proposed biosensor is fabricated using TSMC 0.35 μm standard CMOS technology. We show the functionality and sensitivity of device by using buffer solutions with different pH values. The proposed biosensor is functional in the wide pH range of 1 to 14 with the sensitivity of 50 mV/pH. Due to its low complexity, pH sensitivity, and biocompatibility, this platform can be used for cellular studies applications such as cancer cell growth monitoring.

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